Cloning and bioinformatic analysis of signal-degrading enzyme gene aiiA from marine bacterium ZD02.

Abstract

Abstract: By using a pair of degenerate primers designed according to the known conserved sequence of aiiA, the gene aiiA encoding the quorum sensing signal degrading enzyme (AiiA) in the genome of marine bacterium ZD02 was amplified by PCR, and the PCR products (ZD02 aiiA) were cloned, sequenced, and analyzed. The ZD02 aiiA was 753 bp (Genbank accession number: KC756214), containing an open reading frame (ORF) which encoded a polypeptide chain AiiA of 250 amino acids residues with a molecular weight of 28.1 kDa and an isoelectric point value of 4.78. A conservative domain (Lactamase-B) (34AA-235AA) in the sequence was identified, and the 3D structure of deduced AiiA protein was further predicted. This study would provide a foundation for the expression of the sequence and its bio-activity analysis.

Key words: , rice, melatonin, Cd, chemical form.

DING Xian1**, SUN Wei-wen2, ZHANG Dian-chang1, WENG Xiong1, LIN Hei-zhe1, ZHOU Shi-ning2. Cloning and bioinformatic analysis of signal-degrading enzyme gene aiiA from marine bacterium ZD02.[J]. cje.

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Cloning and bioinformatic analysis of signal-degrading enzyme gene aiiA from marine bacterium ZD02.

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