摘要:
采用富集培养分离法,从烟样湖南A3B1(2013)、云南C3F(2013)中分离出3株氨化细菌,编号GZUIFR-YC01、GZUIFR-YC02、GZUIFR-YC03。通过形态特征、生理生化特性和基于16S rDNA的系统学分析,鉴定菌株。应用纳氏试剂显色法初步研究3株菌的氨化能力。结果表明:菌株GZUIFR-YC01为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia),GZUIFR-YC02为纺锤形赖氨酸芽孢杆菌(Lysinibacillus fusiformis),GZUIFR-YC03为副短短芽孢杆菌(Brevibacillus parabrevis)。菌株GZUIFR-YC02长势优于菌株GZUIFR-YC01和GZUIFR-YC03,同时除氮能力明显高于其他两菌株。研究结果可为烟叶醇化生态过程中微生物驱动的氮元素转化提供参考。
周家喜1,张晓敏2,胡大鸣2,惠建权2,余涛1,邹晓1*. 陈化烟叶中氨化细菌鉴定及有机氮降解特性[J]. 生态学杂志.
ZHOU Jia-xi1, ZHANG Xiao-min2, HU Da-ming2, HUI Jian-quan2, YU Tao1, ZOU Xiao1*. Isolation and identification of ammonifying bacteria in the flue-cured tobacco and characteristics of degrading organic nitrogen.[J]. cje.