六种常见抗生素对小白菜种子萌发及生理特性的影响

生态学杂志

六种常见抗生素对小白菜种子萌发及生理特性的影响

肖明月¹,安婧^2**,纪占华^2,3,崔爽¹,李萍¹

（¹辽宁石油化工大学，辽宁抚顺 113001； ^2中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室，沈阳 110016； ^3中国科学院大学，北京 100049）

出版日期:2014-10-10 发布日期:2014-10-10

Toxic effects of six typical antibiotics on seed germination and physiological characteristics of Chinese cabbage.

XIAO Ming-yue¹, AN Jing^2**, JI Zhan-hua^2,3, CUI Shuang¹, LI Ping^1

(¹Liaoning Shihua University, Fushun 113001, Liaoning, China; ²Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ³ University of Chinese Academy of Sciences, Beijing 100049, China)
.

Online:2014-10-10 Published:2014-10-10

摘要/Abstract

摘要：

以小白菜为受试生物，研究了6种常用抗生素（四环素、土霉素、金霉素、强力霉素、氯霉素和磺胺二甲嘧啶）对小白菜种子萌发、根（芽）伸长、可溶性蛋白及抗氧化酶系统的毒理效应。结果表明，0.5～5 mg·L^-1抗生素胁迫下，小白菜种子的发芽率无显著变化，随着抗生素暴露浓度的增加，小白菜种子的发芽率受到了显著抑制；抗生素暴露浓度与小白菜根（芽）伸长抑制率之间具有良好的剂量效应关系。由抗生素对小白菜根长的半抑制效应浓度（EC₅₀）可知，6种抗生素对小白菜种子萌发的毒性大小依次为：磺胺二甲嘧啶>氯霉素>金霉素>四环素>强力霉素>土霉素。实验浓度范围内的抗生素对小白菜幼苗体内可溶性蛋白含量具有显著的诱导效应，且各处理组间差异显著。此外，抗生素的胁迫破坏了小白菜幼苗体内的抗氧化防御系统的平衡。

关键词: 增温, 形态, 抗氧化, 小嵩草, 藏嵩草

Abstract: Chinese cabbage (Brassica chinensis L.) was selected to investigate the toxic effects of six antibiotics (tetracycline, oxytetracycline, chlorotetracycline, doxycycline, chloromycetin and sulfadimidine) on the seed germination, root (shoot) elongation, soluble proteins and antioxidant enzymes of the Chinese cabbage. The results showed that 0.5-5 mg·L^-1 antibiotics did not inhibit the germination rate, while the seed germination was significantly inhibited with the increasing of antibiotics concentration. There was a good doseeffect relationship between the antibiotic concentration and the inhibition rate of root (shoot) elongation. According to the EC₅₀ values of root inhibition of the Chinese cabbage stressed by the antibiotics, the toxicity of the antibiotics was in order of sulfadimidine > chloramphenicol > chlortetracycline > tetracycline > doxycycline > xytetracycline. The contents of soluble proteins of Chinese cabbage induced by the antibiotics were significantly different among the groups. Moreover, the balance of the antioxidant enzyme system of Chinese cabbage seedlings was destroyed by the tested antibiotics.

Key words: oxidation resistance, Kobresia pygmaea, K. tibetica, warming, morphology

肖明月1,安婧2**,纪占华2,3,崔爽1,李萍1. 六种常见抗生素对小白菜种子萌发及生理特性的影响[J]. 生态学杂志.

XIAO Ming-yue1, AN Jing2**, JI Zhan-hua2,3, CUI Shuang1, LI Ping1. Toxic effects of six typical antibiotics on seed germination and physiological characteristics of Chinese cabbage.[J]. cje.

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