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Table of Content

    10 March 2010, Volume 29 Issue 03
    Articles
    Effects of wind blow and sand burial on the seedling growth and photosynthetic a
    nd transpiration rates of desert plants.
    DIAO Ha-Lin, HE Yu-Hui, YUE An-Yang, ZHOU Rui-Lian
    2010, 29(03):  413-419. 
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    Aimed to understand the effects of wind blow and sand burial on the ph
    ysiological and ecological properties of desert plants, a field experiment with
    Caragana microphilla was conducted in Horqin Sand Land in 2006 and 2007. The
     wind blow experiment had five treatments, i.e., 0, 2, 4, 6, and 8 m·s-1
    of wind velocity, and the sand burial experiment also had five treatments, i.e.,
    〖JP2〗 none, light, moderate, heavy, and severe sand burial, with 0, 33%, 66%, 100%, a
    nd 133% of plant height sandburied. A continuous wind blow with a velocity of
    2 m·s-1 resulted in a slight decrease of photosynthetic rate and transpir
    ation rate, that of 6〖KG-*2〗-〖KG-*7〗8 m·s-1 increased the two rates o
    bviously, while a continuous wind blow with a velocity of 4 m·s-1 had no
    obvious effects on the photosynthetic and transpiration rates. Light sand burial
     promoted the stem, leaf, and root growth, and increased the biomass of C. mic
    rophilla, moderate sand burial only promoted the root growth and increased the
     biomass but restrained the height growth, while heavy and severe sand burial in
    jured the plant growth seriously, even resulted in plant death. As a dominant sa
    ndfixing plant, C. microphilla could adapt the wi
    nd blow and sand burial to a certain degree through regulating its growth rhythm
     and physiological characteristics, and thus, had strong capability to adapt w
    indsand environment. However, severe wind blow and sand burial could result in
     a severe injury to the plant, even in plant death. Therefore, C. microphilla sh
    ould be planted in the place without strong windsand activity when used for fi
    xing mobile sand.
    Relationships between wholeplant sap flux characteristics of Caragana interm edia and environmental factors in loess hill-gully region.
    2010, 29(03):  420-426. 
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    In order to characterize the water consumption by Caragana intermedia, an excellent windbreaking and sandfixing shrub species widely planted in Inner Mongolia, its wholeplant transpiration was monthly measured by using Granier’s thermal dissipation probe. Meanwhile, the environmental factors such as solar radiation around sample plant, air temperature, air relative humidity, and soil temperature were also measured. The wholeplant transpiration had a diurnal variation with four distinct phases, i.e., rapid ascending phase, stationary high phase, rapid descending phase, and stationary low phase. Except for October, in which the trunk sap flow had a marked decrease, there was no significant difference in the sap flow in other months. In May, July, and September, the variation of trunk sap flow lagged behind solar radiation, but advanced vapor pressure deficit. Solar radiation was the key factor affecting the transpiration. Under sudden precipitation, a rapid increase of the wholeplant sap flux was observed, but the increase lagged behind the peak time of the precipitation.
    Caloric values of dominant plant species in Xishuangbanna tropical seasonal rainforest.
    2010, 29(03):  427-433. 
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    Aimed to explore the change patterns of the gross caloric value (GCV) of dominant plant species in Xishuangbanna tropical seasonal rainforest, the GCV of different organs of 37 dominant species in different layers of the forest was measured by SDACM-Ⅲa caloric value analyzer in dry and rainy seasons, respect
    ively. The mean GCV of the 37 dominant species was 17.05-20.23 kJ·g-1, and the GCV of trees, lianes, shrubs, and herbages was 18.86-20.23, 17.97-19.76, 17.71-18.83, and 17.05-17.45 kJ·g-1, respectively. The mean GCV of the dominant species in different layers in both dry and rainy seasons decreased in the
     order of tree layer>liane interlayer>shrub layer>herbage layer. For the dominant species at the same layers, the mean GCV of their organs did not display signi
    ficant differences, except for the GCV between leaves and roots or branches. No significant differences in the mean GCV of the same species in different layers were observed between dry and rainy seasons. Also, there were no significant differences in the mean GCV of the dominant species in the same layers in dry and rainy seasons.
    Litter production and nutrient return of vegetations in subalpine timberline ecotone of west Sichuan, China.
    2010, 29(03):  434-438. 
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    In 2006-2008, investigations were made on the litter production and nutrient return of the vegetations in subalpine timberline ecotone of west Sichuan. The annual litter production of the vegetations was 389.83 kg·hm-2·a-1, among which, leaf, branch, and miscellany occupied72.2%, 17.9%, and 9.9%, respectively. The annual return of five main nutrients (N, P, K, Ca, and Mg) from the litters was 1582 kg·hm-2·a-1 in total, with the sequence of Ca>N>K>Mg>P, and 87.4%, 7.0%, and 5.6% of the return came from leaf, branch, and miscellany, respectively. The monthly dynamics of different litter components and their nutrient return all showed onepeak, with the maximum in September for leaf and in October for branch and miscellany. The seasonal dynamics of the nutrient contents in litters differed with litter components and nutrient kinds. In leaf litter, the contents of test five nutrients except Ca were higher in June and July; in branch litter, no notable monthly variation was observed for the test nutrients contents; and in the miscellany, the contents of test nutrients were higher in June and October than in other months.
    Effects of community structure on carbon fixation of urban forests in Shanghai, China.
    2010, 29(03):  439-447. 
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    Based on the RS image digitization, investigations on representative communities, measurement of dominant species physiological variables, and CITYgreen model, this paper evaluated the carbon fixation of urban forests in Shanghai, and studied the effects of community structure on the carbon fixation of the forests. The total carbon storage and the annual carbon sequestration of urban forests in Shanghai were 478472 t and 6256 t, and the average carbon density and av
    erage carbon sequestration rate were 47.80 t·hm-2 and 0.625 t·hm-2·a-1, respectively. The carbon sequestration rate was significantly positively correlated with canopy closure and community density but negatively correlated with average DBH, and the carbon density was significantly positively correlated with canopy closure and average DBH but had no significant correlation with community density. The forests with low density and high DBH had higher carbon density than those with medium or high density. Mixed forests had higher carbon density than pure forests, multilayer forests had higher carbon density and c
    arbon sequestration rate than monolayer forest, and the differences were to some extent affected by average DBH and community density.
    Interspecific association of rare and endangered Pinus bungeana community in Xiaolongshan of Gansu.
    2010, 29(03):  448-453. 
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    Through field investigation, 20 common plant species were selected from 50 plots of Pinus bungeana community in Xiaolongshan to analyze their interspecific associations by using multispecies relevance (variance ratio, VR), χ2test, Ochiai index, Dice index, Jaccard index, AC value, PC value, Pearson’s correlation coefficient, and Spearman’s rank correlation coefficient. The VR analysis showed that the associations between the main components of the community were mainly of positive, demonstrating that the nature of the community was consistent with the characteristics of its living environment, and the community was adapted to its outside environment. The species-pair associations among the 20 common species had different properties, which showed the inner environment of the community and was caused by the heterogeneity of the environment within the community. Considering the ecological characteristics of plants, the community was divided into three ecological species groups. It was found that the high proportion of negative association in the community was caused by the shade shrubs under the trees, and the high independence of the community was caused by the plants from the forest edge. The study of interspecific association could reflec
    t the organic link formed by the ecological characteristics of plant species, an
    d the  results would have certain practical significance for the protect
    ion and restoration of P. bungeana community.
    Effects of temperature on interspecific competition between two bluegreen algae.
    2010, 29(03):  454-459. 
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    A laboratory experiment was conducted to study the interspecific competition between Microcystis aeruginosa and Oscillatoria princeps at 20 ℃, 25 ℃,and 30 ℃. Both in pure culture and in mixed culture, M. aeruginosa grew best at 25 ℃, while O. princeps grew best at 30 ℃. Temperature had obvious effects on the inhibition parameter of interspecific competition between the two algae. The inhibition parameter of O. princeps against M. aeruginosa at 30 ℃ was 1.42 and 1.13 times as that at 20 ℃ and 25 ℃, while the inhibition parameter of M. aeruginosa against O. princeps was the maximum at 25 ℃, being 1.54 and 1.21 times as that at 20 ℃ and 30 ℃, respectively. At 20 ℃, 25 ℃, and 30 ℃, the inhibition parameter of M. aeruginosa against O. princeps was all larger than that of O. princeps against M. aeruginosa, which meant the stronger inhibition effects of M. aeruginosa on O. princeps at the test temperatures. Based on the competition model of LotkaVolterra, M. aeruginosa won the competition at 20 ℃ and 25 ℃, and M. aeruginosa and O. princeps could unstably coexist at 30 ℃.
    Effects of salicylic acid on frost hardiness and electrical impedance spectroscopy parameters of Euonymus japonicus stem.
    2010, 29(03):  460-466. 
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    Currentyear cutting plants of Euonymus japonicus were treated with different concentration salicylic acid (SA) before frost hardening, and their
     frost hardiness during the period of the hardening was assessed by electrical impedance spectroscopy (EIS) and electrolyte leakage (EL), aimed to explore the e
    ffects of SA on the frost hardiness and the EIS parameters of E. japonicus, and to search for the suitable EIS parameters to evaluate the frost hardiness
    of E. japonicus stems with and without artificial freezing. Treating with SA increased the frost hardiness of E. japonicus stems, and the optimal
    concentration of SA was 5.0 mmol·L-1. For the nonfrostexposed stems, the resistances r and r1, extracellular resistance re, intracellular resistance ri, relaxation time τ, and distribution coefficient of relation time ψ had high correlations with the frost hardiness measured by EL (r=0.70-0.87), suggesting that these EIS parameters could be used to measure the frost hardiness of E. japonicus without artificial freezing, and r1 was the best parameter. For the frostexposed stems, the re, τ, and ri were highly correlated with the frost hardiness (r=0.85-0.94), indicating that re, τ, and ri could be the suitable parameters to measure the frost hardiness of E. japonicus after artificial freezing, and re was the best parameter.
    Screening of salttolerance concentration and comparison of salttolerance for chrysanthemum and its related taxa.
    2010, 29(03):  467-472. 
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    Taking Ajania shiwogiku var. kinokuniense, Artemisia japonica, Artemisia vulgaris, and Chrysanthemum indicum var. maruyamanum as test
    plants, a hydroponic experiment was conducted to screen the appropriate concentration for salttolerance, and to compare the salttolerance of test plants. The culture medium Hogland solution was added with 0, 75, 100, 150, 250, and 400 mmol·L-1 of NaCl, and the changes of plant morphological characteristics were observed. The results showed that 100 and 150 mmol·L-1 of NaCl were the appropriate concentrations for testing salttolerance. Obvious injury symptoms appeared at 10 days and 6 days under 100 and 150 mmol·L-1 of NaCl stress, respectively. The salttolerance of the four species was evaluated in terms of root vigor, malondialdehyde and chlorophyll contents, and morphological changes under 120 mmol·L-1 of NaCl. Subordinate function value analysis showed that the salttolerance was in order of Chrysanthemum indicum var. maruyamanum<Ajania shiwogiku var. kinokuniense<Artemisia vulgaris<Artemisia japonica, being consistent with the results evaluated by the index of 50% injured leaf area.
    Effects of different C/N ratios in subsurface Acorus calamus constructed wetland on pollutant removal efficiency.
    2010, 29(03):  473-478. 
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    By using slag and gravel as substrates, and with no plant as the control, three carbon levels (C1, C2, and C3) and three nitrogen levels (N1, N2, and N3) were set to study the removal efficiency of COD, TN, and TP in subsurface Acorus calamus constructed wetland with different C/N ratios. A significantly higher removal efficiency of COD and TN was found in the wetlands with plants, compared with the control. A. calamus increased the removal efficiency of COD and TN by 10.53% and 6.73%, respectively, but had no significant effect in the removal of TP. With the changes of N and P concentrations and C/N ratios in the influent, the removal efficiency of COD, TN and TP in planted wetlands were 67.57%-75.85%, 20.91%-56.82%, and 7.15%-17.78%, respectively. The N and Paccumulations in aboveground part of A. calamus were 4.44-14.79 and 1.11-3.37 g·m-2, occupying 6.91% and 10.67% of the total N and P removal, while those in belowground part of A. calamus were 2.35-5.20 and 0.74-1.41 g·m-2, occupying 2.69% and 6.02% of the total N and P removal, respectively, which implied that it would be helpful to remove the N and P in wetland system through harvesting the aboveground part of plants.
    Flux dynamics of nitrogen and phosphorus in major input rivers of Zhangze Reservoir.
    2010, 29(03):  479-484. 
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    Based on the investigation data of the hydrology and water quality of three input rivers (Nanzhang, Shizi and Jianghe) of Zhangze Reservoir in 2006-2007, this paper studied the main hydrological characteristics and the nitrogen and phosphorus concentrations and their monthly flux dynamics in the rivers. The
    flow speeds of the three rivers were slow, and the water temperature was suitable, with an annual mean value 12.5 ℃-15.1 ℃. The water temperature increased
    rapidly from March to May, and became higher in summer. Accompanying with adequate sunlight, the higher water temperature in summer induced the occurrence of algal bloom. The yearly average concentrations of total nitrogen and phosphorus in the three rivers were 1.75-8.90 mg·L-1 and 0.005-3.760 mg·L-1, respectively, being relatively higher in general. Shizi River contributed 48.3% of the total nitrogen and 77.3% of the total phosphorus to the Zhangze Reservoir. Dissolved inorganic nitrogen was the major form of total nitrogen, which was dominated by nitrate nitrogen (NO3--N), accounting for 60% or more. The
    nitrogen and phosphorus fluxes had no obvious seasonal variation, and their monthly fluctuations were also gentle. Point pollution was the main source of the ni
    trogen and phosphorus in Nanzhang and Shizi rivers, while the nitrogen in Jianghe River was mainly come from nonpoint pollution. To protect the Zhangze Reservoir, it would be critical to control the point source pollution of Nanzhang and Shizi rivers.
    Effects of tillage mode on soil erosion of rainfed cropland in Inner Mongolia.
    2010, 29(03):  485-490. 
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    A field experiment was conducted in the Qingshuihe County of Inner Mongolia to study the soil erosion of rainfed cropland under effects of different
     tillage modes, which would be of significance to the construction of ecological agriculture in the hilly regions of Inner Mongolia. Three treatments, i.e., no
    tillage (NT), notillage with straw mulch (NS), and conventional tillage (CT), were installed, and the test crops were Sesamum indicum and Avena fatua. The annual precipitation, runoff, and soil loss were measured. Compared with CT, treatments NT and NS could markedly reduce soil erosion, e.g., the runoff and soil loss in treatment NS were decreased by 219% and 883%, respectively. Topography had great effects on soil erosion. In general, the soil erosion was increased with increasing gradient. Under the same topographic condition, the soil erosion differed with crop species. The relationships between precipitation and soil loss under the three tillage modes fitted power function, with R2>0.9.
    Effects of different fertilization modes on the budgets and recycling rates of trace elements in agroecosystems.
    2010, 29(03):  491-497. 
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    A group of longterm field trials was conducted at the lower reaches of Liaohe Plain to study the effects of different fertilization modes on the rec
    ycling rates and current season budgets of trace elements in cropland ecosystems. In the process of feedingcomposting, the recycling rates of trace elements v
    aried with the elements. Zn had the lowest recycling rate which still approached to 80%, while Fe, Mn, Zn, and Pb had a recycling rate of>80%, and especially
    that of Fe, Mn and Pb was>90%. The recycling rate increased with the increasing amount of feed stuffs and bedding materials. The trace elements input of farml
    and from manures was far higher than that from chemical fertilizers, and increased with increasing amount of the manures applied; while the trace elements output by crops was the highest in treatment NPK+M, and the lowest in treatment CK. The input was lower than the output, and the budget in different fertilization tr
    eatments followed the order of NPK+M<M<CK<NPK. Therefore, applying chemical fertilizers alone induced the deficit of soil trace elements, while nutrients
    recycling would offset the soil trace elements loss by crops, and minimize the deficit.
    Freshwater residence time in Bohai Sea.
    2010, 29(03):  498-503. 
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    A box model based on freshwater fraction method was used to estimate the freshwater residence time (FRT) in the Bohai Sea. The data used in this study
     include the evaporation, precipitation, river discharge, and salinity in the Bohai Sea and its surrounding region, the Yellow Sea. Sensitivity analysis was con
    ducted to quantitatively assess the effects of freshwater flux on the water exchange between Bohai Sea and Yellow Sea. The results indicated that the interannua
    l variability in the salinity in Bohai Sea was mainly caused by the freshwater supply from Yellow River, the largest river around Bohai Sea. During the periods
    of 1965-1980, 1986-1992, 1994, and 1996, themean FRT in Bohai Sea was 2.6 years, and had a weak upward trend. In the 1960s, the FRT was 3.0 years; but in the 1980s, it increased to 4.6 years, with an increment of about 50%. The FRT decreased with increasing freshwater supply, and in general, was more sensitive to river discharge than to net precipitation flux (the difference between precipitation and evaporation), suggesting that sufficient attention should be paid on the formulation of environmental policy on the regulation of Yellow River water resources.
    Diversity of arbuscular mycorrhizal fungi in Dahurian larch forests in Da Hinggan Ling Mountains.
    2010, 29(03):  504-510. 
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    An investigation was made on the colonization and diversity of arbuscular mycorrhizal fungi (AMF) on the root systems of 26 plant species of 17 families in the Dahurian larch forests in Da Hinggan Ling Mountains. It was found that 23 of the 26 plant species could form arbuscular mycorrhizae (AM). The average
    colonization rate was 21.69%, ranging from 0 to 78.7%. Four plant species formed Paris-type AM, 10 plant species formed Arum-type AM and 9 intermediatetype AM, and 3 species could not form AM. The 50 AMF species isolated from root zone soils belonged to five genera, among which, 24 AMF species belonged to Acaulospora, 19 belonged to Glomus, 5 belonged to Entrophospora, 1 belonged to Archaeospora, and 1 belonged to Gigaspora. Acaulospora and Glomus were the dominant genera, and A. nocolsonii and G. etunicatum were the dominant species. The average AMF spore density was 68.94 per 100 g air-dried soil, and the average species richness was 7.83 species per soil sample. No correlations were observed between the AM colonization rate and the spore density and species richness.
    Quantity of culturable microorganisms and diversity of bacterial physiological groups in transgenic Bt corn rhizosphere.
    2010, 29(03):  511-516. 
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    The transgenic Bt corn Mon810 and its parental nontransgenic corn (the control) were grown in field to study the quantitative changes of culturable
    microorganisms and the diversity of bacterial functional groups in their rhizosphere at different growth stages. There was no significant difference in the colonyforming of cultivable fungi in the rhizosphere of Bt corn and the control across the whole growth period, but significant differences were detected for bact
    eria at silking stage and actinomycetes at seeding stage. The diversity of the same bacterial functional groups in the rhizosphere of Bt corn and the control was similar, but the diversity of different bacterial functional groups varied. The number of aerobic nitrogen-fixing bacteria in the rhizosphere of Bt corn was
    significantly different from that of the control at five growth stages of the whole growth period, while the numbers of ammonifying bacteria, potassiumsolubil
    izing bacteria, and nitrosation bacteria were only significantly different at milking stage, trumpet stage and jointing stage, and tasselling stage, respectively. The numbers of aerobic cellulosedecomposing bacteria and nitrobacteria were significantly different at seedling stage, jointing stage, and trumpet stage, and that of phosphorusdecomposing bacteria was significantly different at milking stage and full- ripe stage. Across the whole growth period except at silking stage and fullripe stage, the population characteristic parameters of the microorganisms in Bt corn rhizosphere were higher than those in nonBt corn rhizosphere.
    Impacts of snow storm in 2008 on soil microbial biomass nitrogen and dissolved nitrogen in Moso bamboo forests in Wuyi Mountains.
    2010, 29(03):  517-522. 
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    In January 2008, an extraordinarily serious snow storm and freezing disaster occurred in southern China, giving obvious impacts on the ecosystem proce
    sses via altering the resources availability and heterogeneity. Taking the Moso bamboo forests with different damaged levels (light, moderate, and heavy) in Wuyi Mountains as test sites, this paper studied the effects of this snow storm on the microbial biomass nitrogen and total dissolved nitrogen in 0-10, 10-25, and
    25-40 cm soil layers. Except the microbial biomass nitrogen content in 25-40 cm soil layer, the contents of microbial biomass nitrogen and nitrate in the three
    soil layers increased with increasing damaged level, and decreased with soil depth. The dissolved organic nitrogen content in 0-10 cm soillayer was significantly higher in heavily damaged bamboo forest than in lightly and moderately damaged bamboo forests. The contents of soil microbial biomass nitrogen, dissolved organic nitrogen, and nitrate in the forests were significantly positively correlated with soil temperature and the ground biomass input caused by the snow storm but negatively correlated with canopy closure, and had no relationship with soil moisture. It was suggested that owing to the changes of biotic and abiotic conditions caused by the snow storm, the soil nitrogen in the forests could be lost from the ecosystem in the forms of nitrate and dissolved organic nitrogen.
    Soil fungi differentially affect the growth of and interaction between invasive weed Ambrosin artemisiifolia and native plants.
    2010, 29(03):  523-528. 
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    A greenhouse pot experiment was conducted to investigate the effects of soil fungi in native plants-dominated soil (NS) and Ambrosin artemisiifolia
    -invaded soil (IS) on the growth of and interaction between invasive weed A. artemisiifolia and native plants. On NS, the biomass of A. artemisiifolia
     from monoculture A. artemisiifolia, mixed culture A. artemisiifolia and native plant S. viridis, and mixed culture A. artemisiifolia and native p
    lant M. sativa in fungicide treatment was 46.7%, 39.1%, and 90.5% higher than that in non-fungicide treatment, respectively, but no significant difference was observed in the biomass of S. viridis and M. sativa between fungicide and nonfungicide treatments. On IS, compared with that in nonfungicide
    treatment, the biomass of A. artemisiifolia from monoculture A. artemisiifolia in fungicide treatment was decreased by 44.3%. Fungicide application also decreased the biomass of the two native plants in mixed cultures, but the effect was not significant. The feedback of soil fungi in NS and IS on plant growth
    and competition was further analyzed. In NS, there was a negative feedback of soil fungi on the growth of A. artemisiifolia, no matter in monoculture or in mixed culture; while in IS, the feedback on the growth of monoculture A. artemisiifolia became positive, and the negative feedback on the growth of mixed culture A. artemisiifolia became less. This study supplied some experimental evidences for the further research on the soil microbial mechanism during A. artemisiifolia invasion.
    Soil enzyme activities in Moso bamboo forests along an altitude gradient.
    2010, 29(03):  529-533. 
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    Soil samples were collected from the Moso bamboo forests with same soil type, slope gradient, slope orientation, and management level at three altitudes (90-120 m, 360-400 m, and 700-780 m) in the mid and south subtropical transitional zone, the southern edge of Moso bamboo distribution, with their physical and chemical properties and enzyme activities determined. In the forests,the activities of soil alkaline phosphatase, invertase, and urease were in the ranges of 0.031-0.042, 0.104-0.146, and 0.017-0.039 mg·g-1·h-1, respectively, with the turning points appeared at altitude 360-400 m. The proteinase activity was in the range of 0.248-0.259 mg·g-1·h-1, and increased slowly with increasing altitude; whereas the catalase activity was in the range of 0.097-0.143 mg·g-1·h-1, and increased significantly with increasing altitude. The enzyme activities at the all three altitudes were less affected by soil physical properties and soil total and available phosphorus and potassium, but had significant positive correlations with soil organic matter, total nitrogen, and available nitrogen. Soil pH had positive effects on the enzyme activities. There existed significant correlations among the test enzyme activities, suggesting that soil enzymatic reactions were both specific and concomitant.
    Effects of velocity increment and duration on critical swimming speed of juvenile darkbarbel catfish.
    2010, 29(03):  534-538. 
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    To investigate the effects of velocity increment and duration on the critical swimming speed (Ucrit) of juvenile darkbarbel catfish (Pelte obag vachelli), the Ucrit was determined with different velocity increment and duration at water temperature 25 ℃. The fish were first tested at the duration of 5, 10, 15, 30, and 60 min at a velocity increment of 15% Ucrit (10 body length), and then, tested at a velocity increment of 5%, 10%, 15%, 20%, and 30% Ucrit with a duration of 20 min. The absolute swimming speed (Ua) of experimental fish decreased from (47.4±0.7) cm·s-1 at 5 min to (39.1±1.5) cm·s-1 at 60 min (P<005), but the Ua in 15 min group was not significantly different from that in 30 min group. The Ua increased from (44.1±0.6) cm·s-1 in 5% Ucrit increment group to (47.0±0.4) cm·s-1 in 10% Ucrit increment group (P<005). There were no significant differences in Ua among the groups of 10%, 15%, and 20% Ucrit, but the Ua in 30% Ucrit increment group was significantly lower than that in other groups (P<0.05). It was suggested that the values of critical swimming speed were profoundly affected by the velocity increment and duration, and the proper duration and velocity increment for darkbarbel catfish were 15-30 min and 10%-20% Ucrit, respectively.
    Grazing and trophic strategy of Ochromonas sp. on Chlorella pyrenoidosa.
    2010, 29(03):  539-542. 
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    When we studied the allelopathic effects of rice straw on the growth of microalga Chlorella pyrenoidosa, a flagellate strain which could strongly graze on C. pyrenoidosa and effectively inhibit the population growth of C. pyrenoidosa was incidentally isolated. The flagellate had two yellow chloroplasts, cell spherical or oval, sometimes metamorphic, and usually freeswimming but occasionally attaching to surfaces with posterior end. The flagellate had two unequal flagella. The longer flagellum was about 1.5 times as the length of the cell, and the shorter one was about half times as the length of the cell. The flagellate showed strong phagocytic capacity on C. pyrenoidosa. Each flagellate could simultaneously swallow 3-4 cells of C. pyrenoidosa, and its body size changed greatly with the quantity of food swallowed. From the morphological features, this flagellate was identified as a species of Ochromonas. The growth curves of the Ochromonas sp. population on three different culture media (SE, rice straw, and wheat grain) showed that wheat grain medium was optimum
     for the population growth. Based on our results, the trophic strategy of Ochromonas sp. was of mixotrophy, but phaghotrophy was the main mode.
    Genetic diversity of wapiti population in eastern Wandashan Mountains of Heilongjiang Province, China based on microsatellite analysis.
    2010, 29(03):  543-548. 
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    The eastern Wandashan Mountains of Heilongjiang Province is one of theareas with high population density of wapiti (Cervus elaphus xanthopygus).
    In this paper, 167 wapiti fecal samples were collected there in two winters to identify their DNA based on 7 microsatellite loci, aimed to study the genetic div
    ersity of the population, and the effects of the population’s dramatic decline in recent years on the population genetic structure. The DNA of the samples was
    assigned to 66 individuals. The average numbers of the alleles and effective alleles were 9.00±2.77 and 3.97±0.99, respectively, the average polymorphism information content was 0.69±0.09, and the average observed and expected heterozygosity values were 0.69±0.08 and 0.74±0.08, respectively. The population was significantly deviated from Hardy-Weinberg Equilibrium, but the inbreeding coefficient was 0.060, not significantly deviated from zero. It was suggested that the genetic diversity of the population was still higher, and the dramatic decline of the population didn’t yet affect the population genetic structure due to time lag.
    Genetic diversity and origins of Chinese native greylag goose breeds based on mtDNA D-loop sequences.
    2010, 29(03):  549-553. 
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    In order to reveal the genetic diversity and systematic evolution of Chinese greylag goose breeds, the 521 bp control region (D-loop) of mitochondrial DNA from 13 greylag goose breeds were sequenced. In the D-loop, the contents of T, C, A, and G nucleotides occupied 23.8%, 29.0%, 32.3%, and 15.0%, and the average haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.19245 and 0.00036, respectively. The nucleotide variance value among the populations was bigger than that within the populations, and no population expansion was observed for the breeds. The shared haplotype analysis and systemic evolution analysis revealed that the 13 breeds had two maternal origins, among which, Yili breed originated from greylag goose (Anser anser), and the other 12 breeds originated from swan goose (A. cygnoides).
    Effects of predatory stress imposed by Harmonia axyridis on the development and fecundity of Drosophila melanogaster.
    2010, 29(03):  554-559. 
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    Model insect Drosophila melanogaster was exposed to its predatory natural enemy Harmonia axyridis to study the responses of the insect in its
    development and fecundity. When the male and female D. melanogaster adults were separately exposed to H. axyridis, the lifespan of the males was significantly extended, while that of the females was less affected; when the male and female D. melanogaster adults were exposed together to H. axyridis, the development duration of D. melanogaster larvae and the lifespan of the males were prolonged. There were some changes in the development duration of successive stages when the 1st instar D. melanogaster larvae were exposed directly or indirectly to the predator, being significantly longer under indirect than under direct exposure. To our knowledge, this is the first report to observe the changes in the developmental duration and fecundity of female and/or male D. melanogaster exposed to H. axyridis.
    Influence of wind power field on birds in Yancheng National Rare Waterfowls Nature Reserve of Jiangsu.
    2010, 29(03):  560-565. 
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    Based on the longterm monitored data on birds and shortterm field observation, in combining with the environmental characteristics of Yancheng National Rare Waterfowls Nature Reserve, this paper analyzed the influence of wind power field on the birds in the Reserve. Owing to the occupation of avian habitat, the establishment of wind power field had indirect effects on the inhabiting behaviors and foraging of birds, but the impact was relatively small. The slow fan speed and the higher altitude at which the birds fly led to a lower collision risk between fan and birds. In order to protect the safety of birds and their normal life, some countermeasures such as rational distribution of wind power fields, establishment of bird observation stations, and coordination of developing and constructing regional beach and adjacent areas were proposed.
    Distribution pattern and related habitat factors of invasive plant Eupatorium adenophorum along the roadsides in central Yunnan Province, China.
    2010, 29(03):  566-571. 
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    In order to understand the distribution pattern and related habitat factors of invasive herb Eupatorium adenophorum along the roadsides in central
     Yunnan, an investigation was made in 12 transects of four land cover types (shrubland, Pinus yunnanensis forest, Eucalyptus smithii plantation, and secondary semi-humid evergreen broadleaved forest) in Chuxiong, Yunnan Province. The investigation showed that the distance from roads had significant effects on the cluster number, cluster area, height, cover, and seed number of E. adenophorum. These five parameters reached their peaks at 5 m from the roadsides, and then declined. Land cover type also had significant effects on the cluster number, cluster area, cover, and seed number of E. adenophorum, e.g., secondary semi-humid evergreen broad-leaved forest could better inhibit the invasion and diffusion of E. adenophorum. The principal component analysis demonstrated that the entire ecological message about the habitat factors could be symbolized by three principal components, i.e., “light and biotic factors” (component 1), “water and heat” (component 2), and “topography” (component 3). The coverage of E. adenophorum was significantly negatively correlated with the coverage of trees and their species richness, and positively correlated with light intensity, suggesting that the increasing coverage of native trees under good protection could better inhibit the invasion and diffusion of E. adenophorum.
    Spatial pattern and cluster analysis of seed plant floristic elements in Yunnan,China.
    2010, 29(03):  572-577. 
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    Based on allaround floristic information, and by using geographical information system and cluster analysis, this paper studied the spatial pattern of seed plant floristic elements in Yunnan Province of China. It was indicated that the proportions of cosmopolitan and temperate elements increased with increasing latitude, while those of tropical elements had a decreasing trend. Cluster analysis showed that the study area could be divided into two parts, with the latitude about 24.5°N as a division line. To the south of the line, the dominant flora was tropical elements, and the vegetation was dominated by tropical mountain rainforest, seasonal rain forest, monsoon rain forest, and monsoon evergreen broadleaved forest. To the north of the line, there were much greater proportions of temperate elements, and the vegetation was dominated by the evergreen, hard and broadleaved forest, temperate coniferous forest, coniferous and broadleaved mixed forest, alpine meadow, and evergreen broadleaved forest (moist or semimoist, showing strong floristic transition). This division line was different from “Tanaka Line”, possibly due to different study aims and contents.
    Responses of vegetation phenology in Northeast China to climate change.
    2010, 29(03):  578-585. 
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    l data, and by using GIS spatial analysis and statistical methods, the responses of vegetation phenology in Northeast China to climate change were analyzed. From 1982 to 2003, the annual mean temperature in Northeast China had an increasing trend, while the annual mean precipitation was in reverse. The elevated temperature in spring advanced the beginning dates of the growth seasons of the vegetations in coniferous forest, coniferous and broadleaved mixed forest, broadleaved forest, meadow, and swamp (P<0.05), while the precipitation in spring only postponed the beginning date of the growth season of the vegetation in coniferous forest (P<0.05). Temperature had less effects on the ending dates of the vegetations growth seasons except that the declined temperature in autumn advanced the ending dates of the growth seasons of grasses (P<0.05). Compared with temperature, precipitation had more effects on the ending dates of the vegetations growth seasons. Due to the decreased precipitation in autumn, the ending dates of the growth seasons of the vegetations in coniferous and broadleavedmixed forest, grassland, and cropland were advanced (P<0.05), while those of the growth seasons of grass-forb communities advanced  (P<0.05). The ending dates of crop growth seasons were advanced by the declined precipitation in summer and September (P<0.05). The warmer temperature in spring prolonged the growth seasons of the vegetations in broadleaved forest and swamp (P<0.05). The declined precipitation in spring shortened the growth seasons of shrubs (P<0.05), while the increased precipitation in summer prolonged the growth seasons of grassforb communities and crops (P<0.05).
    Ecological suitability of olive in Sichuan Province: Fuzzy comprehensive evaluation based on GIS.
    2010, 29(03):  586-591. 
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    In this paper, the ecological suitability of olive in Sichuan Province was evaluated, based on GIS-fuzzy comprehensive evaluation method. A comprehensive consideration was taken on the climatic, soil, and topographic factors related to olive growth. The spatial data of the factors were organized and computed
     with GIS method; the weights of the factors were derived by using AHP method; and the proper membership function and fuzzy arithmetic operators were selected to conduct the comprehensive ecological suitability evaluation. There was a general consistency between the results yielded by GIS-fuzzy comprehensive evaluation method and the actual distribution of olive plantations. Compared with traditional evaluation methods, the GIS-fuzzy comprehensive evaluation method had the advantages of short-term, more fine and detailed, and more suitable for small spatial scale areas.
    Estimation of China ecological footprint production coefficient based on net primary productivity.
    2010, 29(03):  592-597. 
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    Ecological footprint (EF), as a kind of biophysical indicator in measuring the humanity’s use extent of natural resources, is considered as one of the
     most successful methods to assess the sustainable development, and has gained much attention on national and international level since it was first used by Wackernagel and Rees in 1996. Production coefficient is an important parameter in EF model, and directly affects the reliability of the results. In order to make c
    omparisons among different regions in China, production coefficient was introduced to relate the regional primary productivities of different land types (i.e.,
    cropland, pasture, forest land, and fishing ground) to the integrated average primary biomass productivity of the corresponding land type. Aimed to analyze the
    ecological footprint of different areas in regional scale, this paper calculated the production coefficient on national level and provincial level based on the
    net primary productivity (NPP) from MODIS data with 1-km resolution in 2001. On national level, the production coefficient of cropland, forest land, pasture, a
    nd fishing ground was 1.74, 0.86, 0.51, and 0.74, respectively, which meant that the cropland productivity in China was bigger than the global average one. On provincial level, the production coefficient differed with provinces, because the NPP of different land use types in different regions was varied.
    Soil pollution processes, their affecting factors, and phytoremediation of chromium slag heads: A review.
    2010, 29(03):  598-604. 
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    Soil pollution of chromium slag heads has become an important environmental issue, and more and more attention has paid to the topic. In order to have
     a more detailed understanding of this pollution, two basic soil pollution processes, i.e., horizontal and vertical migration of chromium, as well the related a
    ffecting factors, including soil organic matter, pH, Eh, water capacity, soil type, composition of soil inorganic colloids, and flowing direction of groundwater
    , were analyzed. Meanwhile, the research progress on the identification and screening-out of chromium hyperaccumulators, accumulation mechanisms of chromium hyperaccumulators, and phytoremediation of contaminated soils around chromium slag heads and its mechanisms were summarized. It was considered that the use of chromium hyperaccumulators to remediate the contaminated sites of chromium slag heads would have a wide prospect, though the phytoremediation of chromiumcontaminated soils is still at its initial stage.
    Habitat characteristics of Presbytis leucocephalus in Guangxi Zhuang Autonomous Region.
    2010, 29(03):  605-610. 
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    Presbytis leucocephalus is an endemic species only found in China. Its habitats are quite narrow and disperse. In Guangxi Zhuang Autonomous Region, the “karst rocky mountain” landscape composed of many kinds of limestone and special limestone monsoon forest or secondary vegetation is a typical tropical karst geomorphologic assemblage of clusterpeak depression and cluster valley, and different from the “earthy mountain” landscape around. Environmental heterogeneity and uncontinuity make the “karst rocky mountain” landscape become isolated and homogeneous patches with clear boundaries, being able to provide critical living space for P. leucocephalus in cave and groupliving. P. leucocephalus is a species adaptable to tropical climate, good at climbing, phytophagy, and caveliving. It chooses low karst clusterpeak depression and cluster valley as its suitable habitat, which is the result of its ecological adaptation in its longterm evolution process. At present, P. leucocephalus only distributes in several isolated areas. In recent decades, due to the intense disturbance of human activities, the habitat size of P. leucocephalus reduces rapidly, and the habitat distribution becomes much more disperse. Only by properly handling the manland relationship and the manwild animal relationship, forbidding the overexploitation of “karst rock mountain” areas, and maintaining the integrity and landscape heterogeneity of this natural complex, can P. leucocephalus have the survival possibility and development opportunity.