四个大豆栽培种的花序转录组分析

生态学杂志

四个大豆栽培种的花序转录组分析

张驰^1,2,高振蕊^1,2,董友魁³,崔敏龙^1**

(¹中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室，沈阳 110016； ^2中国科学院大学，北京 100049； ^3辽宁省铁岭市农业科学院，辽宁铁岭 112616)

出版日期:2015-12-10 发布日期:2015-12-10

Transcriptome analysis of inflorescences from four soybean cultivars.

ZHANG Chi^1,2, GAO Zhen-rui^1,2, DONG You-kui³, CUI Min-long^1**

(¹Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ²University of Chinese Academy of Sciences, Beijing 100049, China; ^3Tieling Academy of Agricultural Sciences in Liaoning Province, Tieling 112616, Liaoning, China)

Online:2015-12-10 Published:2015-12-10

摘要/Abstract

摘要： 针对大豆落花落荚问题，选取花器官脱落性质存在差异的4份大豆(Glycine max)花序样品进行了RNA提取、转录组测序和分析。共获得原始数据20 GB，其中有效数据14.87 GB。结果表明，多荚与少荚品种花序转录水平差异主要集中在物质代谢、胁迫应答（GLYMA02g03301、GLYMA02G03230等）、刺激应答、病害抗性等方面，与脱落直接相关的基因表达上并无显著差异，表明参与上述生理过程的相关基因表达的差异与不同品种大豆落花落荚性质上的差异具有一定相关性。该结果系统地了解了不同品种大豆在花期花序部分的转录组特征与差异，分析了相关表达差异对于大豆落花落荚的影响，同时丰富了大豆转录组数据信息。

关键词: 阿维菌素, 亚致死效应, 甲氰菊酯, 生命表参数, 柑橘全爪螨

Abstract: To study the floral organ abscission, soybean (Glycine max) inflorescences from four cultivars were used for RNA extraction, RNAseq and transcriptome analysis. A total of 20 GB raw data and 14.87 GB clean data were obtained from RNAseq. The result showed that the transcriptome differences between multiple and relatively less soybean inflorescences mainly lied in substance metabolism, stress response (GLYMA02g03301, GLYMA02G03230, etc.), stimulation response and disease resistance. But genes directly related to abscission show no expression difference. This indicated the possible influences of these genes on soybean flowers and pods abscission. Our research systematically investigated the characteristics and variances of different soybean inflorescences and analyzed the effects of different expression genes on soybean flower abscission and shattering. These data enriched soybean transcriptome database.

Key words: fenpropathrin, Panonychus citri, avermectin, life-table parameters., sublethal effect

张驰1,2,高振蕊1,2,董友魁3,崔敏龙1**. 四个大豆栽培种的花序转录组分析[J]. 生态学杂志.

ZHANG Chi1,2, GAO Zhen-rui1,2, DONG You-kui3, CUI Min-long1**. Transcriptome analysis of inflorescences from four soybean cultivars.[J]. cje.

[1]	陈家长,杨光,孟顺龙,马晓燕,胡庚东. 不同浓度阿维菌素对鲤鱼过氧化氢酶和谷胱甘肽-S-转移酶活性的影响 [J]. 生态学杂志, 2010, 29(04): 680-686.
[2]	刘学涛;刘峰;慕卫;魏光;李燕平 . 紫外吸收剂抑制阿维菌素光解及对小菜蛾防治的增效作用 [J]. 生态学杂志, 2006, 25(02): 185-188 .