基于RAPD标记的皖南山核桃野生居群遗传多样性分析

生态学杂志

基于RAPD标记的皖南山核桃野生居群遗传多样性分析

郭传友^**,王贝贝,吕岩桢,张兴旺

（淮北师范大学生命科学学院, 资源植物生物学安徽省重点实验室, 安徽淮北 235000）

出版日期:2015-05-10 发布日期:2015-05-10

RAPD analysis on genetic diversity of wild populations of Carya cathayensis in southern mountainous area of Anhui Province.

GUO Chuan-you^**, WANG Bei-bei, LU Yan-zhen, ZHANG Xing-wang

(School of Life Sciences, Anhui Key Laboratory of Plant Resources and Biology, Huaibei Normal University, Huaibei 235000, Anhui, China)

Online:2015-05-10 Published:2015-05-10

摘要/Abstract

摘要：

应用随机扩增多态性DNA(RAPD)分子标记方法分析了皖南山区4个山核桃（Carya cathayensis）野生居群的遗传多样性及其分化程度。由POPGENE软件分析表明，15条随机引物共检测出139个扩增条带，其中多态性条带87个，多态性位点百分比(PPB)为62.59%，各居群平均多态性位点百分比(PPB)为43.35%。物种水平Shannon信息多样性指数(I)为0.208，Nei基因多样性指数（H）为0.124，多样性水平较低。由于受到地理隔离的影响，居群间的基因流较低（N_m=0.700），遗传分化程度相对较高(G_st=0.417)。

关键词: 连作, 库容量, 源活性, 马铃薯, 库活性, 库源关系

Abstract:

Genetic diversity and genetic differentiation were examined in four wild populations of Carya cathayensis in southern mountainous area of Anhui Province using random amplified polymorphic DNA (RAPD) markers. As analyzed by POPGENE software, a total of 139 discernible loci were obtained from all populations using 15 random primers, 62.59% of which (87 fragments) were polymorphic loci, with an average percentage of polymorphic loci as 43.35% for all populations. At species level, Nei’s genetic diversity (H=0.124) and Shannon’s information index (I=0.208) indicated that C. cathayensis had a relatively low genetic diversity. The higher value of differentiation (G_st=0.417) and the lower value of gene flow (N_m=0.700) showed that the geographic isolation has an obvious influence among populations.

Key words: continuous cropping, sink-source relationship, sink size, source activity, potato, sink activity

郭传友**,王贝贝,吕岩桢,张兴旺. 基于RAPD标记的皖南山核桃野生居群遗传多样性分析[J]. 生态学杂志.

GUO Chuan-you**, WANG Bei-bei, LU Yan-zhen, ZHANG Xing-wang. RAPD analysis on genetic diversity of wild populations of Carya cathayensis in southern mountainous area of Anhui Province.[J]. cje.

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