Abstract: From July 2010 to June 2011, twelve soil samples were monthly collected from 0-10, 10-20 and 20-30 cm depths of Lonicera japonica rhizosphere in Anguo City, Hebei Province of China, with the correlations between arbuscular mycorrhizal (AM) fungi, glomalin, and soil environment studied by using canonical correlation analysis (CCA). It was found that the correlation between soil layer and vesicular colonization could best explain the canonical correlation of AM fungi and soil environment (r=0.89). The canonical correlation between AM fungi and soil environment was mainly presented in the correlation between the total glomalin concentration and the organic C content in soil (r=0.87); whereas the canonical correlation between AM fungi and glomalin was mainly reflected as the correlation between spore density and total glomalin (r=0.85). The further analysis indicated that soil layer had significant negative correlations with vesicular colonization, spore density, and total glomalin. The soil total nitrogen was significantly and positively correlated with the colonization of exogenous hyphae, but negatively correlated with the colonization of arbuscular hyphae. In addition, both spore density and hyphae colonization had significant positive correlations with glomalin, and total glomalin was significantly positively correlated with vesicular colonization, indicating that glomalin could be generated from vesicles.