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NaCl胁迫对姜黄组培苗生理特性的影响

杨晨,刘建福**,王明元,王奇志,唐源江,钟书淳   

  1. (华侨大学生物工程与技术系, 福建厦门 361021)
  • 出版日期:2014-02-10 发布日期:2014-02-10

Effects of NaCl stress on physiological characteristics of in vitro Curcuma aromatica plantlets.

YANG Chen, LIU Jian-fu**, WANG Ming-yuan, WANG Qi-zhi, TANG Yuan-jiang, ZHONG Shu-chun   

  1. (Department of Biological Engineering and Technology, Huaqiao University, Xiamen 361021, Fujian, China)
  • Online:2014-02-10 Published:2014-02-10

摘要:

以姜黄组培苗为试验材料,在继代培养基中分别添加0、25、50、75、100、125、150 mmol·L-1 NaCl进行盐胁迫处理,研究NaCl胁迫下姜黄幼苗生长和生理特性的变化。结果表明:NaCl胁迫下,姜黄组培苗的脯氨酸和丙二醛含量逐渐上升,而叶绿素和类胡萝卜素含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性逐渐下降。25 mmol·L-1 NaCl时姜黄幼苗的基径、株高和鲜重与对照差异不显著,>50 mmol·L-1 NaCl时则显著下降;表明姜黄组培苗的生长可以耐受25~50 mmol·L-1 NaCl胁迫。随着NaCl浓度增加,姜黄幼苗的可溶性糖和蛋白质含量、过氧化物酶(POD)活性先升高后降低,75 mmol·L-1 NaCl时均达到最大值。50~100 mmol·L-1 NaCl胁迫下姜黄叶片的苯丙氨酸解氨酶(PAL)活性和姜黄素含量得到显著提高,表明50~100 mmol·L-1 NaCl胁迫对姜黄素的合成有促进作用。可见,25~50 mmol·L-1 NaCl能够促进姜黄植株的生长,而75 mmol·L-1 NaCl显著提高次生代谢产物姜黄素的含量。
 

关键词: 产量, 微喷带长宽, 水分特性, 叶绿素荧光特性, 水分利用效率, 小麦

Abstract: A tissue culture experiment was conducted to investigate the changes of the growth, physiological characteristics and curcumin concentration of in vitro Curcuma aromatica plantlets under salt stress (0, 25, 50, 75, 100, 125 or 150 mmol·L-1 of NaCl). Results showed that the concentrations of proline and malondialdedyde (MDA) of in vitro C. aromatica plantlets increased, while the concentrations of chlorophyll and carotenoid and the activities of superoxide dismutase (SOD) and catalase (CAT) decreased significantly under salt stress. No significant differences in the basal diameter, height and fresh weight were observed between the 25 mmol·L-1 NaCl and control treatments, while those significantly decreased under over 50 mmol·L-1 NaCl, suggesting that the plantlets of C. aromatica could tolerate 25-50 mmol·L-1 NaCl stress. With the stress increasing, the soluble sugar concentration and peroxidase (POD) activity in C. aromatica increased in the early phase of stress and decreased subsequently, and the maximum was present under 75 mmol·L-1 NaCl stress. The phenylalanine ammonia lyase (PAL) activity and curcumin concentration in leaves significantly improved under 50-100 mmol·L-1 NaCl treatment, indicating that this salt stress promoted the formation of curcumin. Therefore, the range of 25-50 mmol·L-1 NaCl benefits the growth of C. aromatica plantlets, while the 75 mmol·L-1 NaCl increases the curcumin concentration significantly.

Key words: wheat, water characteristics, grain yield, water use efficiency., micro-sprinkling hose length and width, chlorophyll fluorescence-characteristics