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    10 October 2024, Volume 43 Issue 10
    Spatial-temporal variation and influencing factors of vegetation carbon use efficiency in mountain ecosystem on the northern slope of Tianshan Mountains.
    SUN Fenghua, WANG Yugang, SUN Jinjin, LI Shuangyuan, CHEN Bingming
    2024, 43(10):  2923-2931.  doi:10.13292/j.1000-4890.202410.031
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    Vegetation carbon use efficiency (CUE) is an important indicator for ecosystem carbon sequestration capacity, and for assessing the regional vegetation carbon sink capacity. Based on remote sensing images and DEM data from 2000 to 2020, we analyzed the spatial and temporal variations and influencing factors of vegetation CUE in Fukang forest region on the north slope of Tianshan Mountains. The results showed that vegetation CUE in Fukang forest region ranged between 0.42 and 0.74, with an average annual value of 0.6. CUE value was the highest in the subalpine meadow zone and the lowest in the mid-mountain forest zone. There was obvious vertical zonal differentiation, with a “parabola” distribution pattern that decreased first and then increased with altitude. The patterns of both net primary productivity (NPP) and gross primary productivity (GPP) were opposite with CUE. The regional vegetation CUE showed limited interannual variation (P>0.05). The area with significantly increased CUE only accounted for 8.72% of the total area, which was mainly distributed in the mid-mountain forest zone, indicating that longterm forest management promoted the increase of vegetation CUE in the mid-mountain forest zone. There were no significant interannual changes in vegetation CUE values between non-grazing area and grazing area (P>0.05), indicating that grazing exclusion had a weak impact on vegetation CUE in forest areas. The distribution of CUE had spatial heterogeneity in slope aspect. CUE valve was the highest in sunny slopes both in the low-mountain grassland zone and mid-mountain forest zone, and in half-sunny slope in the subalpine meadow zone. The increase of precipitation, temperature, and NDVI directly reduced CUE, while the increase of evapotranspiration indirectly reduced CUE. Natural factors had less influence on CUE in the grazing exclusion area than that in the grazing area.

    Diversity and spatial distribution pattern of woody lianas in Saraca dives community in Nonggang Nature Reserve, Guangxi
    LU Fang, WANG Bin, LI Jianxing, LI Dongxing, LIU Shengyuan, GUO Yili, WEN Shujun, HUANG Fuzhao, XIANG Wusheng, LI Xiankun
    2024, 43(10):  2932-2944.  doi:10.13292/j.1000-4890.202410.026
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    Lianas constitute a crucial component of biodiversity in tropical forests, yet the extent of their diversity and spatial distribution patterns in the north tropical karst forest remains poorly understood. In this study, we investigated the diversity and spatial distribution patterns of lianas within a typical Saraca dives community in the northern tropical karst seasonal rainforest of Nonggang, Guangxi. We elucidated the diversity distribution patterns of lianas and explored the disparities in spatial distribution patterns among distinct taxonomic groups based on 100 quadrats, each measuring 10 m×10 m in a 1 hm2 plot. We found that: (1) In the 1 hm2 Saraca dives community, 52 species of woody lianas were identified, belonging to 25 families and 43 genera. They represented 55.32% of the total woody species. Furthermore, the Simpson index for lianas was 0.909, the Shannon index was 2.842, and the Pielou evenness index was 0.723. These indices collectively indicate a relatively high level of diversity within the community. There was clear presence of dominant and endemic species. Fabaceae and Vitaceae families stood out for their remarkable species richness and abundance, respectively. Tetrastigma planicaule was the dominant species, with the highest importance value. Furthermore, endemic species constituted 34.61% of the total species number. (2) Lianas demonstrated an aggregated distribution within the spatial scale ranging from 0 to 25 m. Upon removing habitat heterogeneity via the heterogeneous Poisson null model, lianas tended towards a random distribution pattern as the spatial scale increased. When habitat heterogeneity was eliminated, over 70% of liana species exhibited varying patterns of random distribution across specific spatial scales. However, at finer scales, these species still exhibited aggregated distributions. These findings suggest that both habitat heterogeneity and dispersal limitations may influence the spatial distribution patterns of lianas. The observations suggest that both habitat heterogeneity and dispersal limitations may influence the spatial patterns of lianas. Furthermore, there was a negative correlation between the aggregation of lianas and their abundance, as well as their average and maximum diameters at breast height. Notably, lianas with a climbing efficiency of 2 (stem twining) exhibited a higher degree of aggregation. The community of Saraca dives exhibited remarkable diversity in lianas, served as a crucial component of the community. Additionally, the spatial distribution pattern of lianas was not solely related to habitat heterogeneity, but was also influenced, to some extent, by intrinsic attributes such as climbing efficiency and mode. This study lays a crucial foundation for examining the maintenance mechanisms of species diversity in typical forest communities, as well as assessing the impact of lianas on forest carbon storage and dynamic processes.

    Comparison on growth performance, wood properties, and morpho-physiological characters among three poplar species in western Songnen Plain.
    HU Yanbo, ZHANG Peng, ZHANG Zheng, REN Zhanqi, WANG Fusen, XU Huadong, XING Zhenghua, ZHANG Xiuli, ZHAO Xiyang, DING Changjun
    2024, 43(10):  2945-2952.  doi:10.13292/j.1000-4890.202410.027
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    Aiming to optimize site-species matching in western Songnen Plain, we compared growth performance, wood properties, and morpho-physiological characters of 15-year-old Populus simonii × P. nigra (XH), P. euramericana × P. ussuriensis (HQ) and P. alba × P. berolinensis (YZ). The results showed that height and diameter at breast height of YZ and HQ were significantly greater than that of XH (P<0.05), with the timber volumes being 3.5 and 2.8 times of XH (0.08 m3·individual-1), respectively. Wood absolute water content followed an order of HQ (140%) > HX (110%) > YZ (73%), whereas the absolute dry density followed an order of YZ (0.45 g·cm-3) > HX (0.42 g·cm-3) > HQ (0.38 g·cm-3). Wood pH and hemicellulose content rather cellulose and lignin content differed significantly among the three species. There were significant differences in xylem anatomy and branch-leaf morphological characters among the species, with YZ having the highest tree-ring width, vessel number, theoretical water conductivity, branch basal area/length ratios and leaf area but the lowest crown width and fresh-weight ratios of leaves and branches. Generally, the concentrations of N, P and K in leaves were significantly higher than that of branches (P<0.05) for the three species. YZ had significantly higher concentrations of N, chlorophyll, free amino acids, but lower concentrations of NO3--N, NH4--N, and ratios of C/P (P<0.05) in leaf, than the other two species. HQ had significantly higher concentrations of non-structural carbohydrates and ratios of C/N and C/K in leaf than the other species. YZ had significantly higher leaf photochemical efficiency than XH and higher leaf ratios of N/K than HQ. In summary, YZ can be preferentially selected for planting in western Songnen Plain. However, in the perspective of wood properties, HQ may not be an appropriate species in comparison with XH.


    Impact of warming on the composition of three plant communities in alpine tundra of the Changbai Mountains.
    GU Yongfeng, HAN Yingying, JIN Yinghua, XU Jiawei, ZHANG Huihui
    2024, 43(10):  2953-2959.  doi:10.13292/j.1000-4890.202410.025
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    Alpine tundra are sensitive and vulnerable to global climate change. With open-top chambers (OTCs) in alpine tundra of the Changbai Mountains, we compared the responses of community compositions of tundra invaded by various degrees of Deyeuxia angustifolia to warming, aiming to understand the possible changes of plant community compositions under global warming. Our results showed that the average air temperature of the OTCs increased by 2.1 ℃ during the growing season, which can simulate the amplitude of temperature increase in the next century. Soils of different plant communities differed in their response to experimental warming. During the growing season, surface soil temperature of the mixed Rhododendron chrysanthum-Vaccinium uliginosum community, R. chrysanthum-D. angustifolia community and D. angustifolia-R. chrysanthum community increased by 2.13, 1.00 and 1.06 ℃, respectively. Those results indicated that soil temperature of the native plant community increased significantly and the changes of plant community compositions could effectively inhibit soil warming, resulting in reducing ecological response to warming. After 3-year continuous warming, the composition of the three plant communities changed. The importance values of D. angustifolia significantly increased in the R. chrysanthum-D. angustifolia community and D. angustifolia-R. chrysanthum community. In contrast, the importance value of R. chrysanthum decreased, especially in R. chrysanthum-D. angustifolia community and R. chrysanthum-V. uliginosum communitiy. Experimental warming inhibited native species, but promoted D. angustifolia encroachment. Warming had minimal effects on the colonization of D. angustifolia, whereas had important influence on its growth and expansion. Along with the invasion of D. angustifolia, vegetation changes were more notable in response to warming. The Simpson index, Shannon index and Pielou index under warming treatment were lower than under control treatment in the three plant communities. Similarly, species diversity decreased in plant communities, with the obvious decline in the D. angustifolia-R. chrysanthum community. Therefore, climate warming led to biodiversity decline of alpine tundra communities.

    Radial growth response of Larix principis-rupprechtii to climate change in Xiaowutai Mountain.
    WANG Hui, LIU Chunlan, QIAO Qing, ZHANG Wentao, NING Yangcui, DONG Manyu
    2024, 43(10):  2960-2966.  doi:10.13292/j.1000-4890.202410.019
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    We analyzed the response and stability of tree radial growth of Larix principis-rupprechtii , a dominant conifer species in Xiaowutai Mountain, to climate factors based on the ring-width chronologies at different altitudes. The variations of radial growth of L. principis-rupprechtii under the background of climate warming were evaluated. The results showed that: (1) The chronologies of L. principis rupprechtii were similar at low (1380 m), medium (1755 m), and high altitude (2080 m) on the northern slope of Xiaowutai Mountain. The three chronologies had good quality and were suitable for dendroclimatological study. (2) Precipitation was the main limiting factor for the radial growth of L. principis-rupprechtii. Radial growth was positively correlated with precipitation in September of the previous year (P<0.05), the temperature in February (P<0.05) and May (P<0.01) of the current year. (3) After the abrupt change of temperature in 1993, the positive relationship between chronology and precipitation in May was relatively stable (P<0.01). However, the negative relationship between radial growth and growing season temperature was enhanced, especially in May (P<0.05). This may result mainly from water stress caused by the rising temperatures. Therefore, with the intensification of warming and drying in the future, the radial growth rate of L. principis-rupprechtii may show a downward trend. At present, the radial growth-climate relationship of L. principis-rupprechtii in Xiaowutai Mountain is generally stable.

    Effects of litter quantity on soil enzymes and stoichiometric ratios of spruce plantations with different stand densities in the subalpine region of western Sichuan.
    LI Huichao, PAN Hongli, FENG Qiuhong, MA Wenbao, DENG Dongzhou
    2024, 43(10):  2967-2978.  doi:10.13292/j.1000-4890.202410.024
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    Changes in the input of leaf litter can affect soil enzyme activity and stoichiometric ratios by changing soil biochemical properties. However, it is not yet clear whether the magnitude of the effects of changes in leaf litter input on soil enzyme activity and stoichiometric ratios differed across stands with different densities. With an indoor simulated experiment with four treatments (leaf litter removal, 50% leaf litter reduction, control, and double leaf litter addition), we examined the effects of leaf litter quantity on soil biochemical properties, extracellular enzyme activity, and stoichiometry ratio of high-density and low-density spruce plantations in the subalpine of western Sichuan. The results showed that leaf litter double addition significantly decreased soil organic carbon content of the high-density stand, as well as the total dissolved nitrogen and nitrate nitrogen contents of the two stands, but signi-ficantly increased soil microbial biomass carbon and nitrogen of the two stands. Compared with litter removal and reduction treatments, leaf litter double addition significantly reduced β-glucuronidase activity and acid phosphatase activity in both stands. However, soil β-glucosidase was more sensitive to leaf litter double addition treatment in the low-density stand, showing that the soil β-glucosidase activity under leaf litter double addition treatment decreased by 28.6%. With increasing leaf litter input, the ratio of enzyme nitrogen to phosphorus (0.69-0.65), the ratio of enzyme carbon to nitrogen (0.50-0.44), and the ratio of enzyme carbon to phosphorus (0.34-0.28) showed a decreasing trend. The soil nitrogen, phosphorus, and enzyme carbon-nitrogen ratios in the high-density stand were significantly lower than those in the low-density stand. The results of RDA analysis showed that soil total nitrogen, ammonium nitrogen, total phosphorus, and dissolved organic carbon were the main factors affecting soil enzyme activity and stoichiometric ratios. In summary, soil nitrogen limitation of the high-density stand is more evident than that of the lower-density stand, and both stands gradually changed from nitrogen limitation to phosphorus limitation with the increases of leaf litter input.

    Elevational patterns and environmental drivers of soil organic carbon content and storage in a warm-temperate forest.
    ZHANG Xiongzhi, ZHANG Huifang, WANG Chenlin, REN Yuchen, WANG Xinyue, WANG Jingjing, CHEN Song, XU Maohong, YANG Xiuqing, SONG Houjuan
    2024, 43(10):  2979-2987.  doi:10.13292/j.1000-4890.202410.011
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    Exploring soil organic carbon (SOC) changes along environmental gradients in climate transition zone is essential for predicting soil organic carbon dynamics under climate change. To understand the spatial variations of SOC content and storage in the transition zone of subtropical to warm-temperate climate, 15 monitoring forest plots were set up in Lishan National Nature Reserve in Shanxi Province along an elevation gradient from 890 m to 2200 m above sea level at a 100 m interval. We assessed the elevational changes in SOC content and storage and their relationships with plant diversity, soil physicochemical and climatic factors, and microbial characteristics by using five-point sampling method. The results showed that both SOC content and storage showed overall U-shaped trends along the elevational gradient. The SOC content and storage peaked at 1645 m (75.41±14.05 g·kg-1) and 894 m (51.85±9.89 Mg·hm-2), respectively. Results of the Mantel test showed that SOC content was significantly correlated with soil factors, climatic factors, and microbial species richness, while the spatial variation in SOC storage was only significantly correlated with soil factors and microbial species richness. Environmental factors explained 64.7% and 33.9% of the total variations of elevational patterns in soil SOC content and storage, respectively. Annual precipitation was the largest predictor (14.8% of total explained variance) for SOC content, followed by NO3--N (13.4%) and  annual mean temperature (13.2%). For SOC storage, soil total phosphorus had the strongest effect with over 15% of total explained variance, followed by NO3--N (8.7%) and  annual mean temperature (7.5%). This study provides an important reference for evaluating soil organic carbon storage and its variation in forests in the climatic transition zone.

    Comparative analysis of soil microbial composition of four typical plant communities in Momoge National Nature Reserve, Jilin Province.
    LIU Weixuan, LI Yimeng, JIANG Hongxing, WANG Yihua, CHEN Lixia, WANG Ying
    2024, 43(10):  2988-2998.  doi:10.13292/j.1000-4890.202410.040
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    As one of the important components of wetland ecosystems, soil microorganisms are the main driving force of decomposition of animal and plant residues, playing an important role in nutrient cycling and maintaining soil physicochemical properties. To explore the composition of soil microorganisms in the wetland of Momoge National Nature Reserve in Jilin Province, soil microbial communities and soil physicochemical properties were analyzed under four typical wetland plant communities, which were dominated by Suaeda glauca, Scirpus planiculmis, Scirpus nipponicus, and Phragmites australis, respectively. The results of 16S rRNA and ITS fulllength sequencing showed that Proteobacteria was the most abundant bacterial phylum in the soils of all the four plant communities, while Ascomycota was the most abundant fungal phylum. Richness and diversity of soil bacteria were the highest in the two Scirpus dominated communities, while soil fungi richness was the highest in S. nipponicus community. However, there was no significant difference in soil fungi diversity among the four plant communities. LEfSe analysis showed that the relative abundance of Bacteroidales, Clostridia, Clostridiales, and Alternaria in the soil of S. planiculmis community was significantly higher than that in the other three plant communities. The relative abundance of Proteobacteria, Acidobacteria, Rozellomycota, Pseudomonas, and Lepiota in the soil of S. nipponicus community was significantly higher than that of the other three plant communities. The composition and diversity of soil bacterial communities were greatly affected by soil pH, electrical conductivity, and organic carbon concentration. Our results provide reference for the maintenance of wetland ecological functions and the improvement of soil microbial community structure in Momoge National Nature Reserve.

    Soil microbial biomass carbon, nitrogen, phosphorus contents and their ecological stoichiometric characteristics along an elevation gradient in the Wuyi Mountains.
    CHEN Jieni, SHI Siyu, ZHONG Xianfang, SI Youtao, MA Hongliang, GAO Ren, YIN Yunfeng
    2024, 43(10):  2999-3004.  doi:10.13292/j.1000-4890.202410.004
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    The study of soil microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), microbial biomass phosphorus (MBP) and their ecological stoichiometry along an elevation gradient is of great significance for understanding soil nutrient cycling and its relationship with environmental changes in subtropical mountains. In this study, soil samples were collected along an elevation gradient (350, 650, 950, 1050, 1850, and 2100 m) in the Wuyi Mountains to investigate elevational variations of the contents of MBC, MBN, MBP and the ratios of MBC/MBN, MBC/MBP, MBN/MBP. The results showed that soil MBC and MBN increased with elevation, while MBP and the ratios of MBC/MBN, MBC/MBP and MBN/MBP remained stable. MBC, MBN, and MBP were significantly positively correlated with the contents of soil total carbon (TC), total nitrogen (TN), available phosphorous (AP), dissolved organic carbon (DOC), dissolved organic nitrogen (DON), and soil water content (SWC). Soil MBC/MBN had a significantly positive correlation with soil DON, NO3--N, NH4+-N contents and a significantly negative correlation with soil pH. Soil MBC/MBP was positively correlated with soil TN, DOC, TC, DON, SWC contents, and negatively correlated with soil C/N. Soil MBN/MBP was only positively correlated with soil pH. Results of redundancy analysis further indicated that soil TC content was the key factor influencing the contents and stoichiometry of MBC, MBN, and MBP.

    The distribution characteristics and influencing factors of soil bacterial diversity in cultivated land at the county scale in the Sanjiang Plain
    ZHU Jianning, LIU Jie, WANG Changkun, GUO Zhiying, MA Haiyi, HAO Shilong, PAN Xianzhang
    2024, 43(10):  3005-3014.  doi:10.13292/j.1000-4890.202410.035
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    The distribution of soil microbial diversity and its influencing factors have attracted much attention. Many studies at large spatial scales including global, continental, or national levels, have found that climate and soil properties are the main influencing factors. However, the distribution characteristics and influencing factors of soil bacterial diversity at medium and small spatial scales such as the county scale still need to be further clarified. To address this issue, 98 soil samples were collected from Youyi County, located in the Sanjiang Plain, Heilongjiang Province, China, and high-throughput sequencing and bioinformatics were performed to quantify bacterial diversity. Results showed that soil physicochemical properties and bacterial community composition differed significantly between paddy field and dryland, with soil bacterial α diversity being significantly higher in paddy field than in dryland. Soil pH, organic matter, total nitrogen, and total potassium were the main factors influencing soil bacterial α diversity and the relative abundance of dominant phyla in cultivated land. Soil bacterial community structures were also significantly different between paddy filed and dryland. Results of the Mantel test showed that soil bacterial community structure was primarily affected by soil pH and fertility factors, and as well as by geographic distance. When paddy field or dryland was considered separately, soil total phosphorus did not affect bacterial community structure in paddy field, and soil total phosphorus and geographic distance had no significant effects in dryland either. Empirical Bayesian kriging interpolation results showed that soil bacterial α diversity was higher in the northeast than in the southwest of the study area. In summary, land use and soil properties are the main factors affecting soil bacterial diversity and community structure at county level. The overall distribution trend of bacterial diversity in the study area is high in the northeast and low in the southwest. Our results would provide reference for soil health assessment of cultivated land at the county scale.

    Change of fungal community structure in fluvo-aquic soil under different CO2 concentrations
    SI Yakun, FENG Biao, NIU Yinxing, WANG Yi, LI Hui, LI Peipei, LI Fang, HAN Yanlai
    2024, 43(10):  3015-3022.  doi:10.13292/j.1000-4890.202410.006
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    Agricultural production is influenced by atmospheric CO2 concentration. Fungi are the key promoters of organic matter degradation and nutrient cycling in soil, but their responses to the increase of CO2 concentration are not clear. Miseq high-throughput sequencing technology was used to investigate the fungal community structure under four CO2 concentrations (ambient, 2%, 4%, 6%) in microcosm experiments on typical fluvo-aquic soil in North China Plain. The results showed that fungal α diversity decreased significantly at 4% and 6% CO2 concentration. Ascomycota, Basidiomycota, Mortierellomycota dominated the fungal community. The relative abundance of Basidiomycota and Glomeromycota decreased with the increases of CO2 concentration, while that of Ascomycota increased. The dominant genera included Mortierella, Fusarium, Citripora, Stachybotrys, and Humicola. The relative abundance of Citripora decreased with the increases of CO2 concentration, but that of Mortierella increased twofold in the condition of 2% CO2 concentration. CO2 concentration positively correlated with the relative abundance of Humicola phialophoroides and Sarocladium hominis, but negatively correlated with the relative abundance of Citripora afrocitrina. The fungal network topology in 2% CO2 treatment was similar to the control, but with more edges. The network topology changed obviously in 4% and 6% CO2 treatments, differentiating into two network modules. The keystone taxa were Aspergillus terreus, Trichoderma yunnanense, and Spizellomyces sp. This study clarified the response of fungi to different CO2 concentrations in fluvo-aquic soil, and provided theoretical reference for studying the impacts of elevated CO2 on ecosystem functions of farmland.

    Effects of different modified materials and their combinations on photosynthesis and root morphological characteristics of maize in saline-alkali soil of Hetao Plain.
    BAI Xiaolong, WU Jinmin, ZHANG Jiahao, WANG Teng, YANG Zhenghu, TIAN Feng, ZHAO Hui, WANG Bin
    2024, 43(10):  3023-3030.  doi:10.13292/j.1000-4890.202410.015
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    To explore the effects of different modified materials and their combined applications on the morphological characteristics of maize at seedling stage, an experiment was conducted on the saline-alkali land in Hetao Plain of Inner Mongolia. There were eight treatments, including control (CK), desulfurized gypsum (T1), soil ameliorant (T2), organic fertilizer (T3), desulfurized gypsum combined with soil ameliorant (T4), desulfurized gypsum combined with organic fertilizer (T5), soil ameliorant combined with organic fertilizer (T6), and desulfurized gypsum combined with soil ameliorant and organic fertilizer (T7). We measured plant height, photosynthetic rate and root morphological characteristics of maize seedlings. The results showed that: (1) the T7 treatment had the strongest promoting effect on plant height, stem diameter and SPAD value, with an increase of 30.0%, 24.2% and 27.8%, respectively. (2) The combined treatments significantly increased net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and leaf water use efficiency, but significantly decreased transpiration rate. The combined treatments had a better promoting effect on root morphological characteristics (total root length, surface area, number, volume) than the single treatments, with a significant increase in root branching and a significant increase in the number of lateral roots and root hairs with a diameter less than 1 mm. (3) Principal component analysis showed that the scores of maize photosynthetic performance and root morphological characteristics for different treatments were T7>T4>T6>T5>T2>T3>T1>CK. In conclusion, compared with single application and combined applications of two modified materials, the combined application of three modified materials had a stronger promoting effect on leaf photosynthetic capacity and root morphological characteristics of maize seedlings.

    Effects of longterm ricecrayfish rotation on soil bacterial community structure.
    PENG Liang, DAI Lili, TAO Ling, ZHANG Hui, ZHU Jianqiang, LI Gu
    2024, 43(10):  3031-3039.  doi:10.13292/j.1000-4890.202410.012
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    We investigated the impacts of long-term rice-crayfish rotation on physicochemical properties and bacterial community structure in paddy soils. Chemical analysis methods and high-throughput sequencing of 16S rRNA amplicons were employed to examine the soil physicochemical properties and bacteria community structures in rice monoculture and rice-crayfish rotation systems with durations of 4, 8, and 21 years. Results showed that ricecrayfish rotation significantly increased total phosphorus content by 37.01%-54.33% (P<0.05), but decreased available phosphorus content by 60.21%-61.10% (P<0.05) compared to rice monoculture. Results of principal coordinate analysis and multivariate analysis of variance showed significant differences in soil bacteria community structure across systems with different rotation years (P=0.004). Long-term rice-crayfish rotation increased the relative abundance of Aminicenantia (family, order, class, genus) and Sva0485 (family, order, class, genus) in paddy soil. Redundancy analysis (RDA) results identified organic matter and available nitrogen as key factors influencing bacterial community structure. Overall, rice-crayfish rotation affected soil phosphorus levels and altered the abundance of bacterial phyla, without impacting community diversity in soil bacteria. Our results provide valuable insights into the impact of rice-crayfish rotation on paddy soil bacterial communities.

    Effects of low temperature and excessive precipitation at seedling stage on soybean yield in high-latitude cold region.
    QU Huihui, LI Xiufen, ZHU Haixia, WANG Liangliang, QU Bingyang, WANG Qiujing, LYU Jiajia, JI Yanghui, JIANG Lixia
    2024, 43(10):  3040-3046.  doi:10.13292/j.1000-4890.202410.003
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    The effects of low temperature and rainy weather at seedling state on soybean yield were examined to provide basis for the prevention and reduction of soybean disaster. The localization and adaptability of WOFOST model were firstly tested based on the observation data of soybean growth in Heilongjiang Province and the results of previous research. Using daily meteorological data, regional crop parameters and soil parameters from 15 representative stations in the study area during 1971-2020, soybean yield under different low temperature and rainy scenarios was simulated, and the influence of low temperature and rainy weather at seedling stage on soybean yield was quantitatively evaluated. The results showed that the localization application of WOFOST model was effective in Heilongjiang Province and thus it could be used to simulate soybean yield in this region. Under the scenarios set here, low temperature, rainy weather and their combined effects would lead to significant reductions of soybean yield. The duration of combined effects was the main factor leading to soybean yield reduction. The t-3.5 scenario lasting for 6 days had no significant effect on soybean yield, while some cases of t-3.5 scenario lasting for 12 days and most cases of t-3.5 scenario lasting for 18 days significantly reduced soybean yield. Under the context of the same duration and degree of agro-disaster, the combined occurrence of low temperature and heavy rain had stronger effect on soybean yield than that of the two alone.

    Mechanism underlying the improvement of stress resistance and quality in Brassica chinensis by alkaline thermal hydrolysate of sewage sludge.
    BAI Ju, LIU Xiaolin, XUE Xiaorong, WU Chenrui, GUO Caixia, MENG Qiuxia, YANG Zhiping, ZHANG Qiang, WANG Yongliang
    2024, 43(10):  3047-3054.  doi:10.13292/j.1000-4890.202410.007
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    Abiotic stresses such as drought and salinity are the major environmental factors hindering plant growth and development. The discharge of municipal sewage sludge has been increased in recent years. The sewage sludge can be treated using alkaline thermal hydrolysis technology. The product alkaline thermal hydrolysate (ATH) contains abundant calcium and organic nitrogen nutrients. To investigate the effects of ATH on the resistance of vegetables against environmental stresses and accomplish the recycling of sewage sludge, Brassica chinensis was subjected to three stress treatments (no stress, drought stress, and salt stress) and three fertilization treatments (no fertilizer, ATH, and urea) in a pot experiment. The results showed that the application of ATH enhanced the yield of B. chinensis by 43.3% compared with urea application without abiotic stress. ATH application reduced nitrate content and increased the contents of soluble sugar, soluble protein and Vc in B. chinensis. Under drought stress and salinity stress, ATH application increased the contents of water-soluble calcium and exchange calcium in the soil and increased the calcium uptake of B. chinensis. ATH promoted the accumulation of proline and decreased the content of malondialdehyde. Furthermore, ATH significantly increased the activity of catalase by 8.6% under drought stress and the activity of peroxidase by 70.4% under salt stress, and thus alleviated the stress caused by adverse environments. The application of ATH to vegetable production can reduce fertilizer input, improve yield, quality and stress resistance of crops, and consequently is a new way for effective, harmless and resource utilization of municipal sewage sludge.

    Growth rhythm of three landscape plant species planting on municipal sewage sludge.
    PENG Yuanhang, FENG Jiayi, LONG Fengling, ZENG Shucai
    2024, 43(10):  3055-3065.  doi:10.13292/j.1000-4890.202410.032
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    We explored the variations of seedling height and ground diameter growth rhythm and biomass of Brachychiton acerifolius, Koelreuteria bipinnata and Heteropanax fragrans on municipal sewage sludge, aiming to provide reference for the resource utilization of sewage sludge in landscape greening. In a 1-year pot experiment, we measured height and ground diameter of seedlings of the three species planting on red soil (CK), sludge-red soil substrate (mass ratio 50%, S50), and 100% sludge (S100). Logistic equation was used to establish the model of plant growth. Growth rhythm and biomass of three species on different substrates were comparatively analyzed. The results showed that: (1) Compared with CK, S50 and S100 significantly increased height of B. acerifolius, height and ground diameter of K. bipinnata, and ground diameter of H. fragrans. S50 and S100 also increased maximum monthly growth and total increment of height and ground diameter of H. fragrans. The maximum monthly growth of height and ground diameter of K. bipinnata was lower than that of B. acerifolius and H. fragrans. (2) The Logistic growth model fitting effect of height and ground diameter reached extremely significant level (P<0.01). The growth phases of plant height and ground diameter were divided into early growth phase, rapid growth phase, and late growth phase. (3) Root biomass, stem biomass, leaf biomass and quality index of H. fragrans were higher in S50 and S100 than in CK. H. fragrans had the highest quality index (14.20) in S50 among the three species, and the quality index of H. fragrans and B. acerifolius in S100 treatment was significantly higher than that of K. bipinnata. According to the growth performance, H. fragrans had the best sludge adaptability, followed by B. acerifolius and K. bipinnata. The Logistic growth model could better predict and analyze the growth pattern at seedling stage, which reflected the growth response of different plant species to sewage sludge.

    Effects of amendment-plant combined remediation on the physicochemical property, enzyme activity and microbial community structure of bauxite residue.
    CHEN Yulu, LUO Youfa, WAN Zuyan, WAN Qiansong, ZOU Yuzheng, WU Yonggui
    2024, 43(10):  3066-3075.  doi:10.13292/j.1000-4890.202410.030
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    Amendment application combined with vegetation restoration is an economical and effective approach to improve the hostile habitat conditions of bauxite residue sites. In this study, different compound amendments (e.g., wood vinegar, phosphogypsum, mushroom residue, sewage sludge, cow manure, and fish manure) and ryegrass (Lolium perenne) were used as experimental materials for phytoremediation of bauxite residue. A 90-day pot experiment was conducted to investigate the effects of amendment-plant combined remediation on the physicochemical property, enzyme activity, and microbial community structure of bauxite residue. The results showed that single application of wood vinegar and phosphogypsum could regulate the alkalinity of bauxite residue, with pH being decreased by 4.1% and 11.4%, respectively. However, lower nutrient availability hindered the stable growth of plants. The amendment-plant combined remediation decreased pH and exchangeable sodium percentage of bauxite residue from 9.8 to 7.6-7.8 and from 29.3% to 25.9%-28.2%, respectively. Additionally, the amendment-plant combined remediation significantly increased nutrient contents (available nitrogen, available phosphorus, total nitrogen, total phosphorus, and organic matter), enzyme activities (urease, catalase, alkaline phosphatase, and sucrase), and diversity and relative abundance of microbial communities in bauxite residue. The dominant bacterial phyla were Proteobacteria, Actinobacteriota, Bacteroidota, and Firmicutes, while the dominant fungal phylum was Ascomycota. Overall, the amendment-plant combined remediation significantly improved physicochemical property, enzyme activity, and microbial community structure of bauxite residue, which is of great significance in comprehensively indicating the soil formation processes of bauxite residue.

    Effects of different amendments on fungal community structure and functional groups of Eucalyptus camaldulensis rhizosphere in Cd and Pb tailings.
    YANG Jiaqi, OUYANG Linnan, CHEN Shaoxiong, ZHANG Cheng, ZHENG Jiaqi, HE Shae
    2024, 43(10):  3076-3085.  doi:10.13292/j.1000-4890.202410.036
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    To clarify the remediation effects of four amendments (bacteria-based organic manure, organic manure, inorganic fertilizer and bacterial agent) separately combined with Eucalyptus camaldulensis planting on Cd and Pb tailings, we examined physicochemical properties and the proportions of different forms of Cd and Pb in the tailings. Fungal community structure and functional groups were analyzed based on Illumina high-throughput sequencing technology and FUNGuild platform. The dominant environmental factors regulating fungal community structure were analyzed. The results showed that organic manure and bacteria-based organic manure combined with E. camaldulensis significantly increased pH, cation exchange capacity and the proportion of residual Pb while significantly decreased the proportion of reducible Pb. The high-throughput sequencing results showed that the four amendments significantly changed fungal community structure, increased the relative abundance of Agaricomycetes and reduced that of Sordariomycetes in tailings when combining with E. camaldulensis. Functional group analysis results showed that, when combining with E. camaldulensis, organic manure and bacteria-based organic manure increased the relative abundance of soil saprotroph and reduced that of plant pathogen. By contrast, inorganic fertilizer and bacterial agent reduced the relative abundance of soil saprotroph and increased that of plant pathogen. The results of correlation analysis and Mantel test showed that cation exchange capacity, pH, and the proportion of different forms Pb were the key factors affecting fungal community structure. Our results suggest that organic manure and bacteria-based organic manure combining with E. camaldulensis can increase pH and cation exchange capacity, reduce the biological toxicity of Pb in tailings, change the fungal community structure, increase the proportion of soil saprotroph, and reduce plant pathogens. These two amendments are ecologically safe methods for the remediation of Cd and Pb tailings.

    Spatial variability and influencing factors of soil available microelements in the source region of the Danjiang River.
    ZHANG Yafeng, WANG Yingwei, QIAN Xinyu, YANG Yunjun, YOU Jun, HE Yuanfang
    2024, 43(10):  3086-3094.  doi:10.13292/j.1000-4890.202410.001
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    Geostatistics, correction analysis, regression analysis and GIS technology were used to clarify the spatial variations of soil available microelements (Fe, Mn, Cu, Zn and B) and to explore their influencing factors in different geological formation units in the source region of the Danjiang River of the Middle Route of China’s South-to-North Water Transfer Project. The results showed that the average contents of soil available Fe, Mn, Cu, Zn and B were 45.51, 42.39, 1.26, 3.34 and 0.39 mg·kg-1, respectively. Among them, the contents of available Fe, Mn and Zn were above the rich levels, available Cu reached the medium level, while available B was at the poor level. According to the results of semi-variance analysis, soil available Zn exhibited strong spatial variability (CV=130.4%) in different geological formations, influenced by structural factors. Soil available Mn, B, Fe and Cu all showed moderate spatial variabilities (CV=52.8%-94.2%), which were mainly affected by both structural and random factors. Soil available N, P, K and organic matter contents were positively correlated with soil available microelements, while soil pH was negatively correlated with available Mn and Fe, but not significantly correlated with available B, Cu and Zn. Generally, the geological formation has a strong explanatory power on the spatial variation of soil available microelements, which provides a reliable reference for the utilization and management of land resources in different regions based on geological formations.

    Effect of freeze-thaw process on the synchronous stabilization of cadmium and arsenic in soil by ferromagnetic biochar.
    CHEN Laiyi, LI Xiaojun, WANG Yi, HOU Wei, GONG Zongqiang, JIA Chunyun
    2024, 43(10):  3095-3103.  doi:10.13292/j.1000-4890.202410.005
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    Understanding the impacts of freeze-thaw processes on the long-term stabilization of heavy metals in polluted soil in cold regions of northern China is an important basis for screening remediation techniques. In this study, we examined the effects of freeze-thaw frequency and water content on the synchronous stabilization of cadmium and arsenic in soil by ferromagnetic biochar (F-MBC) in the freeze-thaw process, taking the soil contaminated by in situ heavy metals for many years as the object. The results showed that as the frequency of freeze-thaw increased, soil pH and electrical conductivity showed a trend of first increasing and then decreasing, but both were higher than that before freeze-thaw. Under the same freeze-thaw frequency, the increase in initial water content did not have a significant impact on soil pH under F-MBC treatment, but soil electrical conductivity showed a downward trend. Compared with those before freeze-thaw, the contents of available Cd and As in soil experienced a pattern of first increasing and then decreasing during the freeze-thaw process, but both were significantly higher than those before freeze-thaw in the soil. With the increases of initial water content, soil available Cd content continued to decrease and was significantly lower than that in the control (non-freeze-thaw). Available As content first increased and then decreased, and was higher than that in the control (non-freeze-thaw). Our results demonstrate that F-MBC can still achieve synchronous and stable remediation of Cd and As contaminated soil after the freeze-thaw process, but the risk of As pollution would increase. These results provide theoretical support for optimizing the stabilization and remediation technology of cadmium and arsenic composite contaminated soil in cold regions.

    Spatiotemporal differentiation and variation trend of drought in winter wheat in the Huang-Huai-Hai region from 1981 to 2020.
    ZHAO Junfang, XIE Hongfei, YANG Jiaqi, PENG Huiwen, HUANG Ruixi, HUO Zhiguo, MA Yuping
    2024, 43(10):  3104-3112.  doi:10.13292/j.1000-4890.202410.008
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    Multi-source data of meteorology, disaster, crop, and soil in the cultivation areas of winter wheat in the Huang-Huai-Hai region from 1981 to 2020 were used to determine the water deficit indices at different development stages of winter wheat based on the reference crop evapotranspiration and water demand. Combined with the drought classification standard of winter wheat, the spatial-temporal variations of droughts in winter wheat in the Huang-Huai-Hai region were explored, which was of great significance for disaster prevention and reduction. The results showed that droughts in winter wheat from 1981 to 2020 existed obvious temporal and spatial variations in the Huang-Huai-Hai region. The areas affected by severe drought decreased, while the areas affected by moderate drought significantly increased. For the whole growth period of winter wheat, the most serious disaster during 1981-2020 was severe drought in the Huang-Huai-Hai region, followed by moderate drought, and finally light drought. The drought level gradually weakened from the north to the south in space, with a striped distribution. The winter wheat growing regions of 35°N northward and of 120°E eastward were basically suffered from serious drought all year round. For the growth period before winter, the most serious disaster during 1981-2020 was severe drought, followed by moderate drought and mild drought. The areas experiencing severe drought significantly decreased, while that affected by moderate drought significantly increased during the growth period before winter. For the overwintering period, the winter wheat during 1981-2020 in the Huang-Huai-Hai region suffered the extreme drought, followed by severe drought, and finally moderate drought. The areas affected by severe drought obviously decreased, while the areas affected by moderate drought increased during the overwintering period of winter wheat. For the period of regreening and heading, the droughts in winter wheat during 1981-2020 could be divided into four levels: mild drought, moderate drought, severe drought, and extreme drought. The areas with severe drought significantly decreased, while the areas with extreme drought significantly increased during the regreening and heading period of winter wheat. The droughts of winter wheat during the stage of grain filling and mature from 1981 to 2020 were mainly divided into three levels: no drought, mild drought, and moderate drought. The winter wheat areas with moderate drought were significantly decreased, while areas with mild drought increased during the stage of grain filling and mature.

    Distribution characteristics of flux source of rice-wheat rotation agroecosystem in Huaihe River Basin.
    ZHNAG Kaidi, YAO Yun, LING Xinfeng, YAN Shaowei, ZHANG Fangmin, LU Yanyu
    2024, 43(10):  3113-3120.  doi:10.13292/j.1000-4890.202410.002
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    Flux footprint models are often used to explain the sources of flux data from flux tower measurements, which is helpful for estimating the position and size of surface source areas and the relative contribution of passive scalar sources to the measured fluxes. In this study, we analyzed the variations of CO2 flux on different time scales based on the observed data from eddy covariance system. The Kljun footprint model was used to analyze the flux source of the observation data of a rice-wheat rotation cropland ecosystem in Shouxian County, Anhui Province from November 1, 2020 to October 30, 2021. The flux source of different atmospheric conditions and different growth stages of crops were examined. We found that the CO2 flux had obvious temporal variations. The annual variation of CO2 flux featured a W-typed bimodal absorption peak, and the annual average CO2 flux was -0.81 μmol·m-2·s-1. The daily mean CO2 flux (-3.7 μmol·m-2·s-1) in vegetative growth and reproductive stages of rice was the smallest, showing the strongest carbon sink capacity, while the daily mean CO2 flux (1.03 μmol·m-2·s-1) of the wheat vegetative growth period was the largest, serving as a carbon source. The southwest wind prevailed in this region, followed by southeast wind. The maximum length of the flux source area was thus mainly distributed in the southwest and southeast. When the contribution rate of flux was 80%, the maximum length of annual flux source was 158.17 m. The range of flux source area under atmospheric stable state was larger than that under atmospheric unstable state. The flux source area at different growth stages of crops was significantly different. The flux source area of winter wheat vegetative growth stage was the largest, while the source area of winter wheat vegetative growth and reproductive stage was the smallest. The accurate simulation of flux source area of the rice-wheat rotation agroecosystem in Shouxian County plays a vital role for the upscaling exercises of flux data from single site flux measurements to regional scale. Understanding the operation of the flux source area model on cropland underlying surfaces in Huaihe River Basin is also of great practical significance to improve the accuracy of greenhouse gas budget in this area.

    Carbon source and sink characteristics and climate impacts of summer maize cropland ecosystem.
    PENG Jiyong, ZHANG Xihe, WANG Bing, YU Weidong
    2024, 43(10):  3121-3131.  doi:10.13292/j.1000-4890.202410.013
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    Cropland is an important type of terrestrial ecosystem. Maize is the grain crop with the largest planting area and production in China. In the context of climate change, accurately evaluating the carbon source and sink characteristics of summer maize cropland is of great significance to improving cropland carbon sequestration capacity. In this study, we collected the meteorological data during 1991-2022 from Zhengzhou National Basic Meteorological Station and the data of CO2 flux, meteorological factors, and biomass and yield of summer maize during 2008-2022 from Zhengzhou Agrometeorology Experiment Station. Based on these data, we analyzed the change characteristics of climate variables and CO2 flux and the carbon source and sink characteristics of summer maize cropland. The results showed that there were obvious daily and seasonal change characteristics of CO2 flux of summer maize cropland ecosystem. It was a carbon sink from July to September but a carbon source in June. The CO2 flux of summer maize cropland was different under different climate year types. The annual average of gross primary productivity (GPP) and ecosystem respiration (Reco) of summer maize cropland was 1197.5±129.9 g C·m-2·a-1 and 711.2±129.9 g C·m-2·a-1, respectively. Without including grains after harvest, summer maize cropland ecosystem was a carbon sink, with multi-year mean value of net ecosystem exchange (NEE) being -342.8±52.3 g C·m-2·a-1. After the grains were harvested and removed from the cropland, summer maize cropland exhibited a weak carbon sink, with a net biome productivity (NBP) of -41.3 g C·m-2·a-1 (12.0% of NEE) based on calculated theoretical yield. Based on actual yield, summer maize cropland exhibited a strong carbon sink, with a NBP of -105.9 g C·m-2·a-1 (30.9% of NEE). The climate in this area showed a warm-dry tendency. Temperature and precipitation did not affect CO2 flux of summer maize cropland ecosystem. In contrast, sunshine duration had a significant impact on GPP of summer maize cropland ecosystem (P<0.05), and indirectly affected net ecosystem productivity (NEP) and Reco through GPP (P<0.05). In the summer maize growing season, GPP and NPP increased by 19.4 g C·m-2·a-1 and 6.6 g C·m-2·a-1 respectively when sunshine duration increased by 15.6 h·a-1. Our results indicate that summer maize cropland ecosystem has a large carbon sequestration potential.

    Responses of stoichiometric characteristics of Stipa krylovii and Allium polyrhizum to nitrogen addition, drought, and sowing methods.
    PENG Qingtian, WANG Xiaoguang, HUO Guangwei, Wuyunna, NA Muhan, SHI Yiping, SHEN Yue
    2024, 43(10):  3132-3141.  doi:10.13292/j.1000-4890.202410.029
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    A pot experiment was conducted to investigate the effects of nitrogen addition, drought, and different sowing methods on the adaptive characteristics of Stipa krylovii and Allium polyrhizum in the same habitat. We examined the ecological stoichiometry of the aboveground and belowground parts of both species. The results showed that: (1) Nitrogen addition significantly increased the N content of both species, N∶P in the aboveground part of S. krylovii, N∶P and C∶P in the aboveground part of A. polyrhizum; and decreased the P content in the aboveground part of A. polyrhizum, C∶N in the belowground part of S. krylovii and C∶N in the aboveground part of A. polyrhizum. (2) Drought increased N∶P of both species, C∶P of the aboveground part of S. krylovii and C∶P of the belowground part of A. polyrhizum; and decreased P content of S. krylovii and A. polyrhizum and C∶N of the belowground part of A. polyrhizum. (3) Mixed sowing increased C content of the belowground parts of S. krylovii and A. polyrhizum, N content of the aboveground part of A. polyrhizum, the C∶N and C∶P of S. krylovii and C∶N of the belowground part of A. polyrhizum; and decreased N content of the aboveground part of S. krylovii, N content of the belowground part of A. polyrhizum, P content of S. krylovii, P content of the belowground part of A. polyrhizum, C∶N of the aboveground part and N∶P of the belowground part of A. polyrhizum. In summary, N addition reduced aboveground N use efficiency of S. krylovii and A. polyrhizum, and increased aboveground P use efficiency of A. polyrhizum; drought increased P use efficiency in both species and exacerbated P limitation; mixed sowing increased N and P use efficiency of S. krylovii. Under the conditions with increasing nitrogen deposition and drought, the growth of both species will be increasingly limited by P.

    Spatial and temporal variations of bird diversity and bird-strike prevention tactics at Wuhan Tianhe International Airport.
    ZHANG Jun, ZHANG Jie, GAO Yun, WAN Xia
    2024, 43(10):  3142-3151.  doi:10.13292/j.1000-4890.202410.020
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    Wuhan Tianhe International Airport is one of the largest airports in Central China in terms of shipping business. The surrounding environment of the airport has been significantly improved in recent years, and there is an urgent need to conduct bird diversity survey and bird strike risk assessment to provide a scientific basis for bird strike prevention. From April 2021 to March 2022, birds in six habitats within 8 km of the airfield area and clearance area were surveyed using the sample line method. A total of 18386 birds of 128 species in 44 families and 17 orders were recorded. The bird strike risk assessment was conducted. The results showed that 24 species were identified at extremely high-risk bird-strike level, 13 species at high-risk bird-strike level, 26 species at medium-risk bird-strike level, 65 species at low-risk bird-strike level, and 68.42% of the extremely high-risk bird species were waterbird. Temporally, the values of the Shannon diversity index, Pielou evenness index and Simpson dominance index were spring > summer > winter > autumn. Spatially, the highest diversity index was found in the wetland-woodland habitat (H=3.37), the lowest in the residential-woodland habitat (H=2.40), and the other four habitat types in the order of wetland (H=3.00) > residential-wetland (H=2.97)>woodland (H=2.81) > airport lawn (H=2.76). Redundancy analysis showed that the contribution and explanation of wetland area to the distribution of birds in the clearance area were 43.4% and 68.6%, respectively. Therefore, Wuhan Tianhe International Airport needs to strengthen wetland management in the clearance area, develop prevention and control strategies for waterfowl, and establish an early warning system for migratory birds in order to further improve the effectiveness of its works in preventing bird strike.

    Behavior patterns of Ciconia boyciana in different habitats of reservoir wetland in the lower reaches of Huaihe River during migratory stopover period.
    LI Yongmin, ZHANG Jianchun, WEI Bing, LI Dongwei, WANG Weigen
    2024, 43(10):  3152-3159.  doi:10.13292/j.1000-4890.202410.009
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    Migration stopover sites play a crucial role in providing the energy needed for long-distance journeys of migratory birds, and thus are essential for the smooth completion of the migration process. From January to February 2023, we recorded the daily behavior rhythms and behavior patterns of Ciconia boyciana at different habitats in Fushan Reservoir, Anhui Province, using instantaneous scanning and focal animal sampling methods. The daily maximum population recorded reached 505, representing more than 5% of the world population. During the stopover period, the main behaviors of Ciconia boyciana in Fushan Reservoir were foraging (53.29%±30.32%), resting (32.81%±26.68%), vigilance (10.45%±6.35%), and grooming (3.45%±2.95%). Foraging was the predominant behavior during the migration stopover period, with three peaks in a day: 07:40-08:40, 12:40-13:40, and 16:40-17:40. In the habitats of the open water surface and the mudflat, the time budget of foraging was the most, while in farmland, time allocation of resting was the highest. The average foraging duration was 86.48±112.70 s, with the highest pecking frequency (8.80±14.72 times) observed in mudflat habitat and the highest success rate of foraging (44.44%±52.71%) recorded in farmland. The average size of their food was 40.24 cm. Fishes were the dominant food types (97.30%), with most being dead (94.44%). On average, the processing time for food was 75.07 s. Food would be pecked or swallowed according to their sizes. The new discovery of migration stopover sites and the study of migration stopover behavior patterns in an artificial reservoir in the lower reaches of Huaihe River provide important information to enrich and improve the migration stopover network and behavioral ecology of Ciconia boyciana.

    Phytoplankton identification and the variation of community structure in Xin’anjiang Reservoir.
    XU Peifan, YANG Meilin, PANG Wanting, MA Jing, WANG Fushun, CHEN Xueping
    2024, 43(10):  3160-3169.  doi:10.13292/j.1000-4890.202410.034
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    To investigate the distribution of phytoplankton as well as their relationship with environmental factors, water samples from different layers were collected in the river and center regions of Xin’anjiang Reservoir. Temporal and vertical variations of phytoplankton were detected using microscopy and Illumina sequencing technique based on eDNA. The results showed that the phytoplankton in the reservoir were mainly composed of Bacillariophyta, Chlorophyta, Cryptota, and Cyanobacteria. The dominant genera at Jiekou section site in the river area of the reservoir were Cyanobium (8.3%-37.5%) and Synechococcus (7.6%-30.3%) in winter, while Cyclotella (29.3%) and Cryptomonas (16.2%) were dominant in spring. The dominant genera of the section in front of dam in winter and spring were Cyanobium (16.0%-60.6%) and Synechococcus (7.8%-33.0%). At different depths, the relative abundance of Chlorella at 5 m depth was 15.2%, which was significantly lower than that in other water layers. The relative abundance of phytoplankton Cyanobium at the 5 m depth (50.7%) was significantly higher than that in the other two layers (25.3% and 43.2%, respectively). Chlorophyll a, water temperature, chemical oxygen demand (CODMn) and total nitrogen were the environmental factors affecting the composition and distribution of planktonic communities. Our findings suggested that eDNA-based technique has great potential in analyzing phytoplankton biodiversity in reservoirs, including prokaryotic algae and eukaryotic algae.

    Stability and influencing factors of peatland ecosystem: A case study of Jinchuan peatland.
    LI Ziwei, MA Qiuying, YAO Xiaohan, YANG Tao, JIANG Haibo, HE Chunguang
    2024, 43(10):  3170-3179.  doi:10.13292/j.1000-4890.202410.028
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    Peatland is one type of ecosystems with the strongest carbon sequestration capacity on earth, the stability maintenance of which is of great significance to the enhancement of wetland carbon sequestration capacity. We examined the stability of Jinchuan peatland, a typical peatland in Longwan National Nature Reserve, Jilin Province. The evaluation index of ecosystem stability was screened by frequency analysis and ecosystem structure-function index method. Based on analytic hierarchy process (AHP), a peatland ecosystem stability evaluation index system was established, and then was used to assess the stability of the Jinchuan peatland. The results showed that: (1) 20 indicators reflecting wetland structure, wetland function, and external environmental spatiotemporal changes were screened to construct an evaluation index system for wetland ecosystem stability. (2) The value of stability index of Jinchuan peatland was 0.84, indicating that the wetland is in a stable state. The fluctuation of wetland hydrological process caused by climate change and agricultural production was the constraint factor affecting stability. (3) The health status index of Jinchuan peatland was 63.59, indicating a sub-healthy status. In order to maintain the stability of Jinchuan peatland and keep healthy, it is suggested to implement land reclamation for returning the surrounding drylands to wetlands, or adjust the agricultural planting structure, transforming drylands into paddy fields or planting aquatic economic crops. Meanwhile, linear drainage ditches should be filled up to improve the drainage rhythm of wetlands.

    Effects of warming on biomass and allocation characteristic of forest trees: A review.
    DU Xulong, CAI Shifeng, LUO Suzhen, LIU Yuanhao, PEI Yun, HUANG Jinxue, YANG Zhijie, XIONG Decheng
    2024, 43(10):  3180-3190.  doi:10.13292/j.1000-4890.202410.023
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    Tree biomass and its allocation characteristics is an important indicator for studying the impact of global warming on forest ecosystems. It not only reflects tree growth under the background of global warming, but also reveals the carbon cycle and carbon allocation dynamics of forest ecosystems. The response of tree biomass and allocation characteristics to global warming is one of the hot and challenging topics in global change research. At present, some studies reported that warming would significantly increase tree biomass, change plant phenology, prolong the growing season, and increase the biomass in high latitude and altitude areas. In contrast, other studies found that warming has no significant effect on tree biomass. In addition, some studies reported that warming will increase belowground biomass by reducing the allocation of aboveground biomass. However, how forest tree biomass and allocation respond to climate warming and the underlying mechanisms have not been fully understood. Here, we systematically expound the research progress of changes in forest tree biomass and allocation characteristics under the background of global warming, aiming to provide reference for indepth research on changes in forest tree biomass and on the response and adaptation mechanism of forests to global change. The following aspects should be strengthened in the future: (1) Examining the effects of climate warming on forest tree biomass and its allocation characteristics with various warming treatments; (2) The effects of changes in tree phenological patterns or growth rates on forest tree biomass; (3) The biomass and allocation characteristics of forest trees under the combined effects of warming and other environmental factors; (4) Improving the accuracy of research by combining model simulation, indoor simulation experiment and field insitu warming experiment.

    A review on microplastics pollution in water under the influence of dams.
    YU Yang, ZHANG Haiping, GUAN Xingzhong, YANG Jia, LU Jingyuan, QU Xiaodong, GAO Bo
    2024, 43(10):  3191-3198.  doi:10.13292/j.1000-4890.202410.033
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    Dams have profound effects on the environmental behaviors of pollutants and hydrological processes of rivers. Microplastics are a type of emerging pollutants, which are widely distributed and difficult to be degraded. The retention of microplastics in the water body in front of dams may lead to ecological risks, which has received wide concerns. Here, we summarize the influence of river damming on the transportation of microplastics in the water system, the vertical and horizontal migration of microplastics in the reservoir, and the changes in the abundance and distribution patterns of microplastics during reservoir operations. To better understand the microplastic pollution in waters under the influence of dams, we further analyzed the effects of reservoir impoundment on the retention of microplastics, as well as the risks brought by the microplastics retained in the reservoir. This review would provide a theoretical basis for the prevention and control of possible microplastic pollution in the reservoir.

    Highlights and hotspots in canopy science: A review based on the 8th International Canopy Conference.
    HU Haixia, MO Yuxuan, AI Yanyu, LU Huazheng, CHEN Yajun, NAKAMURA Akihiro, SONG Liang
    2024, 43(10):  3199-3204.  doi:10.13292/j.1000-4890.202410.041
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    The 8th International Canopy Conference was held in Yunnan, China from 15 to 19 October 2023. This conference brought together prominent researchers, practitioners, and students in canopy science worldwide. There were five main topics: structural characteristics of canopy biodiversity, species interactions in forest canopy, dynamics of matter and energy exchange at the canopy-atmosphere interface, biogeography of forest canopies, and the roles of forest canopies in environmental education and outreach services. By identifying and reviewing the emerging hot topics, we outlined the progress and trends in international canopy research. Currently, canopy science prioritizes understanding the responses of forest canopy to global change from various perspectives and scales and based on traditional empirical studies and systematic analysis of ecological processes. Future research in canopy science should focus on: (1) exploring the integrated roles of forest canopies within the atmosphere-canopy-ground-soil continuum at local to landscape scales through multidisciplinary collaborations and innovative technologies; (2) establishing standardized methods and data collection protocols to enhance data representativeness and comparability; (3) investigating ecosystem processes in forest canopies and their responses to global change; and (4) integrating and advancing environmental education within canopy science to promote the conservation of forest canopy biodiversity and the improvement of ecosystem services.

    The distribution characteristics and regression model of aboveground biomass of Pleioblastus amarus in Nanling Mountain, Gangdong.
    LIN Daxue, ZHAO Houben, LI Zhaojia, HUANG Chunhua, XU Weihua
    2024, 43(10):  3205-3210.  doi:10.13292/j.1000-4890.202410.014
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    Pleioblastus amarus is widely distributed in the Nanling Mountain of Guangdong, and has great potential in carbon sink. Constructing aboveground biomass allometry model of P. amarus is important for the calculation of forest carbon stock and the assessment of carbon sink function. In this study, 45 individuals of P. amarus were randomly sampled, and the biomass of each organ was determined to construct allometry models for different organs and total aboveground biomass (AGB). Diameter at breast height (DBH) was involved in the models as the main predictor and tree height (H) as an additional predictor. The accuracies of different models with or without H were compared. The results showed that 76.1%±0.8% of AGB was allocated to culm, 14.5%±0.5% to twig and 9.4%±0.4% was allocated to leaf. Culm biomass was positively related to DBH (P<0.05), while the biomass of twigs and leaves were negatively related to DBH. The univariate model involving DBH and the bivariate model involving DBH and H had very high accuracies for predicting both culm biomass and AGB, with R2 exceeding 0.95, and the accuracies for predicting twig biomass and leaf biomass were also high, with R2 being 0.888 and 0.684, respectively. Adding H as an additional predictor into the model improved the accuracy of the model prediction to a small extent but resulted in the problem of multicollinearity. There were small differences between AGB estimates using a single model and the sum of different organs. The results suggest that P. amarus tends to allocate biomass to twigs and leaves in the early stage to ensure rapid photosynthesis and lately to culms for stabilizing its status in community. Univariate models with DBH as a single variable are recommended in the estimation of P. amarus AGB in order to reduce workload while having a high accuracy in calculation.

    Identification of key areas of ecological restoration of land space in Dali Prefecture based on ecological security pattern.
    2024, 43(10):  3211-3222.  doi:10.13292/j.1000-4890.202410.017
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    Land space ecological restoration is a systematic project on certain spatial scale, while the identification of key areas is a prerequisite for putting the plan into practice. We constructed the ecological security pattern and identified the key areas of ecological restoration in the land space in Dali Bai Autonomous Prefecture of Yunnan Province, based on morphological spatial pattern analysis (MSPA), landscape connectivity evaluation, integrated valuation of ecosystem services and trade-offs (InVEST) model, and circuit theory. The results showed that: (1) A total of 30 source areas were screened for ecological source areas in Dali Prefecture, with 11, 10, and 9 source areas being classified as the first, second, and third levels, respectively, showing more source areas in the central and western parts of the study region, and less and fragmentation in the eastern part. (2) A total of 62 ecological corridors were categorized into 8, 16 and 38 corridors of the first, second and third levels, respectively, based on the priority calculation. (3) A total of 1341 pinch points were identified, which were mainly distributed in the northeast and were fragmented, requiring protection and integration. A total of 111 ecological obstacle points were identified, mainly distributed in the eastern urban construction area, requiring man-made restoration with different strategies. (4) The areas to be restored were classified into core ecological restoration areas and important ecological restoration areas according to the grading, and into Erhai waterbird habitat areas, corridor penetration areas, and farmland protection and restoration areas according to their characteristics. Consequently, an ecological security optimization pattern of “one screen, four sections, three zones, and multiple corridors” was put forward. By integrating the principles of morphology and ecology, and fully considering artificial and natural factors, we constructed the ecological security pattern of Dali Prefecture, identified and analyzed the key restoration area, and supplemented detailed information to the urban ecological security pattern. Our results would provide reference for the ecological restoration of the land space of Dali Prefecture.

    The construction of county-level ecological security pattern based on circuit theory: A case study in Qixia City, Shandong Province.
    XUE Mingyue, WU Mengquan, ZHENG Longxiao, DING Zhizheng, WANG Shengchen, XIE Aimin, LIU Jiayan, DING Min
    2024, 43(10):  3223-3232.  doi:10.13292/j.1000-4890.202410.021
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    The construction of ecological security pattern at the county level is an important part of current territorial spatial planning, which is of great significance to the construction of regional ecological civilization. Based on the framework of “Extraction of ecological source-construction of comprehensive resistance surface-identification of ecological corridor”, we simulated ecological flow and animal migration in Qixia City of Shandong Province by using the circuit theory, and identify ecological “pinch points”, “barrier points” and ecological restoration zones, making the construction of ecological security pattern more visual, precise and hierarchical. The results showed that: (1) There are 11 ecological source areas and 20 ecological corridors in Qixia City, including 7 major ecological corridors and 13 potential ecological corridors. There are fewer ecological sources and more potential ecological corridors, indicating that the ecological quality of Qixia should be improved. (2) We identified 19 ecological “pinch points” and 16 ecological “obstacle points”, suggesting the spatial imbalance of Qixia’s ecological environment. (3) The area of the first, second, and third-level ecological improvement area in Qixia City was 318.65, 437.21, and 529.44 km2, respectively. All the areas had different degrees of ecological problems. Therefore, it is urgent to improve the ecological environment near the “pinch points”, improve the patency of the ecological corridors, repair the “obstacle points” and “fault points” in different categories and according to local conditions, improve the landscape ecological connectivity among various sources, strengthen the ecological coordination and ecological environment integrity among towns, and promote the sustainable development of the region.