中药提取物抑制油田微生物产硫化氢活性

doi:10.13292/j.1000-4890.202606.034

生态学杂志 ›› 2026, Vol. 45 ›› Issue (6): 2023-2031.doi: 10.13292/j.1000-4890.202606.034

中药提取物抑制油田微生物产硫化氢活性

杨春璐¹,杨宇祥^1,2,刘慧杰^1,2,李莹莹^1,2,吕雅清^1,2,赵金龙³,韩斯琴²,张颖²,史荣久^2*,夏瑛婕¹

（¹辽宁大学环境学院，沈阳 110036； ²中国科学院沈阳应用生态研究所，沈阳 110016； ³中国科学院金属研究所，沈阳 110016）

出版日期:2026-06-10 发布日期:2026-12-01

Chinese medicine extracts inhibit hydrogen sulfide-producing activities of oilfield microorganisms.

YANG Chunlu¹, YANG Yuxiang^1,2, LIU Huijie^1,2, LI Yingying^1,2, LYU Yaqing^1,2, ZHAO Jinlong³, HAN Siqin², ZHANG Ying², SHI Rongjiu^2*, XIA Yingjie¹

(¹School of Environment, Liaoning University, Shenyang 110036, China; ²Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ³Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China).

Online:2026-06-10 Published:2026-12-01

摘要/Abstract

摘要： 油田环境中微生物生长代谢产生硫化氢会引发油田酸败和腐蚀等生产及环境问题，但现役杀菌剂存在环境安全及耐药性隐患。为寻求环境友好型抑菌策略，以油田来源的多种硫化氢产生菌为受试对象，探索了艾叶、土黄连、莴苣、亚麻籽、茵陈、苍耳6种中药乙醇提取物的抑菌效果，另评估了土黄连、艾叶提取物与亚硝酸盐复配的效果。结果表明，初始浓度为5 mg·mL^-1的艾叶提取物、土黄连提取物均在15天内完全抑制了迈氏热厌氧杆状菌（Thermoanaerobacter mathranii）S1菌株、嗜铁温暖微菌（Tepidimicrobium ferriphilum）F1菌株和甘油穆尔氏菌（Moorella glycerini）A24菌株的产硫化氢活性。其他提取物部分有效或无效。土黄连、艾叶提取物分别与亚硝酸盐复配对菌株S1、A24、F1活性呈协同抑制效应，与单用相比，提取物用量可分别降低了75%、70%、80%和75%、75%、80%。土黄连提取物、艾叶提取物单用或其与亚硝酸盐复配具有防控油田微生物酸败的应用潜力。

关键词: 硫化氢产生菌, 中药提取物, 杀菌剂, 油田, 微生物酸败

Abstract: The microbial growth and metabolism in oilfields has been demonstrated to result in the production of hydrogen sulfide (H₂S), inducing a range of production and environmental challenges, including souring and corrosion. However, available biocides pose risks to environmental safety and resistance development. To explore eco-friendly antibacterial strategies, the inhibitory effects of ethanol extracts from six Chinese medicinal plants (Artemisia argyi, Berberis julianae, Lactuca sativa, Linum usitatissimum, Artemisia capillaris, and Xanthium strumarium) were evaluated against multiple H₂S-producing bacterial species isolated from oilfield settings. Furthermore, the combined effects of B. julianae and A. argyi extract with nitrite were investigated. Results showed that A. argyi extract and B. julianae extract, at an initial concentration of 5 mg·mL^-1, completely suppressed the H₂S- production activity of Thermoanaerobacter mathranii strain S1, Tepidimicrobium ferriphilum strain F1, and Moorella glycerini strain A24 within a 15-day period. In contrast, other extracts showed no effects on all the strains. The combination of B. julianae extract or A. argyi extract with nitrite produced a synergistic inhibitory effect on strains S1, A24, and F1. These combinations resulted in a more than 70% reduction in the required extract doses when compared with the use of individual agents. The utilization of A. argyi and B. julianae extract, either individually or in conjunction with nitrite, possesses the potential for environmentally sustainable microbial souring control in oilfield operations.

Key words: sulfide hydrogen-producing bacteria, Chinese medicine extract, biocide, oilfield, microbial souring

杨春璐, 杨宇祥, 刘慧杰, 李莹莹, 吕雅清, 赵金龙, 韩斯琴, 张颖, 史荣久, 夏瑛婕. 中药提取物抑制油田微生物产硫化氢活性[J]. 生态学杂志, 2026, 45(6): 2023-2031.

YANG Chunlu, YANG Yuxiang, LIU Huijie, LI Yingying, LYU Yaqing, ZHAO Jinlong, HAN Siqin, ZHANG Ying, SHI Rongjiu, XIA Yingjie. Chinese medicine extracts inhibit hydrogen sulfide-producing activities of oilfield microorganisms.[J]. Chinese Journal of Ecology, 2026, 45(6): 2023-2031.

中药提取物抑制油田微生物产硫化氢活性

Chinese medicine extracts inhibit hydrogen sulfide-producing activities of oilfield microorganisms.

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