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生态学杂志 ›› 2010, Vol. 29 ›› Issue (11): 2192-2198.

• 研究报告 • 上一篇    下一篇

双酚A对斑马鱼肝脏和性腺的作用

曹 娜,魏 华**,吴陵广,吴婷婷,李国鹏   

  1. 上海海洋大学水产与生命学院,上海 201306
  • 出版日期:2010-11-08 发布日期:2010-11-08

Effects of bisphenol A on zebrafish (Danio rerio) liver and gonad.

CAO Na, WEI Hua, WU Ling-guang, WU Ting-ting, LI Guo-peng   

  1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
  • Online:2010-11-08 Published:2010-11-08

摘要: 为了阐明内分泌干扰物双酚A(BPA)对水生动物的毒理作用机制, 本文研究了BPA对斑马鱼急性毒性及组织学损伤作用。结果表明:BPA对斑马鱼具有急性毒性效应,96 h半致死浓度为6.3 mg·L-1,96 h暴露对斑马鱼的肝脏产生了浓度依赖性损伤;在高浓度组,精巢结构损伤,卵泡萎缩严重(闭锁率35%);在0.2 mg·L-1亚致死浓度BPA组中,暴露96 h后,斑马鱼肝脏受到轻微损伤,性腺组织结构没有受到明显损伤,卵巢中处于第Ⅲ时相晚期和第Ⅳ时相配子数比率增加,单个卵母细胞直径增加(P<0.05);在2 mg·L-1亚致死浓度BPA组中,暴露96 h,斑马鱼的肝脏组织受到了一定损伤,细胞核萎缩变形,细胞肿胀,核固缩,性腺组织受损不明显,卵巢中第Ⅲ时相晚期和第Ⅳ时相配子数比率有所增加;在6.1 mg·L-1BPA组中,暴露96 h,斑马鱼肝脏产生了极大的损伤,细胞空泡化,大面积的组织坏死,空洞产生,精巢组织结构受到一定损伤,卵巢中发现大量细胞萎缩现象;BPA对斑马鱼肝脏具有靶器官毒性,对性腺作用值得进一步研究。

关键词: 桃一点叶蝉, 草间小黑蛛, 三维空间格局, 时序动态

Abstract: Aimed to clarify the toxicological mechanism of bisphenol A (BPA) in aquatic animal, the toxicity and histological damage of BPA on zebrafish (Danio rerio) were studied. BPA had acute toxicity on zebrafish. The 96 h LC50of BPA was 6.3 mg·L-1. After 96 h exposure to BPA, the liver damage  were observed in concentration-dependent way. In high BPA group, the testis structure was damaged, and the atresia rate of ovarian follicle was 35%. In 0.2 mg BPA·L-1 group, liver was slightly damaged, gonad had no morphological change, the rate of gametes number in late phase Ⅲ to that in phase Ⅳ in ovary had some increase, and oocyte size increased significantly (P<0.05). In 2 mg BPA·L-1group, liver was damaged, hepatocyte cloudy swelling and pyknosis were observed, and the rate of gametes number in late phase Ⅲ to that in phase Ⅳ in ovary was slightly increased. In 6.1 mg BPA·L-1 group, liver was badly damaged with cell vacuolization, necrosis, and cavity, testis structure was subject to certain damage, pyknotic nuclei were also observed, and atresia of overian follicle increased significantly. The results indicated that liver was the target organ of BPA. It remains to be further studied whether gonad damage is caused by toxicological influence or by endocrine disrupting.

Key words: Erythroneura sudra, Erigonidum graminicola, Three-dimentional distribution pattern, Time series dynamics