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生态学杂志 ›› 2022, Vol. 41 ›› Issue (10): 1903-1908.doi: 10.13292/j.1000-4890.202209.023

• 研究报告 • 上一篇    下一篇

红江橙内生固氮菌生物多样性和种群结构

孙鹏,王锂韫,卢冬梅,袁红旭*   

  1. (岭南师范学院生命科学与技术学院, 广东湛江 524048)
  • 出版日期:2022-10-10 发布日期:2022-10-11

Diversity and community structure of endophytic diazotroph isolated from Hongjiang Orange.

SUN Peng, WANG Li-yun, LU Dong-mei, YUAN Hong-xu*   

  1. (Life Science and Technology School, Lingnan Normal University, Zhanjiang 524048, Guangdong, China).
  • Online:2022-10-10 Published:2022-10-11

摘要: 采用16S rRNA基因序列分析法对红江橙中分离的内生固氮菌进行分类鉴定,根据Shannon多样性指数、菌株的相对分离频率和相似性指数分析红江橙内生固氮菌的菌群分布及多样性,并比较患黄龙病植株与健株内生固氮菌菌群。结果表明:红江橙内生固氮菌属类丰富,从红江橙根、茎、叶中分离得到内生固氮菌72株,归属为3门4纲5目8科12属,其中芽孢杆菌属、短小杆菌属、泛菌属和伯克霍尔德菌属为红江橙内生固氮菌优势菌群,其相对分离频率分别为36.11%、13.89%、13.89%和9.72%。红江橙内生固氮菌总Shannon多样性指数(H)2.01,总均匀度指数(E)0.62,各部位间相似性指数分别为Cs(root-stem)=0.29、Cs(stem-leaf)=0.40、Cs(root-leaf)=0.20,根、茎、叶间内生固氮菌相似性较低,红江橙叶中内生固氮菌属类最丰富,有9个属,其次根部6个属,茎部仅分离到1个属。患黄龙病对红江橙内生固氮菌Shannon多样性指数(Hhealth=1.87、Hdisease=1.91)及均匀度指数(Ehealth=0.67、Edisease=0.72)影响小,患病后短小杆菌属(RFhealth=9.38%、RFdisease=17.5%)和泛菌属(RFhealth=21.88%、RFdisease=7.5%)两类优势内生固氮菌相对分离率变化较大。


关键词: 红江橙, 内生固氮菌, 种群多样性, 芽孢杆菌属, 短小杆菌属, 泛菌属

Abstract: Endophytic diazotrophs isolated from Hongjiang Orange were identified by 16S rRNA sequence analysis. The biodiversity and distribution of endophytic diazotroph in Hongjiang Orange were revealed by Shannon diversity index (H), relative frequency (RF) and similarity index. The differences of the distribution of endophytic diazotroph between the healthy plants and the diseased plants infected by citrus yellow shoot disease were compared. The results showed that the genera of endophytic diazotroph of Hongjiang Orange were abundant. A total of 72 strains were isolated from different tissues of Hongjiang Orange, which belonged to 3 phyla, 4 classes, 5 orders, 8 families, 12 genera. Bacillus spp., Curtobacterium spp., Pantoea spp. and Burkholderia spp. were the dominant endophytic diazotroph of Hongjiang Orange, with relative frequency of 36.11%, 13.89%, 13.89% and 9.72%, respectively. The total Shannon diversity index (H) of endophytic diazotroph was 2.01, and the total evenness index was 0.62. The similarity index between different parts of Hongjiang Orange was Cs(root-stem)=0.29, Cs(stem-leaf)=0.40, Cs(root-leaf)=0.20, respectively, indicating a low similarity of endophytic diazotroph in different parts. There were 9 genera of endophytic diazotroph in leaf, 6 genera in root, and only 1 genus in stem. There was little difference in Shannon diversity index (Hhealth=1.87 vs. Hdisease=1.91) and evenness index (Ehealth=0.67 vs. Edisease=0.72) between healthy plants and diseased plants, while the relative frequency of two dominant endophytic azotobacter Curtobacterium (RFhealth=9.38% vs. RFdisease=17.5%) and Pantoea (RFhealth=21.88% vs. RFdisease=7.5%) changed greatly.


Key words: Hongjiang Orange, endophytic diazotroph, community diversity, Bacillus spp., Curtobacterium spp., Pantoea spp.