PAHs污染土壤的物理强化修复过程中土壤细菌的响应

生态学杂志

PAHs污染土壤的物理强化修复过程中土壤细菌的响应

宋宇¹,王健^2,3,苏振成^2*,张惠文^2

(¹辽东学院，辽宁丹东 118003； ²中国科学院沈阳应用生态研究所，沈阳 110164； ³中国科学院大学，北京 100049)

出版日期:2016-06-10 发布日期:2016-06-10

Response of bacterial community in PAHs contaminated soil during physically enhanced remediation.

SONG Yu¹, WANG Jian^2,3, SU Zhen-cheng^2*, ZHANG Hui-wen^2

(¹Eastern Liaoning University, Dandong 118003, Liaoning, China; ² Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China; ^3University of Chinese Academy of Sciences, Beijing 100049, China）.

Online:2016-06-10 Published:2016-06-10

摘要/Abstract

摘要： 采用变性梯度凝胶电泳和荧光定量real-time PCR方法，研究了切换电场对多环芳烃(PAHs)污染土壤进行物理强化修复时土壤中细菌群落的响应。结果表明：施加切换电场有利于土壤PAHs的降解，电极周围的土壤中PAHs的降解率均在50%以上，且电场中心的效果更好。修复过程中土壤中细菌16S rRNA基因拷贝数和多样性指数在电极附近和电极中心土壤之间差异不明显，与对照亦没有显著差异；但细菌种群结构在电极附近和电极中心土壤之间却有明显差异，与对照之间差异尤为显著。施加电场后土壤细菌种群结构的改变可能是这一过程的微生物学机制之一。

关键词: 染色试验, 染色路径数目, 裂隙面积密度, 地表特征

Abstract: Responses of bacterial community in polycyclic aromatic hydrocarbon (PAHs) contaminated soil remedied by reversal electric field were assessed by denaturing gradient gel electrophoresis (DGGE) and realtime quantitative PCR. The results showed that reversal electric field was favorable to the degradation of PAHs. The degradation rates of PAHs in the soils near the electrode and at electric field center were both more than 50%, especially in the soils at electric field center. During the remediation, bacteria 16S rRNA gene copy numbers and diversity indices of bacterial communities had no significant change between the soils near the electrode and at electric field center, and also showed no significant difference compared with the control. However, bacterial community structure showed obvious difference between the soils near the electrode and at electric field center, and had greater change compared with the control. The results suggested that changes in composition of bacterial community after application of electric field might be one of microbiological mechanisms of electro-enhanced bioremediation.

Key words: dye tracer experiment, stained path number, crack area density, soil surface characteristics.

宋宇1,王健2,3,苏振成2*,张惠文2. PAHs污染土壤的物理强化修复过程中土壤细菌的响应[J]. 生态学杂志.

SONG Yu1, WANG Jian2,3, SU Zhen-cheng2*, ZHANG Hui-wen2. Response of bacterial community in PAHs contaminated soil during physically enhanced remediation.[J]. cje.