氮添加对大兴安岭泥炭地典型植物叶片叶绿素和养分含量的影响

生态学杂志 ›› 2025, Vol. 44 ›› Issue (5): 1456-1464.

氮添加对大兴安岭泥炭地典型植物叶片叶绿素和养分含量的影响

冯欢欢^1,2,高思齐^1,3,4,高晋丽¹,杜宇¹,刘桢迪^1,3,陈宁¹,宋艳宇^1*

（¹中国科学院东北地理与农业生态研究所湿地生态与环境重点实验室，长春 130102； ²吉林农业大学资源与环境学院，长春 130118； ³中国科学院大学资源与环境学院，北京 100049； ⁴中国科学院生态环境研究中心中国科学院城市与区域生态国家重点实验室，北京 100085）

出版日期:2025-06-10 发布日期:2025-05-08

Effect of nitrogen addition on leaf chlorophyll and nutrient content of typical plant species in peatland of the Great Hing’an Mountains.

FENG Huanhuan^1,2, GAO Siqi^1,3,4, GAO Jinli¹, DU Yu¹, LIU Zhendi^1,3, CHEN Ning¹, SONG Yanyu^1*

(¹Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; ²College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China; ³College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; ⁴State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China).

Online:2025-06-10 Published:2025-05-08

摘要/Abstract

摘要： 叶绿素作为植物光合作用的主要色素，其含量与土壤氮营养环境密切相关，对植物的生长和养分吸收具有重要影响。为探讨氮添加对大兴安岭多年冻土区泥炭地植物生长和养分特征的影响，基于长期氮添加试验（N1：6 g·m^-2·a^-1；N2：12 g·m^-2·a^-1；N3：24 g·m^-2·a^-1），分析泥炭地典型植物柴桦、甸杜、笃斯越橘叶片叶绿素和碳、氮、磷含量及其化学计量比对氮添加的响应规律。结果表明：氮添加提高了泥炭地柴桦、甸杜、笃斯越橘叶片的叶绿素含量，且随氮添加浓度的增加呈现先增加后降低的变化趋势；中浓度氮添加提高了柴桦和甸杜叶片的总碳含量，但不同浓度氮添加对笃斯越橘叶片总碳含量无显著影响；氮添加显著提高了柴桦和甸杜叶片的总氮含量；氮添加降低了3种植物叶片的总磷含量及C/N，提高了植物叶片的N/P。相关性分析表明：柴桦、甸杜和笃斯越橘叶片叶绿素含量均与总氮含量呈显著正相关，与C/N呈显著负相关；甸杜叶片叶绿素含量与C/P呈显著正相关，与N/P呈极显著正相关；笃斯越橘叶片叶绿素含量与总氮、C/P、N/P呈极显著正相关，与总磷、C/N呈极显著负相关。研究表明：大兴安岭多年冻土泥炭地植物生长受氮限制，氮添加可通过提高冻土区泥炭地植物叶片叶绿素含量提高其光合能力，并促进植物对氮的吸收和累积，改变其生态化学计量特征。

关键词: 氮营养, 冻土区泥炭地, 叶绿素, 氮限制, 化学计量比

Abstract: As the main pigment for plant photosynthesis, chlorophyll content is closely related to soil nitrogen availability, with consequence on plant growth and nutrient absorption. We conducted a long-term nitrogen addition experiment (N1: 6 g·m^-2·a^-1; N2: 12 g·m^-2·a^-1; N3: 24 g·m^-2·a^-1) in the permafrost region of the Great Hing’an Mountains to investigate the effects of nitrogen addition on plant growth and nutrient characteristics of peatland. The response patterns of leaf chlorophyll content and carbon, nitrogen, and phosphorus contents and their stoichiometric ratios to nitrogen addition were analyzed for typical peatland plants, including Betula fruticosa, Chamaedaphne calyculata, and Vaccinium uliginosum. The results showed that nitrogen addition increased leaf chlorophyll contents of B. fruticosa, C. calyculata, and V. uliginosum. Chlorophyll contents showed a trend of first increasing and then decreasing with increasing nitrogen addition levels. Mid-level of nitrogen addition significantly increased total leaf carbon contents of B. fruticosa and C. calyculata, but did not affect that of V. uliginosum. Nitrogen addition significantly increased total nitrogen contents of B. fruticosa and C. calyculata leaves, while simultaneously reduced the total phosphorus content and C/N ratio, and increased foliar N/P ratio of all the three species. Leaf chlorophyll contents of B. fruticosa, C. calyculata and V. uliginosum were significantly positively correlated with total leaf nitrogen content, whereas negatively correlated leaf C/N ratio. The chlorophyll content of C. calyculata was significantly positively correlated with C/P and N/P. In contrast, the chlorophyll content of V. uliginosum leaves was significantly positively correlated with total nitrogen, C/P, and N/P, and significantly negatively correlated with total phosphorus and C/N. Our results suggested that the growth of peatland plants was limited by nitrogen. Nitrogen addition can improve the photosynthetic capacity of peatland plants in permafrost regions, promote nitrogen absorption and accumulation, and change ecological stoichiometry.

Key words: nitrogen nutrition, permafrost peatland, chlorophyll, nitrogen limitation, stoichiometric ratio

冯欢欢, 高思齐, 高晋丽, 杜宇, 刘桢迪, 陈宁, 宋艳宇. 氮添加对大兴安岭泥炭地典型植物叶片叶绿素和养分含量的影响[J]. 生态学杂志, 2025, 44(5): 1456-1464.

FENG Huanhuan, GAO Siqi, GAO Jinli, DU Yu, LIU Zhendi, CHEN Ning, SONG Yanyu. Effect of nitrogen addition on leaf chlorophyll and nutrient content of typical plant species in peatland of the Great Hing’an Mountains.[J]. Chinese Journal of Ecology, 2025, 44(5): 1456-1464.

[1]	王强, 马晓云, 李钰, 冯梦楠, 曹冬梅. 植物生长调节剂促进观赏凤梨分蘖有效成分筛选及机制 [J]. 生态学杂志, 2025, 44(4): 1202-1211.
[2]	何金松, 吴小红, 王卫, 闫文德. 不同水分管理对亚热带稻田土壤化学计量比和水稻产量的影响 [J]. 生态学杂志, 2025, 44(3): 920-929.
[3]	伍盈盈, 肖晶. 基于生源要素结构变化驱动的喀斯特水库富营养化特征辨识 [J]. 生态学杂志, 2025, 44(2): 556-565.
[4]	陈庆达, 刁思月, 胡菁格, 肇同斌, 祝成瑶, 李秀芬. 红松幼苗光合特性对低温和光照变化的响应 [J]. 生态学杂志, 2024, 43(9): 2623-2631.
[5]	余顺平, 熊于斌, 杨娅, 俞海冰, 胡良松, 黄琳, 汤利. 化肥减量配施不同有机肥的植烟土壤-微生物碳氮磷生态化学计量特征 [J]. 生态学杂志, 2024, 43(7): 2150-2157.
[6]	高浩英, 付双嘉, 宋世钰, Samra Batool, 王丽霞. 川西亚高山云杉天然林和人工林土壤酶化学计量特征比较 [J]. 生态学杂志, 2024, 43(6): 1531-1539.
[7]	其曼古丽·帕拉提, 刘丹, 毛军, 乔福生, 王长庭. 不同退化程度高寒草地土壤碳氮磷含量及生态化学计量特征 [J]. 生态学杂志, 2024, 43(6): 1612-1620.
[8]	陈晓楠, 伊力努尔·艾力, 高文礼, 王海鸥, 麦格皮热提古丽·达吾提, 马晓东. 盐胁迫下接种丛枝菌根真菌对两种典型荒漠河岸林植-物幼苗生长和叶绿素荧光特性的影响 [J]. 生态学杂志, 2024, 43(5): 1333-1340.
[9]	王世彤, 宋帅帅, 李杰华, 杨腾, 何艺琴, 魏新增, 江明喜. 极小种群野生植物黄梅秤锤树的光合生理特性 [J]. 生态学杂志, 2024, 43(3): 701-708.
[10]	周欢, 韦如萍, 李吉跃, 苏艳, 胡德活, 郑会全. 光照强度对乐昌含笑幼苗生长及光合特性的影响 [J]. 生态学杂志, 2024, 43(3): 709-715.
[11]	夏烨, 陈敬华, 王绍强, 孙雷刚, 陈斌, 陈世亮, 王钦艺, 赵紫祺. 基于多源遥感信息的河北省陆地植被生产力模拟分析 [J]. 生态学杂志, 2024, 43(12): 3735-3745.
[12]	李慧超, 潘红丽, 冯秋红, 马文宝, 邓东周. 凋落叶输入量对川西亚高山不同密度云杉人工林土壤酶活性及其化学计量比的影响 [J]. 生态学杂志, 2024, 43(10): 2967-2978.
[13]	冷寒冰, 苏鸣, 张春英. 锦绣杜鹃对不同光环境的光合特性响应及适应机制 [J]. 生态学杂志, 2023, 42(8): 1793-1801.
[14]	申景昕, 范少辉, 刘广路, 魏松坡. 毛竹林带采伐宽度对土壤碳氮磷含量及计量比的影响 [J]. 生态学杂志, 2023, 42(8): 1851-1857.
[15]	杨永娥, 张晓煜, 冯蕊, 李芳红, 梁小娟, 马梦瑶, 范锦龙. 遮光对澳洲青苹叶片光合特性的影响 [J]. 生态学杂志, 2023, 42(7): 1661-1669.