Abstract: Overwintering and early rearing of Latrodectus tredecimguttatus baby spiders is the key of artificially rearing the spiders. Through the comparison of the hatch rate, survival rate, and average body mass of overwintered baby spiders under different preservation temperature and duration, this paper studied the overwintering pattern of L. tredecimguttatus baby spiders, and the effects of relative humidity, environmental temperature and food on the early growth and development of the baby spiders after leaving egg bags. The results showed that the optimal temperature for the preservation and overwintering of the egg bags was around 4 ℃, but the survival rate of baby spiders decreased gradually after 7 months of the preservation under this temperature, and got to zero after 13 months. To let the eggs hatch out, egg bags should be placed indoor for a period of time (around 2 months) before preserved at low temperature. After left egg bag and reared with Tenerio molitor for four weeks, the average body mass of overwintering baby spiders under 55%-60% relative humidity and 25 ℃-30 ℃ increased by 40- or more fold, with a survival rate greater than 80%.

Key words: Cymbopogon citratus, Volatile, GC-MS, Allelopathy