关键词: 动物营养, 微生态学, 微生物群落

Abstract: As an indispensable part of constructed wetland, soil microbes play an important role in the material transformation and energy flow in the wetland ecosystem. This paper reviewed the research progress in the detection methods of soil microbial community, its structure and composition, and relationships of soil microbial regulation effect with environmental parameters in different type constructed wetlands. Various kinds of novel molecular biological methods have proved to be useful tools for exploring soil microbial diversity in constructed wetland, among which, denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH) were the most frequently applied methods. The regulation effect of soil microbial community in constructed wetland mainly depended on wetland hydraulic condition, wastewater property (constituent composition, and pollutants characteristics and availability), filter material or soil type, plant, and various environmental factors. The species-specific composition of soil microbial community in different type constructed wetlands was dominated by phylum Proteobacteria, followed by the members of phylums Proteobacteria, Cytophaga-Flavobacterium, Actinobacteria, and Firmicutes. How to further improve the researches on the diversity of soil microbes involving in nitrogen cycle and to increase the removal efficiency of nitrogen from wastewater would be one of the key issues for further development of constructed wetland technology.

Key words: Animal nutrient, Micro-ecology, Microbial community