Optmization for the reaction system of birch ISSR-RCR

Abstract

Abstract: In this paper,the influence of DNA concentration, Tag DNA polymerase and annealing temperature on the result of ISSR-PCR amplification was analyzed using the materials of birch DNA. Seventeen primers, which produced clear and rich polymorphism bands, were screened. The stable and reproducible optimum reaction system of ISSR PCR and PCR amplification parameter were established for birch. The result provided a standardizing program for the analysis of interspecies genetic diversity of Birch.

Key words: Wheat, Drought, Waterlogging, Nitrogen supply, Photosynthesis, Starch accumulation

CLC Number:

S792.153

JIANG Jing, YANG Chuanping, LIU Guifeng, WU Jinhua, FAN Zhiyong. Optmization for the reaction system of birch ISSR-RCR[J]. cje, 2003, (3): 91-93.

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Optmization for the reaction system of birch ISSR-RCR

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