大豆根腐病生防菌KJB04-11的鉴定及其产生的脂肽类抗生素

生态学杂志 ›› 2012, Vol. 31 ›› Issue (06): 1453-1460.

大豆根腐病生防菌KJB04-11的鉴定及其产生的脂肽类抗生素

李旭,马福海,王秀娟,孙健,张惠文^**

（中国科学院沈阳应用生态研究所，沈阳 110016）

出版日期:2012-06-09 发布日期:2012-06-09

Identification of bio-control bacterial strain KJB04-11 against soybean root rot disease and the produced lipopeptide antibiotics by the strain.

LI Xu, MA Fu-hai, WANG Xiu-juan, SUN Jian, ZHANG Hui-wen^**

(Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China)

Online:2012-06-09 Published:2012-06-09

摘要/Abstract

摘要： 从大豆根围筛选到1株对尖孢镰刀菌和立枯丝核菌都具有很好拮抗作用的菌株KJB0411，经形态观察、生理生化特征和16S rDNA序列分析，属于枯草芽孢杆菌（Bacillus subtilis）。具有抗菌活性的KJB0411发酵液无菌滤液对热和酸碱具有较强的稳定性。采用Sephadex G25柱层析、反相HPLC和冷冻干燥从KJB04-11发酵液中分离纯化了抗菌活性成分。由红外光谱、MALDI-TOF-MS、氨基酸组成及脂肽合成酶基因扩增结果推测该菌株产生的抗菌物质为C₁₆、C₁₇的mycosubtilin和C₁₅的surfactin。田间试验表明，大豆种子经KJB04-11发酵液包衣处理对大豆根腐病防效为53.6%，大豆产量提高12.5%。

关键词: 河口近海, 桡足类, 休眠卵, 滞育卵

Abstract: A bacterial strain KJB04-11 with antagonistic effect against Fusarium oxysporum and Rhizoctonia solani was isolated from soybean rhizosphere. The strain was identified as Bacillus subtilis, based on its morphological, cultural, physiological, and biochemical characteristics, and 16S rDNA sequence analysis. The fermented filtrate with antagonistic activity showed certain thermostability and certain acidic and alkaline stability in the range of pH 1.0-13.0. The antagonistic active compositions in the fermented filtrate were purified by gel filtration on Sephadex G₂₅ column and RF-HPLC, and preliminarily identified as C16 mycosubtilin, C₁₇ mycosubtilin, and C₁₅ surfactin by infrared spectra, MALDI-TOF mass spectrum, amino acid composition analysis, and amplification of lipopeptide synthase gene. In field trial, the control efficacy of seed coating agent prepared with KJB04-11 against soybean root rot disease was 53.6%, and the soybean yield was increased by 12.5%

Key words: estuarine and coastal area, copepod, resting egg, diapause egg.

李旭,马福海,王秀娟,孙健,张惠文**. 大豆根腐病生防菌KJB04-11的鉴定及其产生的脂肽类抗生素[J]. 生态学杂志, 2012, 31(06): 1453-1460.

LI Xu, MA Fu-hai, WANG Xiu-juan, SUN Jian, ZHANG Hui-wen**. Identification of bio-control bacterial strain KJB04-11 against soybean root rot disease and the produced lipopeptide antibiotics by the strain.[J]. cje, 2012, 31(06): 1453-1460.

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