Abstract: Chlorophyll-a concentration is an important parameter reflecting the degree of eutrophication. Based on the field data and MODIS remote sensing data, this study achieved the inversion models of chlorophylla concentration in nine lakes in Yunnan Province. Firstly, the optimum models of each lake in different seasons were established by the combination of the land surface radiation of different bands of MODIS with the measured chlorophyll-a concentration in 2010-2012. Secondly, the inversion models of the nine lakes were validated by using the measured chlorophyll-a concentration from 2013 to 2014. Then, the spatial distributions of chlorophylla concentration in nine lakes were analyzed. The spatial distribution of inversed chlorophyll-a concentration was in accordance with the professional judgment of experts, indicating the inverse models were reasonable. The chlorophyll-a concentrations in Dianchi Lake, Erhai Lake, Chenghai Lake, Qilu Lake, Xingyun Lake, Yilong Lake and Yangzong Lake decreased from the border to the center of lake. However, the chlorophyll-a concentrations in Fuxian Lake and Lugu Lake were higher in the center than in the border of lake. During the period 2010-2014, the concentrations of chlorophyll-a of Dianchi Lake, Erhai Lake, Xingyun Lake and Yilong Lake had larger fluctuation, while those of Fuxian Lake, Chenghai Lake, Qilu Lake and Lugu Lake were relatively stable, and that of Yangzong Lake showed a decreasing trend.