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不同生态型摩西球囊霉菌株对蜈蚣草砷吸收的影响

吴福勇1,2**,刘雪平1,毕银丽3,郭一飞1,毛艳丽1,黄铭洪2   

  1. (1河南城建学院环境与市政工程系, 河南平顶山 467036; 2香港浸会大学裘槎环科所, 香港; 3中国矿业大学(北京)地球科学与测绘工程学院, 北京 100083)
  • 出版日期:2013-06-10 发布日期:2013-06-10

Effects of different ecotype Glomus mosseae isolates on arsenic uptake by Pteris vittata.

WU Fu-yong1,2**, LIU Xue-ping1, BI Yin-li3, GUO Yi-fei1, MAO Yan-li1, WONG Ming-hong2   

  1. (1Department of Environmental and Municipal Engineering, Henan University of Urban Construction, Pingdingshan 467036, Henan, China; 2Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Hong Kong, China; 3College of Geoscience and Surveying Engineering, China University of Mining and Technology, Beijing 100083, China)
  • Online:2013-06-10 Published:2013-06-10

摘要: 砷超富集植物——蜈蚣草无论是在野外或是在室内均能被丛枝菌根真菌(AM真菌)侵染,但其对蜈蚣草砷吸收及转运的机理尚不清晰。本研究将分离于湖南省郴州市金川塘某铅锌尾矿蜈蚣草根际土壤(Glomus mosseae BGC GD01,简称污染菌株)和云南省未污染土壤(G. mosseae BGC YN05,简称非污染菌株)的2种摩西球囊霉菌株分别接种于非污染生态型和污染生态型蜈蚣草根际,8周后利用菌根化蜈蚣草幼苗在浓度为100 μmol·L-1砷(Na2HAsO4·7H2O)营养液中进行为期24 h的水培试验。结果表明,2种生态型摩西球囊霉菌株分别与蜈蚣草形成中等程度侵染,侵染率为25.2%~31.3%。无论是接种污染菌株或是非污染菌株,均明显促进了蜈蚣草根部对磷的吸收。在24 h水培试验期间,接种非污染菌株显著促进了蜈蚣草根部砷的吸收,但接种污染菌株对蜈蚣草根部砷吸收的促进作用有限,说明AM真菌对蜈蚣草砷吸收存在种内差异。

关键词: 微生物降解, 藻毒素, 耦合工艺, 天然有机物

Abstract: Pteris vittata, as an arsenic (As) hyperaccumulator, can be colonized by arbuscular mycorrhizal (AM) fungi either in controlled conditions or at field sites. However, the physiological mechanisms of AM fungi affecting the As accumulation and translocation in P. vittata are not fully elucidated. In this study, two isolates of Glomus mosseae were obtained from the rhizosphere of P. vittata growing on an Ascontaminated site in Jinchuantang of Hunan Province (G. mosseae BGC GD01A, metalcontaminated isolate) and from the clean soil in Yunnan Province (G. mosseae BGC YN05, uncontaminated isolate). The isolates were inoculated to the rhizosphere of Ascontaminated and uncontaminated P. vittata, respectively. After 8 weeks of inoculation, a 24h water culture experiment was conducted with the P. vittata seedlings colonized by AM fungi and exposed to 100 μmol·L-1 of As (Na2HAsO4·7H2O). Both the two isolates could colonize on P. vittata roots at moderate level (25.2%-31.3%). The inoculation either with metalcontaminated isolate or with uncontaminated isolate promoted the P uptake by P. vittata roots obviously. During the 24 h culture, the inoculation with uncontaminated isolate promoted the As uptake by P. vittata roots significantly, while the inoculation with metalcontaminated isolate only had a slight effect in promoting the As uptake, showing that there was an intraspecific difference of AM fungi in their effects on the As uptake by P. vittata.

Key words: cyanobacterial toxin, coupling process, microbial degradation, natural organic matter.