商陆根超声波萃取物对朱砂叶螨的生物活性

生态学杂志

商陆根超声波萃取物对朱砂叶螨的生物活性

丁丽娟,丁伟^**,张永强

(西南大学植物保护学院，重庆 400716)

出版日期:2013-03-10 发布日期:2013-03-10

Acaricidal activity of different ultrasonic wave extracts from Phytolacca americana roots against Tetranychus cinnabarinus.

DING Li-juan, DING Wei^**, ZHANG Yong-qiang

(College of Plant Protection, Southwest University, Chongqing 400716, China)

Online:2013-03-10 Published:2013-03-10

摘要/Abstract

摘要： 比较了商陆(Phytolacca americana)根丙酮提取物的正丁醇和乙酸乙酯2种溶剂超声波萃取液及其残留物对朱砂叶螨(Tetranychus cinnabarinus)的生物活性。结果表明，商陆根正丁醇萃取液的活性最好，以浓度为2.5 mg·mL^-1处理朱砂叶螨雌成螨24和48 h的校正死亡率分别为74%和97%；处理48 h的LC₅₀为1.787 mg·mL^-1，LC₉₀为7.272 mg·mL^-1；浓度为1、2和4 mg·mL^-1的正丁醇萃取液对雌成螨24 h的驱避率分别为62%、82%和84%；同时对雌成螨48 h的产卵抑制率分别为39%、81%和93%。进一步对商陆根正丁醇萃取液柱层析分离和TLC鉴定获得14个组分，其中以组分11的杀螨效果最好，浓度为2 mg·mL^-1时，处理48 h，对朱砂叶螨雌成螨的校正死亡率达到了98%，LC₅₀为0.932 mg·mL^-1，LC₉₀为3.110 mg·mL^-1。

关键词: 宏蛋白质组学, 代谢途径, 降解机制, 微生态, 废水生物处理

Abstract: Two ultrasonic wave extracts (n-butarol and ethyl acetate as solvents) of Phytolacca americana acetone extract and residues after extracting were investigated for acaricidal activities against Tetranychus cinnabarinus. Results showed that the n-butarol extract exhibited the highest acaricidal activity among all treatments. The corrected mortalities of n-butarol extract were 74% and 97% after 24 h and 48 h of treatment at 2.5 mg·mL^-1, and its LC₅₀ and LC₉₀ were 1.787 and 7.272 mg·mL^-1 for 48 h, respectively. Meanwhile, the repellent rates were 62%, 82% and 84% against the mite for 24 h and oviposition inhibition rates (48 h) were 39%, 81% and 93%, at concentrations of 4, 2 and 1 mg·mL^-1, respectively. The nbutarol extract was further separated by the silica gel column and monitored by thinlayer chromatography (TLC) with UV detection, and 14 fractions were obtained. The 11th fraction showed highest acaricidal activity with a corrected mortality of 98% at the concentration of 2 mg·mL^-1 and its LC₅₀ and LC₉₀ were 0.932 and 3.110 mg·mL^-1, respectively after 48 h of treatment.

Key words: metaproteomics, metabolic pathways, biodegradation mechanism, microbial ecosystems, biological treatment of wastewater.

丁丽娟,丁伟**,张永强. 商陆根超声波萃取物对朱砂叶螨的生物活性[J]. 生态学杂志.

DING Li-juan, DING Wei**, ZHANG Yong-qiang. Acaricidal activity of different ultrasonic wave extracts from Phytolacca americana roots against Tetranychus cinnabarinus.[J]. cje.

[1]	郭栗村, 烟征, 可欣. 钛酸盐负载Ag型光催化剂降解罗丹明B [J]. 生态学杂志, 2023, 42(7): 1749-1754.
[2]	郑学昊,孙丽娜,刘克斌,孙家君,王晓旭,郑佳玉,马欣雨,张鸿龄,王辉. 根际促生菌及木质素对持久性有机污染土壤修复的调控和酶活性的影响 [J]. 生态学杂志, 2018, 37(6): 1807-1814.
[3]	路秀蓉1,刘婷1,王瑞1,王惠1*,段玉玺2. 外来入侵植物三裂叶豚草凋落叶分解对土壤线虫群落结构的影响 [J]. 生态学杂志, 2016, 35(9): 2369-2378.
[4]	范立民1,2,徐跑1,2,吴伟1,2,瞿建宏1,裘丽萍1,陈家长1,2**. 淡水养殖池塘微生态环境调控研究综述 [J]. 生态学杂志, 2013, 32(11): 3094-3100.
[5]	孙秋玲1,2,戴思兰1,张春英2**,魏翔莺2,3. 菌根真菌促进植物吸收利用氮素机制的研究进展 [J]. 生态学杂志, 2012, 31(5): 1302-1310.
[6]	陈登科,李丽立,张彬,张复生,李洪庆,蒋金津. 不同饲料添加剂对樱桃谷鸭生长性能及鸭舍有害气体含量的影响 [J]. 生态学杂志, 2009, 28(08): 1499-1504.
[7]	单国彬;金文标;林佶侃^1,4;邢新会. 变性梯度凝胶电泳在环境微生物生态学中的应用 [J]. 生态学杂志, 2006, 25(10): 1257-1264 .
[8]	夏汉平. 人工湿地处理污水的机理与效率 [J]. 生态学杂志, 2002, (4): 52-59.
[9]	胡尚勤. 蒜辣素对微生态制剂菌群影响的研究 [J]. 生态学杂志, 2002, (3): 18-20.