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二萜类化合物蓝萼甲素(glaucocalyxin A)对拟南芥四种磷脂酶的影响

杨鹏军,陈璐,仇奕之,安炎黄,赵敏,杨宁*   

  1. (西北师范大学生命科学学院, 兰州 730070)
  • 出版日期:2018-04-10 发布日期:2018-04-10

Effects of glaucocalyxin A on four phospholipases inArabidopsis thaliana

YANG Peng-jun, CHEN Lu, QIU Yi-zhi, AN Yan-huang, ZHAO Min, YANG Ning*   

  1. (College of Life Science, Northwest Normal University, Lanzhou 730070, China).
  • Online:2018-04-10 Published:2018-04-10

摘要: 为了探究二萜类化合物蓝萼甲素(glaucocalyxin A)对拟南芥4种磷脂酶的影响,以蓝萼甲素为供体,以哥伦比亚野生型拟南芥(Col0)和4个拟南芥磷脂酶突变体株系(pla1-1、pla2-1、plc1-1和pldδ2)为受体植物,通过施加不同浓度的蓝萼甲素(0、20、100 μmol·L-1)对5个拟南芥株系组织膜透性和渗透调节物质进行研究。结果表明:外施不同浓度的蓝萼甲素后,拟南芥5个株系中相对电导率和丙二醛(MDA)含量均显著升高,脯氨酸和可溶性蛋白含量呈现出先上升后下降的趋势,于24 h达到峰值,且不同的磷脂酶在调控其生理过程中具有不同的作用;表明蓝萼甲素作用与4种磷脂酶具有相关性,可通过调控4种磷脂酶来发挥部分化感作用。

关键词: SE-DEA, 替代效率, 影响因素, 农业化学投入强度

Abstract: The effects of different concentrations of diterpenoids glaucocalyxin A (0, 20, 100 μmol·L-1) on membrane permeability and osmotic adjustment of fiveArabidopsis thaliana lines (the ColombiaArabidopsis thaliana, and pla1-1, pla2-1, plc1-1 and pldδ2 phospholipase mutants) were examined. The results showed that the relative electrolyte leakage and malondialdehyde (MDA) contents were significantly increased by the application of glaucocalyxin A in five lines of A. thaliana. The contents of proline and soluble protein first increased and thendecreased, with a peak at 24 h. Different types of phospholipases played different roles in thephysiological regulation process. Our results suggest that the effects of glaucocalyxin A areassociated with the four types of phospholipids and that its allelopathic effects can be exerted byregulating phospholipases.

Key words: influence factor, substitution efficiency, SE-DEA, agro-chemical input intensity