不同水生植物残体分解过程中真菌群落结构

生态学杂志

不同水生植物残体分解过程中真菌群落结构

郑九文^1,2,邢鹏^2**,余多慰¹,郭远^2,3

（¹南京师范大学生命科学学院，南京 210046; ²中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室，南京 210008; ³中国药科大学生命科学学院，南京 210009）

出版日期:2013-02-10 发布日期:2013-02-10

Fungal community structure during the decomposition of various aquatic plant litters.

ZHENG Jiu-wen^1,2, XING Peng^2**, YU Duo-wei¹, GUO Yuan^2,3

(¹College of Life Science, Nanjing Normal University, Nanjing 210046, China; ² State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing 210008, China; ³College of Life Science, China Pharmaceutical University, Nanjing 210009, China)

Online:2013-02-10 Published:2013-02-10

摘要/Abstract

摘要： 水生植物是浅水湖泊主要的初级生产者，其分解和矿化过程对于水体营养物质循环具有重要影响。真菌是生态系统中植物残体的主要分解者之一，其在不同水生植物残体分解过程中的群落组成和功能目前尚缺乏系统的研究。本研究通过在完整的沉积物和上覆水体系中添加不同的植物残体，在确定适合的扩增真菌18S rRNA基因特异性引物的基础上，通过构建克隆文库以及序列分析，研究了水体和表层沉积物在不同水生植物残体分解过程中的真菌群落组成。结果显示，引物对nu-SSU0817/nu-SSU-1536和nu-SSU-0817/nu-SSU-1196更适合扩增真菌18S rRNA基因；水体中的真菌类群主要是担子菌(Basidiomycota)，而表层沉积物中主要是壶菌(Chytridiomycota)。统计分析显示，水体和表层沉积物中真菌群落结构存在明显差异。本研究有助于进一步认识真菌在水生植物残体分解过程中的功能和生态位分化。

关键词: 土壤肥力长期试验, 土壤肥力变化

Abstract: Aquatic plants are the main primary producers in shallow lake, and their decomposition and mineralization have significant effects on the nutrient cycle in lake ecosystems. Fungi are one of the major decomposers in natural environments, but little is known about the composition and functions of fungal community during the decomposition of various aquatic plant litters. In this paper, various types of aquatic plant residues were separately added into undisturbed lake sediments and their upper water to study the characteristics of fungal community structure during the decomposition of these residues, based on the selection of suitable primers that can specifically amplify fungal 18S rRNA gene and on the construction of clone libraries and sequencing. The results showed that the primer pairs nu-SSU-0817/nu-SSU-1536 and nu-SSU-0817/nu-SSU-1196 were suitable to amplify the fungal 18S rRNA gene. Basidiomycota was the most common fungal taxa in water column, and Chytridiomycota was the most common one in surface sediments. Statistical analysis showed that there existed obvious differences in the fungal community structure in water column and surface sediments. This study would give further insight into the functions and niche differentiation of fungi during the decomposition of aquatic plant litters.

郑九文1,2,邢鹏2**,余多慰1,郭远2,3. 不同水生植物残体分解过程中真菌群落结构[J]. 生态学杂志.

ZHENG Jiu-wen1,2, XING Peng2**, YU Duo-wei1, GUO Yuan2,3. Fungal community structure during the decomposition of various aquatic plant litters.[J]. cje.