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落叶松和水曲柳带状混交对土壤微生物群落功能多样性的影响

邓娇娇,周永斌,杨立新,张淞著,李慧,魏亚伟,邓继峰,秦胜金,朱文旭*   

  1. (沈阳农业大学林学院, 沈阳110866)
  • 出版日期:2016-10-10 发布日期:2016-10-10

Effects of mixed Fraxinus mandshurica and Larix olgensis plantation on the function diversity of soil microbial community.

DENG Jiao-jiao, ZHOU Yong-bin, YANG Li-xin, ZHANG Song-zhu, LI Hui, WEI Ya-wei, DENG Ji-feng, QIN Sheng-jin, ZHU Wen-xu*#br#   

  1. (College of Forestry, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2016-10-10 Published:2016-10-10

摘要: 为了探讨造林模式对土壤理化性质和微生物群落功能多样性的影响,以辽宁省新宾陡岭水曲柳(Fraxinus mandshurica)、长白落叶松(Larix olgensis)人工纯林及二者混交林土壤为研究对象,对不同林型土壤pH值、养分含量和土壤微生物活性进行研究,对3种人工林土壤微生物代谢功能多样性进行分析。结果表明:混交林土壤全氮、速效氮、全磷和速效磷分别比落叶松纯林增加28.1%、35.6%、9.5%和12.9%;不同人工林土壤微生物群落代谢活性差异显著,总碳源的利用能力(AWCD)的顺序为水曲柳林>混交林>落叶松林,落叶松纯林土壤微生物代谢最慢,活性最弱;3种不同人工林土壤微生物群落对六大碳源的利用率存在差异,混交林在L-精氨酸、L-天门冬酰胺、丙酮酸甲酯、腐胺、N-乙酰-D-葡萄糖胺、D-半-乳糖醛酸、D-葡萄糖胺酸和4-羟基苯甲酸等8种碳源的利用能力显著高于落叶松纯林;主成分分析显示,糖类和氨基酸类碳源是影响微生物活性的重要碳源;3种不同人工林土壤微生物优势度指数无显著差异,物种丰富度指数依次为水曲柳林>混交林>落叶松林;落叶松和水曲柳带状混交后可以提高土壤肥力及土壤微生物活性。

关键词: 封丘县, 农业景观, 景观异质性, 两栖类, 黄河下游地区

Abstract: In order to evaluate the effect of afforestation mode on soil physical and chemical properties and the function diversity of microbial community, we studied soil pH, nutrient content and metabolism function diversity of soil microorganisms in three plantation forests, including pure coniferous forest (Larix olgensis), pure broadleaf ash forest (Fraxinus mandshurica), and their mixed forest (F. mandshurica + L. olgensis) in Douling, Xinbin of Liaoning Province. The results showed that, compared to the coniferous forest, the concentrations of total N, available N, total P and available P in the mixed forest increased by 37.1%, 35.6%, 9.5% and 12.9%, respectively. Total carbon source utilization capacity (AWCD) by soil microbial communities was in the order of F. mandshurica forest > mixed forest > L. olgensis forest, indicating that soil microbial metabolic activity in L. olgensis forest was the lowest. There were differences in microbial utilization rate of six major carbon sources among the three forests. The mixed forest had significantly higher microbial utilization rates in L-arginine, Lasparagine, pyruvic acid methyl ester, putrescine, N-acetylD-glucosamine, D-galacturonic acid, D-glucosaminic acid, and 4-hydroxybenzoic acid (eight kinds of carbon source) than the coniferous forest. Principal component analysis showed that carbohydrate and amino acids were important factors affecting soil metabolic activity. There was no significant difference in Simpson’s diversity index of soil microbes among the three forests. The Shannon’s index was in order of F. mandshurica forest > mixed forest > L. olgensis forest. Our results demonstrated that planting F. mandshurica and L. olgensis trees together can improve soil fertility and promote soil microbial activity.

Key words: agricultural landscape, landscape heterogeneity, Fengqiu Country, the lower reaches of the Yellow River., amphibians