Abstract: To clarify the safety and effectiveness of otolithic strontium markers in juvenile Liza haematocheila, otolith marking was carried out- through soaking in different concentrations (20-100 mg·L^-1) of strontium chloride hexahydrate (SrCl₂·6H₂O) for 1, 2, and 3 d. After that the marking juvenile fish were transferred to natural seawater for 90 d of continuing rearing. The results showed no significant difference in Sr/Ca ratio between the all marking groups and the control groups. After these fish were reared for 90 d, there was no significant difference in Sr/Ca ratio between the marked ST1-20 marking groups and control groups, while the Sr/Ca ratio of the other marking groups in area 1.1-1.3 mm away from the otolith core was significantly higher than that of the control groups. After being reared for 90 d, the effect of the Sr-marking was still visible. During the experiment period, there were no significant differences in body length, body weight, fullness and death rate between the marked groups and the control groups. For the groups, after 40 mg·L^-1 Sr-marking for 1 d and 20 mg·L^-1 Sr-marking for 2 d, the muscle strontium content in the two marked groups gradually returned to the control level at 36 and 38 d. Our results suggested that the otolith Sr-marking method for juvenile Liza haematocheila could be effective, stable, and safe. The better marker groups are ST1-40 and ST2-20.