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瓜多竹(Guadua amplexifolia)对NaCl胁迫的生理响应

马兰涛;陈双林   

  1. 中国林业科学研究院亚热带林业研究所, 浙江富阳 311400
  • 收稿日期:2007-12-29 修回日期:1900-01-01 出版日期:2008-09-10 发布日期:2008-09-10

Physiological responses of Guadua amplexifolia to NaCl stress.

MA Lan-tao;CHEN Shuang-lin   

  1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China
  • Received:2007-12-29 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10

摘要: 为了解瓜多竹(Guadua amplexifolia)的耐盐性,对2年生盆栽苗进行了不同浓度NaCl胁迫处理,结果表明:以处理40 d的叶片脱落率将盐害程度分为无盐害(<10%)、轻度盐害10%~50%)和重度盐害(>50%)3级;随着胁迫时间的延长,离子渗漏和MDA含量均呈上升趋势,40 d时0.30%及以上浓度的NaCl处理水平相对电导率均>50%,MDA含量显著高于低浓度处理;POD和SOD活性随胁迫时间的延长呈先升后降规律,40 d时0.20%NaCl处理的POD活性最高,0.30%NaCl处理的SOD活性最高;游离脯氨酸和可溶性蛋白等渗透调节物质也呈先升后降规律,40 d时0.30%及以上浓度的NaCl处理的可溶性蛋白和游离脯氨酸含量显著下降,0.20%及以下浓度的NaCl处理则继续上升。综合上述生理指标的反应,土壤含盐量0.30%及以上时不适合该竹种生长。

关键词: 杉木, 混交林, 土壤质量评价, 土壤功能

Abstract: A pot experiment was conducted with 2-year-old Guadua amplexifolia to study its NaCl tolerance. The results indicated that NaCl stress had significant effects on the physiological and biochemical characteristics of the plant. The salt injury was divided into 3 degrees, based on the leaf-abscission rate after 40-days exposure, i.e., noninjury (<10%), mild injury (10%-50%), and serious injury (>50%). The ion leakage and MDA content increased considerably over time, the relative conductivity of the plant exposed to 0.30% and higher salinity for 40 days was >50%, and the MDA content was higher than that at lower salinity. Both POD and SOD activities increased in the early days and then decreased with extended NaCl exposure. On the 40th day exposure, POD activity was the highest at 0.20% NaCl, while SOD activity was the highest at 0.30% NaCl. Osmoregulation substance such as free proline and soluble protein had the same trend. After 40 days exposure, the contents of free proline and soluble protein kept increasing in the plants exposed to 0.20% and lower salinity, while kept decreasing in those exposed to 0.30% and higher salinity. In summary, 0.30% and higher soil salinity was not suitable for the growth of G. amplexifolia.

Key words: Cunninghamia lanceolata, Mixed forest, Soil quality assessment, Soil function