铜胁迫对拟南芥幼苗生长和基因组DNA甲基化的影响

生态学杂志

铜胁迫对拟南芥幼苗生长和基因组DNA甲基化的影响

陈瑞娟^1,2,何蕾³,孙梨宗^1,2,王鹤潼¹,宋婕³,刘宛^1**

（¹中国科学院沈阳应用生态研究所，污染生态与环境工程重点实验室，沈阳 110016； ^2中国科学院大学，北京 100049； ^3辽宁大学环境学院，沈阳 11016）

出版日期:2015-09-10 发布日期:2015-09-10

Effects of copper stress on the growth and genomic DNA methylation of Arabidopsis thaliana seedlings.

CHEN Rui-juan^1,2, HE Lei³, SUN Li-zong^1,2, WANG He-tong¹, SONG Jie³, LIU Wan^1**

(¹Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ²University of Chinese Academy of Sciences, Beijing 100049, China; ^3College of Environmental Sciences, Liaoning University, Shenyang 110016, China)

Online:2015-09-10 Published:2015-09-10

摘要/Abstract

摘要： 通过Murashige and Skoog (M&S)培养实验，利用甲基化敏感扩增多态性（methylation-sensitive amplified polymorphism, MSAP）技术研究Cu²⁺胁迫对拟南芥幼苗基因组DNA甲基化水平与甲基化模式的变化，同时比较其与幼苗鲜重、根系生长对Cu²⁺胁迫的敏感性。结果表明：0、0.25、1.0 mg·L^-1 Cu²⁺处理15 d后，幼苗根长及鲜重变化差异不显著，而幼苗基因组MSAP率随Cu²⁺浓度的增加呈先增高后降低的趋势，分别为15.93%、16.28%和15.83%；高浓度Cu²⁺胁迫下（3.0 mg·L^-1），根长显著变短，鲜重显著降低，MSAP率为14.26%；Cu²⁺胁迫（0.25～3.0 mg·L^-1）下，拟南芥幼苗基因组超甲基化（M型）位点及去甲基化（D型）位点数均呈显著增加趋势，Msp I酶较Hpa II酶对胁迫反应更敏感。因此，拟南芥幼苗MSAP变化对低浓度Cu²⁺胁迫响应敏感，可作为Cu污染的早期诊断和生态风险评价。

关键词: 信号分子, 群体感应, 调控行为, 废水处理

Abstract: In this study, the changes of DNA methylation levels and patterns in Arabidopsis thaliana seedlings under 0-3.0 mg·L^-1 Cu²⁺ stress were studied using methylationsensitive amplified polymorphism (MSAP) technique, and the sensitivity of the fresh weight and root growth of seedlings to Cu stress were compared. The results showed that: (1) Under Cu stress (0.25, 1.0 mg·L^-1) for 15 days, no significant changes were observed in root growth and fresh weight of A. thaliana seedlings compared with the control group, but the genomic MSAP rate first increased and then decreased with the increase of Cu²⁺ concentration. The MSAP rates were 15.93%, 16.28% and 15.83% at 0, 0.25, 1.0 mg·L^-1 Cu²⁺, respectively; (2) At the high concentration of Cu²⁺ (3.0 mg·L^-1), the root length was shortened, the fresh weight was significantly decreased and the MSAP rate was reduced to 14.26%; (3) Under Cu stress (0.25-3.0 mg·L^-1), the number of methylation (M) type and demethylation (D) type sites increased significantly, and Msp I was more sensitive to stress response compared to Hpa II. Therefore, the MSAP of A. thaliana was sensitive to low concentration Cu²⁺ stress, which could be used for early diagnosis and ecological risk assessment of Cu pollution.

Key words: sensing signal molecule, wastewater treatment, quorum sensing, regulation behavior

陈瑞娟1,2,何蕾3,孙梨宗1,2,王鹤潼1,宋婕3,刘宛1**. 铜胁迫对拟南芥幼苗生长和基因组DNA甲基化的影响[J]. 生态学杂志.

CHEN Rui-juan1,2, HE Lei3, SUN Li-zong1,2, WANG He-tong1, SONG Jie3, LIU Wan1**. Effects of copper stress on the growth and genomic DNA methylation of Arabidopsis thaliana seedlings.[J]. cje.

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