Abstract: We evaluated the effects of plant types and soil physicochemical properties on the distribution of Acidobacteria in the rhizosphere soil. Soil samples were collected from the mixed evergreen and deciduous forest of Mey Blossom Garden, Wuxi City. The Illumina MiSeq high-throughput sequencing technology was adopted to analyze the relative abundance and subgroup distribution of Acidobacteria. The results showed that Acidobacteria was the second most abundant bacterial group, accounting for 15.4%-28.78% of the total. We obtained 20 acidobacterial subgroups from seven soil samples. The Gp1, Gp2, Gp3, Gp4, Gp5, Gp6 were the dominant acidobacterial subgroups, accounting for 80.23%-94.85% of the acidobacterial sequences. Gp1, Gp2 and Gp3 were the predominant subgroups in the evergreen tree rhizosphere soils, while Gp4, Gp5 and Gp6 were dominant in the deciduous tree soils. The subgroup Gp14 was only found in the rhizosphere soils of evergreen trees, while Gp9, Gp18, Gp20 and Gp22 were merely found in the deciduous tree soils. The results indicated that the plant types could affect the distribution of acidobacterial subgroups in rhizosphere soils. Among the soil physicochemical indices, the abundance of Acidobacteria was significantly influenced by total nitrogen content (P<0.05), and the distribution of soil acidobacterial subgroups was significantly affected by soil pH (P<0.01). The results suggested that the acidobacterial community composition and diversity were predominantly affected by soil pH, total nitrogen content, and tree types. Soil pH was the key factor influencing acidobacterial diversity.

Key words: optimized fertilization, influencing factor, environmental benefit, nitrogen and phosphorus losses, rice