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不同间隔年限地黄土壤的自毒作用和酚酸类物质含量

杜家方1;尹文佳1;张重义1,2;侯健1;黄剑1;李娟1   

  1. 1河南农业大学中药材系, 郑州 450002; 2福建农林大学农业生态研究所, 福州 350002
  • 收稿日期:2008-06-02 修回日期:1900-01-01 出版日期:2009-03-10 发布日期:2009-03-10

Autotoxicity and phenolic acids content in soils with different planting interval years of Rehmannia glutinosa.

DU Jia-fang1;YIN Wen-jia1;ZHANG Zhong-yi1,2;HOU Jian1;HUANG Jian1;LI Juan1   

  1. 1Department of Chinese Herbal Medicine, Henan Agricultural University, Zhengzhou 450002, China;2Institute of Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2008-06-02 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10

摘要: 分别取2、4、6、8年前种植过地黄的土壤进行大田再植生物测试,并采用HPLC检测再植地黄土壤中与化感现象密切相关的5种酚酸(阿魏酸、对羟基苯甲酸、香草酸、香豆酸和丁香酸)的含量。结果表明:随着土壤间隔年限的增加,大田再植地黄的块根干质量、体积和叶片干质量依次增加,且从地黄苗期就开始表现出这种变化趋势;地黄土壤中5种酚酸的含量也依次降低, 间隔2、4、6、8年的再植地黄土壤中5种一元酚酸总含量分别为1.503、1.323、0.700和0.340 μg·g-1。间隔2、4、6、8年的再植地黄土壤水提液中阿魏酸、对羟基苯甲酸和香豆酸含量与地黄大田生物测试结果呈显著或极显著负相关。从整体看,地黄生育的前期和中期应当是地黄化感作用的重点时期。

关键词: 凋落量, 森林类型, 凋落组分, 年变化

Abstract: Field replanting bioassay was conducted on the soils having planted wi th Rehmannia glutinosa for 2, 4, 6, and 8 years. The soils were repl anted with R. glutinosa, and 5 phenolic acids (coumalic acid, 4-hydroxybenz oic acid, venillic acid, syringic acid and ferulic acid) closed related to allel opathetic effects in the replanted soils were analyzed by using high pressure li quid chromatography (HPLC). The results showed that on the soils with increasing planting interval years of R. glutinosa, the root tube dry mass and volume and the leaf dry mass of replanted R. glutinosa increased since seeding stag e then, while the contents of test five phenolic acids in the replanted soils de creased accordingly. The total contents of test five monohydric phenolic acids i n the replanted soils having been planted with R. glutinosa for 2, 4, 6, and 8 years was 1503, 1323, 0700, and 0340 μg·g-1, respectively, an d the content of ferulic acid, 4-hydroxybenzoic acid, and coumalic acid in soil water extract had significant negative correlation with filed planting bioassay results. As a whole, the early and middle growth stages of R. glutinosa sho uld be the crucial periods for the allelopathetic effects of R. glutinous.

Key words: Litterfall, Forest type, Litter components, Litter dynamics