一株分离自海水的产生物表面活性剂菌株的鉴定及其发酵优化

生态学杂志

一株分离自海水的产生物表面活性剂菌株的鉴定及其发酵优化

杜瑾,郝建安,张晓青,王建艳,张爱君,王静^*

（国家海洋局天津海水淡化与综合利用研究所，天津 300192）

出版日期:2016-05-10 发布日期:2016-05-10

Identification and fermentation optimization of a biosurfactant-producing strain isolated from seawater.

DU Jin, HAO Jian-an, ZHANG Xiao-qing, WANG Jian-yan, ZHANG Ai-jun, WANG Jing^*

(Institute of Seawater Desalination and Multipurpose Utilization, State Oceanic Administration, Tianjin 300192, China).

Online:2016-05-10 Published:2016-05-10

摘要/Abstract

摘要：

为研究分离自海水的芽孢杆菌dhs330产生物表面活性剂的培养条件和产物特性，采用16S rDNA基因序列分析鉴定菌种，对发酵培养基的碳源、氮源、pH值和培养温度进行优化，采用飞行时间质谱进行产物鉴定及抑菌圈法考察产物抑菌活性。结果显示，该菌株与Bacillus mojavensis菌株16S rDNA的序列相似性为99%。菌株发酵液表面张力可由70 mN·m^-1降低至27 mN·m^-1。发酵培养基的最适碳源、有机氮源和无机氮源分别为甘油、酵母膏和尿素；pH值为6.5～7.0、温度为30～35 ℃条件下，菌体生长和生物表面活性剂合成最为有利。发酵产物为脂肽糖脂混合型生物表面活性剂，对海洋污损微生物Bacillus pumilus dhs04有显著的抑菌活性。菌株dhs-330是能合成脂肽糖脂混合生物表面活性剂、具有海洋污损微生物防除潜力的优选菌株。

关键词: 重庆, 冬小麦, APSIM模型, 调参, 验证

Abstract: This experiment studied a biosurfactantproducing bacterial strain isolated from seawater, designated as dhs-330. Phylogenetic analysis of the homoeologous 16S rDNA gene sequences, the optimization of culture conditions of carbon source, nitrogen source, pH and temperature, MALDITOF Mass Spectrometry identification of the products, and antimicrobial activity assay were carried out. The 16S rDNA sequence of Bacillus sp. dhs330 shared a 99% sequence similarity with Bacillus mojavensis. Bacillus sp. dhs-330 decreased the surface tension of culture medium from 70 mN·m^-1 to 27 mN·m^-1. It could grow well with high production of biosurfactants under the following conditions: glycerol as carbon source, yeast extract as organic nitrogen source, urea as inorganic nitrogen source, initial pH 6.5-7.0 and 30-35 ℃. Its principal products were blendbiosurfactants of lipopeptide and glycolipid, which showed antimicrobial activity against marine fouling bacteria Bacillus pumilus dhs04. Strain dhs330 presented a potential application in blend-biosurfactant production and anti-fouling technology.

Key words: Chongqing City, winter wheat, APSIM model, calibration, validation.

杜瑾,郝建安,张晓青,王建艳,张爱君,王静. 一株分离自海水的产生物表面活性剂菌株的鉴定及其发酵优化[J]. 生态学杂志.

DU Jin, HAO Jian-an, ZHANG Xiao-qing, WANG Jian-yan, ZHANG Ai-jun, WANG Jing*. Identification and fermentation optimization of a biosurfactant-producing strain isolated from seawater.[J]. cje.

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