Land use data of Anqiu City of Shandong Province in 1995, 2000, 2005 and 2010 were used to analyze the dynamic changes of ecologically used land by a combined approach including dynamic degree, transition probability matrix, and spatial autocorrelation analysis. Our objective is to provide a decisionmaking reference for the sustainable development and environmental protection during the construction of a new countryside of Anqiu City. The results indicated that the area ratio of ecologically used land decreased from 14.04% to 13.31% from 1995 to 2010 due to the decreased grassland and water areas and increased forest and unused land areas. In contrast, the construction land continuously increased and the farmland continuously decreased during this period. In the three periods, unstable patches had low area rates of 1.39%, 2.21% and 3.13%, respectively. The area and number ratios of ecologically used land of the unstable patches were relatively low, being 31.09%, 43.21%, 20.52% and 28.24%, 34.06%, 11.28%, respectively. The spatial stability of ecologically used land was relatively low and showed a downward trend. Moran index analysis indicated that the transition of other lands to ecologically used land was spatially clustered, while the transition to nonecologically used land dispersed or was at random. Overall, the ecologically used land moved in the direction of shrinking and the nonecologically used land in the direction of expanding.