关键词: 微卫星DNA, 分离, 富集

Abstract: Landscape fragmentation and habitat loss are widely recognized as the major threats to the biodiversity decrease and species extinction. In order to widen wildlife’s living area, ecological corridors have been built between fragmented habitats, which can not only stimulate gene exchange, but also improve species viability in fragmented habitats. Chongming Island, as an isolated deposition sand island of Shanghai, is significantly threatened by the decrease and fragmentation of forest patches. As a result, a great number of terrestrial animals whose living depends on forest habitats are tended to be extinct. In this paper, we chose hedgehog ( Erinaceus europaeus ), the first class protected animal, as the target species. Based on the analysis of its living behavior and matrix’s spatial distribution, this paper adopted the least-cost model and Geographic Information System (GIS) to help finding the best corridor between the living habitats of  E. europaeus . It was found that this method could provide a scientific basis for the ecological corridors’ planning and construction with the purpose of terrestrial wild animal conservation.

Key words: Microsatellite DNA, Isolation, Enrichment