欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志 ›› 2020, Vol. 39 ›› Issue (9): 2921-2929.doi: 10.13292/j.1000-4890.202009.012

• 研究报告 • 上一篇    下一篇

连栽杉木根际土壤镰刀菌属真菌群落变化规律

罗扬1,刘书影1,周柳婷1,白莹1,陈军1,林文雄1,吴则焰1,2,3*   

  1. 1福建农林大学生命科学学院, 福州 350002;2作物生态与分子生理学福建省高校重点实验室, 福州 350002; 3福建省农业生态过程与安全监控重点实验室, 福州 350002)
  • 出版日期:2020-09-10 发布日期:2021-03-10

Fusarium community variation in rhizosphere soil of continuous planting Chinese fir plantation.

LUO Yang1, LIU Shu-ying1, ZHOU Liu-ting1, BAI Ying1, CHEN Jun1, LIN Wen-xiong1, WU Ze-yan1,2,3*   

  1. (1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3Fujian Provincial Key Laboratory of Agro-ecological Processing and Safety Monitoring, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China).
  • Online:2020-09-10 Published:2021-03-10

摘要: 为探讨连栽对杉木(Cunninghamia lanceolata (Lamb.) Hook.)根际土壤镰刀菌属真菌多样性的影响,本研究应用DGGE技术研究连栽杉木根际土壤镰刀菌属(Fusarium)真菌在一代杉木人工林(first rotation Chinese fir plantation, FCP)、二代杉木人工林(second rotation Chinese fir plantation, SCP)与三代杉木人工林(third rotation  Chinese fir plantation, TCP)的组成及含量变化,并结合qRT-PCR技术测定杉木根际土壤病原菌尖孢镰刀菌(Fusarium oxysporum)的绝对含量变化,从而揭示杉木连栽障碍土壤微生态失衡现象。DGGE结果表明,多代连栽后杉木根际土壤镰刀菌属真菌组成及含量均发生显著变化,优势菌尖孢镰刀菌含量随着栽植代数的增加显著上升。主成分分析能较好区分FCP、SCP与TCP根际土壤镰刀菌群落特征。聚类分析结果表明,TCP土壤镰刀属结构组成及百分含量与FCP、SCP差异显著。多样性分析结果显示,连栽杉木土壤镰刀菌多样性与丰富度均呈现FCP<SCP<TCP。qRT-PCR分析表明,FCP、SCP与TCP根际土壤尖孢镰刀菌的绝对含量随栽植代数增加不断上升,进一步验证了DGGE结果。由此推测,杉木连栽障碍与根际土壤微生态失衡密切相关,其中尖孢镰刀菌含量的上升是重要因素之一。

 

关键词: 杉木, 根际土壤微生物, 镰刀菌群落结构, 变形梯度凝胶电泳, 实时荧光定量PCR

Abstract: We examined the effects of continuous planting on fungi diversity of Fusarium in the rhizosphere soil of Chinese fir plantation. We analyzed the composition and content of Fusariumin soils of the first (FCP), second (SCP), and third (TCP) generation of Chinese fir plantations using DGGE technology, and measured the absolute content of Fusarium oxysporum in rhizosphere soil using qRT-PCR technique. DGGE results showed that the composition and content of Fusarium in the rhizosphere soil changed significantly after multiple generations of planting, with the content of F. oxysporum being increased significantly with increasing generation. Through principal component analysis, Fusarium community characteristics of FCP, SCP and TCP rhizosphere soil were well distinguished. The results of cluster analysis showed that the structural composition and percentage of Fusarium in the TCP soil were significantly different from those of FCP and SCP. The results of diversity analysis showed that the diversity and richness ofFusariumdecreased following a sequence of FCP<SCP<TCP. The results of qRT-PCR analysis showed that the absolute content of F. oxysporum in the rhizosphere soil of FCP, SCP and TCP increased continuously with increasing planting generation, which further verified the DGGE results. Ourresults suggest that the continuous planting obstacles of Chinese fir are closely related to the micro-ecological imbalance in rhizosphere soil, with the increase ofF. oxysporum content being one of the important factors.

Key words: Chinese fir, rhizosphere soil microbe, Fusarium community structure, DGGE, qRT-PCR.