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生态学杂志 ›› 2005, Vol. ›› Issue (2): 159-162.

• 研究报告 • 上一篇    下一篇

铅对蟾蜍肝、肾过氧化物酶和酯酶同工酶的影响

贾秀英, 董爱华   

  1. 杭州师范学院生命科学学院, 杭州, 310036
  • 收稿日期:2003-03-13 修回日期:2003-06-21 出版日期:2005-02-10
  • 通讯作者: 贾秀英,女,1966年生,副教授.主要从事动物毒理生态学研究.发表论文20余篇.
  • 基金资助:
    浙江省自然科学基金项目资助(302306)

Effects of Pb on the peroxidase isozymes and esterase isozymes in liver and kidney of Bufo bufo gargarizans

JIA Xiuying, DONG Aihua   

  1. School of Life Sciences, Hangzhou Normal College, Hangzhou 310036, China
  • Received:2003-03-13 Revised:2003-06-21 Online:2005-02-10

摘要: 以腹腔注射法对蟾蜍(Bufo bufo gargarizans)给铅,处理7d后,使用聚丙烯凝胶垂直平板电泳法,研究了不同浓度(按铅计1、2、4、8mg·kg-1体重)的铅对蟾蜍肝脏、肾脏过氧化物酶和酯酶同工酶的影响。结果表明,不同浓度的铅均可使肝、肾组织过氧化物酶同工酶带强弱发生明显变化;对肝、肾酯酶同工酶的影响表现出一定的组织差异性,且在一定范围内主要表现为应激性,即低剂量的铅可诱导酶活性的提高。

关键词: AM真菌, 抗旱性, 保护系统, 柑橘, 嫁接苗

Abstract: Different doses of lead nitrate (1,2,4,8 mg Pb穔g-1 body weight) were applied to the adult Bufo bufo gargarizans.7 days after treatment,using vertical polyacrylamide gel electrophoresis,the effects of lead on the peroxidase isozymes and esterase isozymes in liver and kidney were studied.The results showed that the activites of peroxidase isozymes in liver and kidney were changed greatly at different concentrations of lead treatment groups.The effects of lead on esterase isozymes in liver and kidney varied with tissues,which indicated the organisms stress.It means lower dosage lead may induce esterase isozymes and improve its enzyme activity in a certain range.

Key words: AM fungi, Drought tolerance, Protective system, Citrus, Grafting seedling

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