Abstract: Corispermum candelabrum, a typical psammophyte, is widely distributed around semiactive sand dunes of Horqin sandy land. Studies on roots in response to sand microhabitats would help us to deeply understand plant’s mechanisms of adaptation to environmental disturbance or stress in sand dunes. In this study, the excavation method was applied to harvest roots in different soil layers and analyze root morphological variation in different microhabitats along sand dunes (transitional zone, bottom of windward slope, middle of windward slope, the top of sand dune, leeward slope). The results showed that most roots occurred in the layer of 0-10 cm and root diameter was usually less than 1 mm. Along transitional zone to the top of sand dune, aboveground biomass, belowground biomass, root length density, root surface area density and tissue density decreased, while increased at leeward slope. Other indexes such as specific root length and specific root surface area displayed an opposite trend. Roots showed a significant correlation with soil moisture in different microhabitats. We concluded that plants growing in transitional zone with adequate water and nutrients usually take advantages in larger root systems. On the other hand, when growing in the top of sand dune with strong wind erosion and sand burial and water deficit, they usually showed strong efficiency of resource absorption. That means this species has developed reasonable roots allocation for sandy microhabitats.