Optmization for the reaction system of birch ISSR-RCR

Abstract

Abstract: In this paper,the influence of DNA concentration, Tag DNA polymerase and annealing temperature on the result of ISSR-PCR amplification was analyzed using the materials of birch DNA. Seventeen primers, which produced clear and rich polymorphism bands, were screened. The stable and reproducible optimum reaction system of ISSR PCR and PCR amplification parameter were established for birch. The result provided a standardizing program for the analysis of interspecies genetic diversity of Birch.

Key words: Wheat, Drought, Waterlogging, Nitrogen supply, Photosynthesis, Starch accumulation

CLC Number:

S792.153

JIANG Jing, YANG Chuanping, LIU Guifeng, WU Jinhua, FAN Zhiyong. Optmization for the reaction system of birch ISSR-RCR[J]. cje, 2003, (3): 91-93.

[1]	KOU Na, ZHAO Qiong, PENG Binwei, TANG Qiang, SONG Jing, JIANG Hongqiang, WU Wenge. Impacts of long-term straw return on soil carbon stability and fertility in rice-wheat rotation system. [J]. Chinese Journal of Ecology, 2026, 45(6): 1810-1817.
[2]	HE Peijin, SUN Anni, LI Lin, XU Qingzhe, HUI Mengmeng, GAO Xining, WANG Liwei, YIN Hong. Effects of drought stress and rehydration on leaf photosynthetic characteristics, quality and yield of soybean. [J]. Chinese Journal of Ecology, 2026, 45(6): 1870-1879.
[3]	CHEN Changfan, JIANG Jie, QIU Chenggang, TIAN Jiao, YANG Hanjun, WANG Xuemei, WEI Qing, CHEN Jinsong. Transgenerational effects of drought on morphological and photosynthetic traits between an invasive clonal plant and its congeneric native species. [J]. Chinese Journal of Ecology, 2026, 45(5): 1444-1453.
[4]	XU Ge, LI Jiawen, ZENG Wenxi, YAN Meijing, DONG Tingfa. Effects of lethal drought on the metabolic dynamics of non-structural carbohydrates in Rhododendron decorum. [J]. Chinese Journal of Ecology, 2026, 45(5): 1454-1462.
[5]	HUANG Yuting, YANG Yuantong, ZHU Jieyi, SU Licheng, LUO Zhizhong, ZENG Shucai. Physiological responses and drought resistance mechanisms of Cinnamomum camphora seedlings under different soil moisture. [J]. Chinese Journal of Ecology, 2026, 45(5): 1508-1516.
[6]	YANG Xiaofei, ZHANG Ye, ZHANG Siyang, ZHANG Wenting. Effects of salt and alkali stress on seed germination and physiological characteristics of wheat germplasm materials. [J]. Chinese Journal of Ecology, 2026, 45(5): 1533-1543.
[7]	LI Jianming, ZHOU Xuhui, LI Sheng, ZHOU Yufei, ZHOU Benzhi, CAO Yonghui. Seasonal variations of leaf water potential in Phyllostachys edulis of different ages under long-term drought stress. [J]. Chinese Journal of Ecology, 2026, 45(4): 1245-1253.
[8]	QIN Cheng, XI Wenjie, NIU Peiyao, ZHANG Xiaxin, SHEN Jie, LI Qiang, LIAN Huida. The synergistic effect of exogenous selenium and nitric oxide in alleviating drought stress in tomato seedlings: Physiological responses and gene expression mechanisms. [J]. Chinese Journal of Ecology, 2026, 45(4): 1280-1289.
[9]	HUANG Jin, ZHANG Fangmin. Analysis and prediction of precipitationinduced disasters during the critical stage of winter wheat in Henan Province. [J]. Chinese Journal of Ecology, 2026, 45(2): 644-652.
[10]	ZHANG Jing, ZHANG Nan, ZHANG Huiyu, XIE Lili, ZHANG Dingyi. Response of root tiller development and yield formation of extremely-late sowing wheat to the interactions of planting density and nitrogen application. [J]. Chinese Journal of Ecology, 2026, 45(1): 192-201.
[11]	WANG Zixu, LUO Chunyan, GUO Ziyan, TONG Yuhang, ZHENG Weijun, LI Qingwei. Photosynthetic responses of seedlings from two Prunus mume cultivars to drought and subsequent rewatering. [J]. Chinese Journal of Ecology, 2025, 44(8): 2608-2617.
[12]	ZHOU Meng, LI Baozhan, CHEN Hui, CHAI Yuwei, YANG Yong, ZHANG Senyu, WANG Zeyi, CHANG Lei, HUANG Caixia. Effects of straw strip mulching coverage on winter wheat yield and soil water and heat distribution in Loess Plateau. [J]. Chinese Journal of Ecology, 2025, 44(8): 2618-2628.
[13]	CHEN Siyi, LI Junjian, YIN Yaxin, SHAN Qiaobo, ZHENG Chunfang. Physiological mechanism of low temperature stress inhibiting photosynthesis in Aegiceras corniculatum seedlings. [J]. Chinese Journal of Ecology, 2025, 44(6): 1866-1873.
[14]	SU Ya, WANG Haijuan, WANG Hongbin. Accumulation, tolerance, and photosynthetic response of cadmium in Eclipta prostrata. [J]. Chinese Journal of Ecology, 2025, 44(5): 1581-1591.
[15]	SHEN Jie, SONG Xiaojing, ZHONG Dandan, QIN Cheng, PEI Penggang, WANG Xi, LEI Haiying, HAO Xingyu. Effects of humic acid and D-arginine on root growth of foxtail millet (Setaria italica) seedlings under drought stress. [J]. Chinese Journal of Ecology, 2025, 44(4): 1220-1225.

Optmization for the reaction system of birch ISSR-RCR

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments