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中华小长臂虾对温度的耐受性及温度对其呼吸代谢的影响

姜宏波1,包杰1,丛岩懿1,申旭东1,潘帝希1,于业辉1,李晓东1,2*
  

  1. 1沈阳农业大学畜牧兽医学院, 沈阳 110866; 2盘锦光合蟹业有限公司, 辽宁盘锦 124200)
  • 出版日期:2016-08-10 发布日期:2016-08-10

Temperature tolerance and the effects of temperature on respiratory metabolism of Palaemonetes sinensis.

JIANG Hong-bo1, BAO Jie1, CONG Yan-yi1, SHEN Xu-dong1, PAN Di-xi1, YU Ye-hui1, LI Xiao-dong1,2*#br#   

  1. (1 College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China; 2 Panjin Guanghe Crab Ltd., Co., Panjin 124200, Liaoning, China).
  • Online:2016-08-10 Published:2016-08-10

摘要:

为了研究中华小长臂虾(Palaemonetes sinensis)对温度的耐受性及温度对其呼吸代谢的影响,利用逐渐升降温的方式获得了中华小长臂虾的温度耐受范围,在此基础上以静水密闭式方法研究了温度对中华小长臂虾耗氧率(OCR)、排氨率(AER)和窒息点(AP)的影响。结果表明:中华小长臂虾具有较强的温度耐受能力,耐受范围为0~37 ℃。低温组在5 ℃时活力开始减弱,下降到0 ℃时中华小长臂虾失去运动能力,0 ℃保持6 h后恢复室温则全部死亡;高温组在31 ℃时活力开始下降,34 ℃时出现异常反应,37 ℃开始死亡并在39 ℃全部死亡;温度对中华小长臂虾的OCR、AER和AP均具有极显著影响(P<0.01);在温度为10~30 ℃范围内,随着温度的升高,中华小长臂虾的OCR和AER逐渐升高,回归分析表明,OCR与温度(T)的回归方程为OCR=-0.077+0.018T (R2=0.955);AER与T的回归方程为AER=-0.008+0.002T(R2=0.915)。AP则是随着温度的升高,出现了先升高后下降的变化趋势,在本实验的温度条件下,窒息点的范围为0.19~0.31 mg·L-1;中华小长臂虾氧氮比(O/N)在各个温度处理组的变化范围为7.61~13.24,说明中华小长臂虾在实验温度条件下主要以蛋白质为供能物质,脂肪供能所占的比率较小。
 

关键词: 小兴安岭, 鹿科动物, 适宜生境, 森林经营, 最大熵模型

Abstract: In order to understand temperature tolerance and respiratory metabolism of Palaemonetes sinensis, effects of temperature on survival and oxygen consumption rate (OCR), ammonia excretion rate (AER), asphyxiation point (AP) of P. sinensis were studied in laboratory. The results showed that P. sinensis possessed strong temperature tolerance. Tolerance range of temperature was 0-37 ℃. In low temperature groups, P. sinensis began to decrease activity when temperature fell to 5 ℃ and lost motor ability at 0 ℃. All shrimps were dead after keeping 6 h at 0 ℃ then transferring to room temperature. In high temperature groups, P. sinensis began to decrease activity when temperature increased to 31 ℃ and an abnormal reaction appeared at 34 ℃. Some of shrimps died at 37 ℃ and all shrimps were dead when temperature increased to 39 ℃. Temperature had extremely significant effects on OCR, AER and AP (P <0.01). The OCR and AER of P. sinensis increased with the increase of temperature at 10-30 ℃. Regression analysis showed that OCR and AER had a significant linear correlation with temperature. The equations were OCR=-0.077+0.018T (R2=0.955) and AER=-0.008+0.002T (R2=0.915), respectively. With increasing temperature, AP of P. sinensis increased at first and decreased subsequently. The range of AP was 0.19-0.31 mg·L-1 at 10-30 ℃. The range of O/N ratio at different temperatures was 7.61-13.24, indicating that P. sinensis took protein as the major energy source and fat as secondary.

Key words: MaxEnt model, Lesser Xing’an Mountains., forest management, Cervids, suitable habitat