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生态学杂志 ›› 2010, Vol. 29 ›› Issue (09): 1736-1742.

• 研究报告 • 上一篇    下一篇

转基因大豆对根际固氮细菌群落多样性的影响

赖欣,张永生,赵帅,李刚,杨殿林   

  1. 农业部环境保护科研监测所,农业部转基因生物生态环境安全监督检验测试中心, 天津 300191
  • 出版日期:2010-09-08 发布日期:2010-09-08

Effects of transgenic soybean on the diversity of nitrogen-fixing bacteria in rhizosphere soil.

LAI Xin, ZHANG Yong-sheng, ZHAO Shuai, LI Gang, YANG Dian-lin   

  1. Agro-Environmental Protection Institution|Eco-safety Supervision, Inspection &|Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Tianjin 300191, China
  • Online:2010-09-08 Published:2010-09-08

摘要: 为了研究种植转基因大豆在土壤生态系统中对固氮细菌的影响,应用PCR-DGGE技术分析种植不同大豆处理下土壤细菌固氮酶nifH基因的分子多样性。结果表明:低GC含量的厚壁菌门出现在DGGE图谱的上部,而且受生育时期影响比较明显;绿菌门相对保守,不受生育时期和种植品种的影响;在测序得到的46个结果中,共计28个属于变形菌门,占60.8%,广泛分布于DGGE胶的各个部位,大部分较稳定,不受种植品种或者采样时间的影响。相对于不同生育时期对根际土壤固氮细菌的影响,种植转基因大豆、亲本非转基因大豆和普通大豆之间的差异并不明显,表明种植转基因大豆对土壤固氮细菌没有明显的影响。

关键词: 水曲柳, 磷胁迫, 叶绿素合成, 光合作用, 生物量分配

Abstract: In order to understand the effects of planting transgenic soybean on the nitrogen-fixing bacteria in rhizosphere soil, PCR-DGGE was used to study the molecular diversity of nifH genes in the plots planted with transgenic soybean, parent non-transgenic soybean, and local soybean. The sequences showed that low GC Firmicutes were on the top of the DGGE gel, and were significantly affected by sampling date. Chlorobi were not affected by either sampling date or soybean variety. Among the 46 sequences, 28 sequences were of Proteobacteria, occupying 60.8% of the total and showing in all area of the DGGE gel, and most of them were stable, not affected by sampling date or soybean variety. No significant differences were observed in the diversity of nitrogen-fixing bacteria in rhizosphere soils among the treatments of planting transgenic soybean, parent non-transgenic soybean, and local soybean at different sampling dates, suggesting that planting transgenic soybean had less effects on the diversity of nitrogen-fixing bacteria in rhizosphere soil.

Key words: Fraxinus mandchurica, Phosphorus stress, Chlorophyll biosynthesis, Photosynthesis, Biomass partitioning