Abstract: Evaluation of regional species diversity is one of the important components of the global biodiversity research. Studies on vertical distribution pattern of amphibian species richness could provide a scientific basis for biodiversity conservation. In order to understand the amphibian biodiversity and the altitudinal distribution characteristics in Wuyishan Nature Reserve, we surveyed the amphibians in 22 sample lines from 300 to 2161 m, which were divided into 10 sections for every 200 m from July 22 to August 4, 2014. The results showed that: (1) Thirtytwo species of amphibians belonging to 22 genera, 9 families, 2 orders were recorded, of which the Ranidae (37.9% of total species) was the predominant family. (2) Amolops chunganensis was a new record in the nature reserve. (3) With the increase of altitude, the amphibian richness presented a plateau pattern then decreased, which was mainly influenced by habitat complex. Meanwhile, the Stevens and crossspecies methods were used to examine whether Rapoport’s rule is applicable to speciesspecific elevational range sizes of amphibians. The results suggested that amphibian range in this nature reserve did not support Rapoport’s rule.