Abstract: The yellow-spined bamboo locust, Ceracris kiangsu (Orthoptera: Oedipodidae) is an important bamboo pest in south China. To better understand the mechanism of the urine puddling behavior of C. kiangsu, we examined the EAG responses of C. kiangsu adults towards the volatiles of human urine fermented for different days and the solutions of ammonium bicarbonate and indole. The results showed that the EAG responses of C. kiangsu adults towards human urine volatiles were influenced significantly by the duration of human urine fermentation. Compared with fresh urine and human urine fermented for 7, 15, 30, 45 and 60 days, the volatiles emitted from human urine incubated for 30 days elicited the strongest EAG response in both female and male adults. In addition, the solutions of indole, ammonium bicarbonate and urine in different concentrations could evoke strong EAG response in male and female adults respectively. The EAG response elicited by different chemical solutions were significantly weaker than that evoked by human urine (P<0.05). The EAG responses towards the volatiles emitted from human urine and chemical solutions were affected by sex, and the human urine fermented for 0 and 15 days elicited stronger EAG responses in females than in males, while the EAG responses evoked by the human urine and the solutions of ammonium bicarbonate and indole were respectively weaker in females than in males.

Key words: Pinus massoniana, fine root, production, turnover.