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生态学杂志 ›› 2011, Vol. 30 ›› Issue (11): 2503-2508.

• 研究报告 • 上一篇    下一篇

一株高浓度多环芳烃降解菌的鉴定和降解特性

孙翼飞1,巩宗强1**,苏振成1,金京华2,李晓军1,刘宛1,李培军1   

  1. 1中国科学院沈阳应用生态研究所, 沈阳 110016;2轻工业环境保护研究所, 北京 100089
  • 出版日期:2011-11-08 发布日期:2011-11-08

Identification and degradation characteristics of a bacteria strain degrading high concentration of polycyclic aromatic hydrocarbons.

SUN Yi-fei1, GONG Zong-qiang1**, SU Zhen-cheng1, JIN Jing-hua2, LI Xiao-jun1, LIU Wan1, LI Pei-jun1   

  1. 1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;2 Environmental Protection Research Institute of Light Industry, Beijing 100089, China
  • Online:2011-11-08 Published:2011-11-08

摘要: 采用选择性富集培养方法,从沈抚灌区土壤中分离得到多环芳烃(PAHs)高效降解菌N12,应用此降解菌制备固定化菌剂,修复焦化厂内高浓度PAHs污染土壤,并通过生理生化和16S rDNA测序进行微生物鉴定。经过30 d的降解实验,菌N12对污染土壤中各PAH的去除率>66%,总去除率为80%。生理生化和16S rDNA测序分析表明,分离得到的菌株N12为分支杆菌属(Mycobacterium sp.),该菌具有与其他分枝杆菌同源的双加氧酶基因nidApdoA2。结果表明,从土壤中筛选获得的分枝杆菌可以修复高浓度PAHs污染工业土壤。

关键词: 城市绿地系统, 景观结构, 分布格局, 环境监测, 大气污染

Abstract: By the method of selective enrichment culture, a high efficient polycyclic aromatic hydrocarbons (PAHs)-degrading bacterial strain N12 was isolated from a soil collected from Shenfu Irrigation Area, Liaoning Province, and was immobilized as a microbial agent to remedy the high concentration PAHs-polluted soil in a coking plant. Based on the physiological and biochemical analysis and 16S rRNA sequencing, the N12 was identified as Mycobacterium sp., with the isogenous dioxygenase genes nidA and pdoA2 of other Mycobacterium species. After 30 d degradation test, the removal rates of the individuals of PAHs were all >68%, and the total removal rate of PAHs was 80%, suggesting that the N12 could be used for the remediation of high concentration PAHs-polluted soil.

Key words: Urban green-land system, Landscape structure, Spatial pattern, Environmental monitoring, Air pollution