盐胁迫下盐地碱蓬和芦苇对氮磷营养盐的吸收动力学特征

生态学杂志 ›› 2025, Vol. 44 ›› Issue (6): 1937-1944.

盐胁迫下盐地碱蓬和芦苇对氮磷营养盐的吸收动力学特征

黄颖琳¹,孙晓瑄¹,管博^1*,崔雯慧¹,王光美²,刘晓玲²,于君宝^1*

（¹鲁东大学滨海生态高等研究院，山东烟台 264025； ²中国科学院海岸带环境过程与生态修复重点实验室，中国科学院烟台海岸带研究所，山东烟台 264003)

出版日期:2025-06-10 发布日期:2025-06-06

The kinetics of nitrogen and phosphorus uptake by Suaeda salsa and Phragmites australis under salt stress.

HUANG Yinglin¹, SUN Xiaoxuan¹, GUAN Bo^1*, CUI Wenhui¹, WANG Guangmei², LIU Xiaoling², YU Junbao^1*

(¹Institute of Advanced Studies in Coastal Ecology, Ludong University, Yantai 264025, Shandong, China; ²Key Laboratory of Coastal Zone Environmental Processes and Ecological Restoration, Chinese Academy of Sciences, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong, China).

Online:2025-06-10 Published:2025-06-06

摘要/Abstract

摘要： 为了解盐胁迫对盐地碱蓬（Suaeda salsa）和芦苇（Phragmites australis）氮磷吸收动力学特征的影响，本研究采用营养耗竭法，设置不同NaCl浓度（盐地碱蓬：0和1.5%；芦苇：0和1%）和氮磷浓度（NH₄⁺-N：0.25、1、5、10、15 mmol·L^-1；H₂PO₄^--P：0.25、0.5、1、2、5、10 mmol·L^-1），在0、1、2、3、5、7、9、12 h测量溶液中离子浓度，获得盐地碱蓬和芦苇对氮磷的吸收速率和吸收动力学参数。结果表明：盐地碱蓬对NH₄⁺-N、H₂PO₄^--P的吸收均可用Michaelis-Menten方程描述，1.5% NaCl胁迫下盐地碱蓬对NH₄⁺-N的吸收曲线趋于线性，无盐胁迫下逐渐趋于饱和；1.5% NaCl胁迫对盐地碱蓬吸收NH₄⁺-N和H₂PO₄^--P具有抑制作用；盐地碱蓬在1.5% NaCl胁迫下吸收NH₄⁺-N的最大吸收速率（V_max）比无盐胁迫处理提高157.43%，米氏常数值（K_m）提高1542.99%，NH₄⁺-N在根系中的流速（α）降低84.35%；吸收H₂PO₄^--P的V_max比无盐胁迫处理降低10.18%，K_m值提高23.92%，α值下降27.84%。对芦苇而言，NH₄⁺-N和H₂PO₄^--P吸收动力学特征均可用Michaelis-Menten方程描述，吸收曲线均符合饱和动力学特征；1% NaCl胁迫对芦苇吸收NH₄⁺-N和H₂PO₄^--P具有促进作用，芦苇在盐胁迫下吸收NH₄⁺-N的V_max比无盐胁迫处理提高16.65%，K_m值降低53.02%，α值提高147.02%；吸收H₂PO₄^--P的V_max提高166.77%，K_m值降低28.35%，α值提高278.57%。盐地碱蓬与芦苇在盐胁迫下展现出不同的氮磷吸收特征，其差异可能是不同植物的养分吸收策略所致，该结果可为滨海湿地植被恢复和净化功能研究提供一定的理论依据。

关键词: 盐地碱蓬, 芦苇, 动力学特征, 氮, 磷, 盐胁迫

Abstract: To understand the effects of salinity on nitrogen and phosphorus uptake of Suaeda salsa and Phragmites australis, the uptake characteristics of NH₄⁺-N and H₂PO₄^--P of both species under salt stress were investigated by depletion method. Two levels of NaCl (S. salsa: 0 and 1.5%, P. australis: 0 and 1%), five levels of NH₄⁺-N (0.25, 1, 5, 10, and 15 mmol·L^-1), and six levels of H₂PO₄^--P (0.25, 0.5, 1, 2, 5, and 10 mmol·L^-1) were established. The concentration of the solution was measured at 0, 1, 2, 3, 5, 7, 9 and 12 h to obtain the uptake rate and kinetic characteristics of nitrogen and phosphorus by S. salsa and P. australis. The results showed that the uptake of NH₄⁺-N and H₂PO₄^--P by S. salsa fitted the Michaelis-Menten equation. Uptake kinetics of NH₄⁺-N by S. salsa showed a linear response at 1.5% NaCl, but saturated at 0 NaCl. 1.5% NaCl inhibited the uptake of NH₄⁺-N and H₂PO₄^--P by S. salsa. Compared to the 0 NaCl treatment, the maximum absorption rate (V_max) and the Michaelis constant value (K_m) of NH₄⁺-N uptake by S. salsa increased by 157.43% and 1542.99% at 1.5% NaCl, respectively, while the flow rate of NH₄⁺-N in root (α) decreased by 84.35%. Compared to the 0 NaCl treatment, the V_max and α of H₂PO₄^--P decreased by 10.18% and 27.84% at 1.5 % NaCl, and K_m increased by 23.92%. For P. australis, the uptake kinetics of NH₄⁺-N and H₂PO₄^--P could be described by Michaelis-Menten equation. The absorption curves were consistent with the saturation kinetics characteristics. 1% NaCl promoted the uptake of NH₄⁺-N and H₂PO₄^--P. Compared to the 0 NaCl treatment, the V_max of NH₄⁺-N uptake by P. australis increased by 16.65%, K_m decreased by 53.02%, α increased by 147.02% at 1% NaCl. The V_max of H₂PO₄^--P uptake increased by 166.77%, K_m decreased by 28.35%, and α increased by 278.57% at 1% NaCl. Collectively, our findings demonstrate that the uptake characteristics of nitrogen and phosphorus by S. salsa and P. australis under salt stress differ significantly. This discrepancy may be attributed to their distinct nutrient acquisition strategies. Our results provide a theoretical foundation for investigating vegetation restoration and purification functions in coastal wetlands.

Key words: Suaeda salsa, Phragmites australis, kinetic characteristics, nitrogen, phosphorus, salinity

黄颖琳, 孙晓瑄, 管博, 崔雯慧, 王光美, 刘晓玲, 于君宝. 盐胁迫下盐地碱蓬和芦苇对氮磷营养盐的吸收动力学特征[J]. 生态学杂志, 2025, 44(6): 1937-1944.

HUANG Yinglin, SUN Xiaoxuan, GUAN Bo, CUI Wenhui, WANG Guangmei, LIU Xiaoling, YU Junbao. The kinetics of nitrogen and phosphorus uptake by Suaeda salsa and Phragmites australis under salt stress.[J]. Chinese Journal of Ecology, 2025, 44(6): 1937-1944.

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