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贵州省八个种群角倍蚜ISSR遗传多样性

王定江1;杨汉远1;钟扬2;任竹梅3   

  1. 1贵州省黔东南州林业科学研究所, 贵州凯里 556000;2复旦大学生命科学学院, 上海200433;3山西大学生命科学与技术学院, 太原 030006
  • 收稿日期:2007-12-03 修回日期:1900-01-01 出版日期:2008-10-10 发布日期:2008-10-10

Genetic diversity of eight Schlechtendalia chinensis populations from Guizhou Province: A study with inter-simple sequence repeats markers.

WANG Ding-jiang1;YANG Han-yuan1;ZHONG Yang2;REN Zhu-mei3   

  1. 1Guizhou Southeast District Forestry Institute, Kaili 556000, Guizhou, China;2School of Life Sciences, Fudan University, Shanghai 200433, China;3College of Life Science and Technology, Shanxi University, Taiyuan 030006, China
  • Received:2007-12-03 Revised:1900-01-01 Online:2008-10-10 Published:2008-10-10

摘要: 基于ISSR分子标记技术对采自贵州省8个种群共139个角倍蚜样本进行遗传多样性及其与环境因子的相关性分析。筛选的12条引物共扩增出多态性条带133条,没有种群特异带出现,平均每个引物扩增的多态带数为11条,Popgene软件分析显示,总群体多态性条带比率为100%,尽管不是特意选择变异高的引物。种群间遗传相似系数变异范围介于0.899~0.955,遗传距离介于0.046~0.106,说明角倍蚜不同种群之间的遗传分化不大。聚类分析显示,8个种群共形成2个大的聚类簇,各种群之间没有明显的地域性分布规律,但遗传多样性与3月温度和3月降水量呈负相关。

关键词: 水稻, 耐低磷, 基因型, 筛选指标, 评价指标

Abstract: Inter-simple sequence repeat (ISSR) molecular markers were used to detect the genetic diversity among 139 individuals from 8 populations of Chinese sumac aphid Schlechtendalia chinensis collected from Guizhou Province, China. 12 ISSR primers were evaluated for their ability to produce polymorphic bands, and these primers yielded a total of 133 scorable bands, averaged 11 bands each primer. There was no population-specific marker, which was present in one population but absent in the others. Popgene analysis indicated that 100% of these loci were polymorphic, even though the primers were not intentionally selected for high variability. The genetic similarity was 0.899-0.955, and the genetic distance was 0.046-0.106, indicating a lower genetic diversity of S. chinensis populations. Cluster analyses showed that the aphid populations were divided into 2 groups, and the genetic diversity had no evident correlation with geographic area but negatively correlated with the mean air temperature and rainfall in March.

Key words: Oryza sativa, Low P tolerance, Genotype, Screening index, Evaluation index