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    10 January 2015, Volume 34 Issue 1
    Effects of different application methods of a water absorbent on the growth and yield of potato.
    HUANG Wei1,4, ZHANG Jun-hua1,4, ZHU Gui-peng2, ZHANG Li-feng3,4, LIU Yu-hua3,4
    2015, 34(1):  1-8. 
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    We analyzed the effects of applying a water absorbent on the growth and yield of potato (Solanum tuberosum cv. Xiabodi). Four treatments were considered, including hole pattern, seed coating, seed dressing with water absorbent, and no water absorbent as the control. The results showed that the emergence rate and seedling rate of potato plants were increased by applying the water absorbent. The emergence rate and seedling rate when the water absorbent was added to the hole were 22% and 4% higher than those in the control, respectively. Plant height and plant width increased as the potato plants grew. The plants grown with water absorbent added to the hole was 44% higher than that of the control during the starch accumulation period. The highest chlorophyll content and photosynthetic rate were found for  plants grown with water absorbent added to the hole. The proline content of plant tissues increased as plants grew, and was lower in control plants than in that grown with water absorbent added to the hole; the proline content in plants in the other treatments was lower than that in the control. The soluble sugar content first increased and then decreased as sugars were transported into the tubers. The plant fresh weight and dry weight showed similar increasing trends; they increased rapidly after the tuber initiation stage in all treatments. At the starch accumulation stage, the fresh and dry weights were higher in the plants grown with water absorbent than in the control plants. The highest yields were from plants with water absorbent added to the hole (57% higher yield than that of the control), followed by plants grown from seeds coated with the water absorbent. These results showed that addition of water absorbent resulted in higher yields of potato cultivated in the dry environment.
    Effects of planting density on plant form and micrometeorology in different types of rice with potted seedlings by mechanical-transplanting method.
    GUO Bao-wei1, ZHU Cong-cong2, ZHU Da-wei1, ZHANG Hong-cheng1**, JIANG Feng1, GE Meng-jie1
    2015, 34(1):  9-17. 
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    In order to study the impacts of planting density of potted rice seedlings by mechanical transplanting method on plant form and micrometeorology, a field experiment was conducted using conventional japonica rice (Nanjing 44, Wuyunjing 24), japonica hybrid rice (Yongyou 8, Changyou 5), and indica hybrid rice (Liangyoupeijiu, II You 084), which have been widely grown in the southern China, with high, medium and low density treatments of different hole spacing in potted seedling mechanicaltransplanting (12, 14, and 16 cm), and the conventional blanket seedling mechanicaltransplanting as control. The results showed that the distances of leaf occiput between flag leaf and top 2nd leaf and between top 3rd leaf and top 4th leaf, effective leaf area ratio, highly effective leaf area ratio, angle of flag leaf, lengths of top 2nd and 3rd upper leaves, leaf angle, lengths of panicle and uppermost two internodes increased with reducing the planting density, while canopy temperature and humidity decreased with reducing the planting density. Conventional japonica rice with the low density and japonica hybrid rice with the medium density had higher canopy light intensities, while indica hybrid rice presented an opposite trend. Compared with the control, rice plants with potted seedlings by mechanical transplanting exhibited a uniform plant form, and canopy temperature and humidity decreased. Combined with the variation characteristics of plant form, conventional japonica rice planting with 12 cm hole spacing could make better use of the resources of temperature and light, japonica hybrid rice planting with the hole spacing of 14 cm could effectively improve the light and ventilation conditions, coordinate grain contradiction, and hybrid indica rice planting with 16 cm hole spacing was not only suitable for mechanical cultivation, but also improved the percentage of spikeforming tillers.
    Effect of planting geometries on canopy structure of spring maize under highdensity condition in North China Plain.
    WU Xia, CHEN Yuan-quan**, SUI Peng, GAO Wang-sheng, YAN Peng, TAO Zhi-qiang
    2015, 34(1):  18-24. 
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    A field experiment was conducted to compare the effects of planting geometries on the canopy structure, functional properties and yield of the spring maize variety Zhengdan958 planted under high density condition. Four planting geometries were adopted: one plant per clump opposite in adjacent rows (DD), one plant per clump staggered in adjacent rows (DC), two plants per clump opposite in adjacent rows (SD), and two plants per clump staggered in adjacent rows (SC). The results indicated that in the SD and SC treatments, the vertical distribution of canopy exhibited a spindleshaped morphological tendency with an accumulation of the dry matter in stem and leaf in the layer of 0-90 cm (under ear leaf) and 90-180 cm (ear leaf), the stemleaf angle under the lamina of the ear increased significantly by 2.4°-3.4°. The treatments clumps with two plants (SD and SC) provided more niche space for each plant and enhanced tolerance to highdensity planting. Compared with the conventional DD planting pattern, leaf area index (LAI) and chlorophyll relative content (SPAD) values before the filling stage for clumped and scattered planting geometries were lower, while the opposite phenomenon occurred at the filling stage. Compared to the control, the grain yields in these clumped and scattered planting treatments were increased by 11.2%-12.7% but were not significantly different, largely due to increased lodging resistance and higher ear number and thousandkernel weight. Our results confirmed that the population structure and shoot morphological structure under highdensity condition were effectively regulated by the clumped and scattered planting geometries, which improved photosynthesis capacity of middledown leaves at the filling stage and revealed a great potential of highyielding structure and tolerance to highdensity planting.
    Effects of nitrogen, phosphorus and potassium fertilizer applications on plant community structure in a degraded alpine steppe.
    YANG Lu-cun1,2, LIU He-chun1,3, LI Chang-bin1,3, LI Fan4, XU Wen-hua1,2, ZHOU Guo-ying1,2**
    2015, 34(1):  25-32. 
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    Effects of nitrogen (N), phosphorus (P) and potassium (K) fertilizations on plant community coverage, plant diversity and economic herbage groups in a degraded alpine steppe on the northern shore of Qinghai Lake was examined during 2011-2013 in the present study. The threeyear results showed that the total plant community coverage of fertilized treatments in the second (2012) and third year (2013) was significantly higher than that in the first year (2011). Meanwhile, plant community coverage also increased as the fertilization continued, but different fertilization treatments showed distinct responses in comparison with no fertilization treatment. All fertilization treatments reduced the species richness of community, and the species richness decreased as the fertilization continued. However, there was no consistent pattern in the change of Simpson index, Shannon index and Pielou evenness index after fertilization. Different economic groups responded distinctly to fertilization. The aboveground biomass of grass and forb species increased significantly (P<0.05) in the second and third years after fertilization, while that of sedge and legume species had no obvious change after fertilization.
    Numerical classification and ordination of wild Castanea mollissima communities in Taikuan River Nature Reserve, Shanxi.
    LIAN Kai-min1, WU Ying-jian1, ZHANG Li2, ZHAO Lu-lu3, ZHANG Feng4**
    2015, 34(1):  33-39. 
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    Based on the data from the field investigation of 42 quadrats with 37 dominant species, the structure, species composition and distribution of wild Castanea mollissima communities in Taikuan River Nature Reserve, Shanxi and the relationship of community and environment were investigated by TWINSPAN and DCA. The results showed that C. mollissima communities were divided into 11 associations by TWINSPAN, and the distribution of the communities could comprehensively reflect the influences of disturbance, slope direction and soil organic matter on the communities. The results by DCA ordination indicated that the important factors impacting the community distribution were slope direction, terrain and soil type. The first axis of DCA ordination mainly represented the influence of the slope and slope direction on the communities. The relationship between the community and the environment, mainly reflected by the second axis, was not obvious; however, the diagonal of DCA ordination reflected the variations of the communities and the thickness of forest floor. Moreover, the DCA ordination analysis showed that the 37 dominant species had similar patterns. To some extent, community type was dependant on the distribution pattern of dominant species.
    Relationship between flower size and leaf size, number of Stellera chamaejasme population of degraded alpine grassland along an altitude gradient.
    ZHANG Qian, ZHAO Cheng-zhang**, DONG Xiao-gang, MA Xiao-li, HOU Zhao-jiang, LI Yu
    2015, 34(1):  40-46. 
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    The relationship between flower size and leaf size and number reflects the plant adaptation strategies in external morphology during the longterm interaction of plants with different environments, and the variation of the relationship reflects plant adaptation to heterogeneous environments. In this study, an investigation was carried out to examine the relationship between flower size and leaf size and number of Stellera chamaejasme along four different altitude gradients in an alpine grassland in the northern slope of Qilian Mountains. The results showed that, with increasing elevation, the height, density, and aboveground biomass of the plant communities displayed a pattern of initial increase and then a decline. The aboveground biomass, plant height and leaf biomass of S. chamaejasme declined gradually, while reproductive allocation, flower size and leaf number both increased gradually. Flower size of S. chamaejasme was significantly positively correlated with leaf number (P<0.01), but negatively with leaf biomass, while there was no significant correlation between flower size and leaf biomass (P>0.05). Therefore, habitat had a significant influence on the dependency among size of flower and leaf size and number. The plant size would be decreased by the environmental stress at high altitude, and both flower size and leaf number are increased while the leaf size is declined to ensure the reproductive success of S. chamaejasme.
     
    Spatial patterns of Glehnia littoralis population on sandy seashore and their responses to artificial beach ridge.
    ZHANG Min1,2,3, YANG Hong-xiao1,2**
    2015, 34(1):  47-52. 
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    Glehnia littoralis is an endangered rare plant species, with natural habitats along the sandy seashore of eastern China. At a sandy coast of Jiaonan, Qingdao, we investigated the pattern and structure of wild G. littoralis populations that grew before and behind an artificial beachridge, so as to elucidate the recruiting mechanism of the wild G. littoralis and relevant responses to such a beach ridge. The results showed that G. littoralis individuals seaward before the ridge were clumped at spatial scales less than 2.5 m. Adults positively associated with seedlings at finer spatial scales, and might associate neutrally, but not negatively at larger scales. G. littoralis individuals behind the ridge had their aggregative scales and degrees shrunk or weakened, and spatial associations between adults and seedlings turned to be loose or even negative at most scales. The ridge was believed to have affected structures of G. littoralis populations as well. The populations before the ridge exhibited higher relative density, seedling abundance and fruiting rates, i.e., recruiting normally, but behind the ridge, they behaved otherwise. The evidence justified that such a beach ridge has adverse effects on recruitment of G. littoralis populations, presumably because it can prevent seawater from inundating and dispersing seeds of G. littoralis along the seashore.
    Dynamic responses of growth and gas exchange parameters of Isatis indigotica seedlings to different concentration amide nitrogen.
    TANG Xiao-qing**, LU Ting-ting, ZHANG Yi, LI Yan, YANG Yue, SHI Sheng-lu, WANG Kang-cai
    2015, 34(1):  53-59. 
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    The responses of the growth and gas exchange parameters of Isatis indigotica seedlings to amide nitrogen applied at different levels and frequencies were studied. The results showed that application of amide nitrogen at a suitable concentration increased leaf chlorophyll relative content (SPAD), net photosynthetic rate (Pn), transpiration rate (Tr) and intercellular carbon dioxide concentration (Ci) in I. indigotica leaves, and obviously improved the taproot diameter, the fresh weights of leaves and roots, in which the promoting effect of application two times of 1.0%-1.5% amide nitrogen was more stronger. It was suggested that 1.0%-1.5% pure urea could be used for improving the biomass accumulation of leaves and roots in the prestage of I. indigotica seedling cultivation.
    Spatial structure diversity of platycladus orientalis plantation in Beijing Jiulong Mountain.
    ZHANG Lian-jin1, HU Yan-bo2, ZHAO Zhong-hua2, SUN Chang-zhong1**
    2015, 34(1):  60-69. 
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    Based on survey data of a 0.32 hm2 plot in Beijing Jiulong Mountain, the spatial structure diversity of a Platycladus orientalis plantation was analyzed using traditional approaches and a bivariate distribution of three structural parameters, i.e., uniform angle index (W), dominance (U) and mingling (M). The community structure was relatively simple. The P. orientalis population was in an obviously dominant position, and in a state of low mingling, insignificant size differentiation and uniform distribution, while other accompanying species were in a state of high mingling and relative disadvantage. The diameter and height distribution of the stand presented as unimodal. 59% of individual trees in the P. orientalis forest were surrounded by the same species, and 22% were surrounded by only one other species among their nearest four neighbors, i.e., the forest was in a state of low mingling. More than half (52%) of the trees in the plot were in a random pattern, in which most trees (57%) were surrounded by the same species, while 50% of the trees in high mixture (mingling=0.75, 1.00) were randomly distributed. The trees of different extents of dominance had a similar frequency, but the number of dominant trees was slightly more than that of nondominants, and most of the dominant trees were in a state of low mingling and random pattern. The spatial diversity in the P. orientalis plantation resulted from mingling and diameter differentiation. Such a result was not only closely related to the development stage of community but also connected to its origin, anthropogenic disturbance and species composition.
    Photosynthetic characteristics of leaf and calyx of Pairs polyphylla var. yunnanensis at different attitudes.
    GAO Cheng-jie, LIU Fang-yan, YANG Wen-yun, ZHOU Jing, TANG Guo-yong, LI Kun**
    2015, 34(1):  70-78. 
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    Photosynthetic characteristics of leaf and calyx of Pairs polyphylla var. yunnanensis in Kunming (2100 m a.s.l.) and Zelong (985 m a.s.l.) of Yunnan Province, Southwest China were investigated in July, 2013. The results showed that the diurnal variations of the net photosynthetic rates (Pn) of leaf and calyx at the two sites exhibited a doublepeak curve with a midday depression of photosynthesis at 14:00. The maximum Pn values of leaf and calyx were observed at 10:00 and 12:00, respectively. The relatively high temperature and low relative humidity attributed to the midday depression of photosynthesis of P. polyphylla var. yunnanensis in Kunming and Zelong, respectively. The daily average Pn, maximum Pn, light saturation point and initial quantum yield of either leaf or calyx in Kunming were higher than in Zelong, whereas the lower light compensation point and dark respiratory rates of leaf and calyx were found in Kunming, which implied a higher photosynthetic capacity of Pairs polyphylla var. yunnanensis in Kunming. The photosynthetic characteristics of calyx and leaf were similar in Kunming, which showed a wide adaptability of P. polyphylla var. yunnanensis to light. The maximum Pn and light saturation point of calyx were significantly lower (P<0.05) than those of leaf in Zelong, while the significantly higher initial quantum yield was observed for calyx, which revealed a narrow adaptability to light and a high utilization efficiency to weak light for calyx of P. polyphylla var. Yunnanensis at the low altitude site. Calyx removal resulted in the decrease of fruit dry mass, and the extent of decrease in Zhelong was significantly smaller than that in Kunming.
     
    Effects of sand burial on survival and photosynthesis characteristics of two Chenopodiaceae annuals.
    QU Hao1**, ZHAO Ha-lin1, ZHOU Rui-lian2, LI Jin1
    2015, 34(1):  79-85. 
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    In order to understand the effects of sand burial on survival and photosynthesis of two Chenopodiaceae annuals (Agriophyllum squarrosum and Corispermum marocarpum) growing in the Horqin Sand Land, and to compare their differences in abilities and photosynthetic physiological mechanisms to resist sand burial, the net photosynthetic rate, stomatal conductance, transpiration rate and water use efficiency of the two species were studied after they were buried at different sand depths for 5, 10, 15 days, and their survival rates were recorded at the end of the growing period. The results showed that, compared to C. marocarpum, A. squarrosum had a stronger ability to resist sand burial. Some of A. squarrosum seedlings survived after burial depth exceeded plant height by 10 cm, while no seedlings of C. marocarpum survived after the burial depth reached 100% of its seedling height. With the increase in burial depth, the survival rates of both A. squarrosum and C. marocarpum decreased; however, the survival rate of C. marocarpum decreased to greater extents. Both species closed their stomata or decreased their openness, increased transpiration rate and water use efficiency as measures to adapt sand burial stress. Net photosynthetic rates of the two species decreased sharply with increased burial depth, indicating that sand burial was very harmful to the photosynthetic system of plants. Compared to C. microphylla, A. squarrosum adapted better to sand burial stress by recovering the net photosynthetic rate to a certain extent when the burial time increased.
    Photosynthetic characteristics of typical desert plant Artemisia ordosica in semiarid region.
    LI Yuan1,2, ZHA Tian-shan1,2, JIA Xin1,2**, QIN Shu-gao1,2, WU Ya-juan1,2, WANG Ben1,2
    2015, 34(1):  86-93. 
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    Using a portable photosynthesis system, we measured the leaf gas exchange characteristics of Artemisia ordosica, a dominant shrub species in semiarid areas of Northwest China, from May to October 2012 to quantify the temporal variations and environmental controls of its photosynthetic characteristics. Results showed that the diurnal variations of net photosynthesis rate (Pn), transpiration rate (E) and water use efficiency (WUE) were all asymmetric around 12:00, being higher in the morning than in the afternoon. Daily mean Pn varied over the growing season, being highest in June \[(8.97±1.73) μmol CO2·m-2·s-1\] and lowest in October \[(2.58±1.32) μmol CO2·m-2·s-1\]. The apparent quantum yield (α) and the carboxylation efficiency (φ) of A. ordosica varied in the range 0.022-0.048 mol·mol-1 and 0.125-0.268 mol·m-2·s-1, respectively. The maximum net photosynthetic rate at the optimum light intensity (Pnmax) and saturation intercellular CO2 concentration (Anmax) occurred on 13 September (24.89 μmol CO2·m-2·s-1) and 31 July (77.23 μmol CO2·m-2·s-1), respectively. The Pnmax was significantly correlated with the atmospheric relative humidity, and Anmax was significantly correlated with vapor pressure deficit and soil volumetric water content at 30 cm depth. Our results indicated that the carbon assimilation of A. ordosica was primarily limited by moisture related factors, and the photosynthetic characteristics of A. ordosica reflected its adaptation to semiarid conditions with low water availability and high summer temperature. In addition, our study provides important eco-physiological parameters that may help develop processbased ecosystem carboncycle models.
     
    Spatial heterogeneity analysis of soil seed bank for degraded grassland Stellera chamaejasme populations based on geostatistics.
    DU Jing, ZHAO Cheng-zhang**, SONG Qing-hua, SHI Yuan-chun
    2015, 34(1):  94-99. 
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    Soil seed bank is the material basis for natural regeneration of vegetation; the spatial heterogeneity of soil seed bank is of important significance for understanding the mechanism of population reproduction and regeneration. Based on field survey and geostatistics method, this paper studied the spatial heterogeneity of soil seed bank in four differentlydegraded grasslands and its relationship with the ground vegetation of Stellera chamaejasme population in the northern slope of Qilian Mountains, Northwest China. Our results showed that the semivariogram models of soil seed bank in the four degraded grassland gradients were nonlinear, showing aggregated distribution. With the aggravation of grassland degradation, the density and range of soil seed bank increased, the sill and structure proportion showed the trend of “U” type, and the spatial heterogeneity of 76.88%-93.75% was caused by the spatial autocorrelation. Furthermore, in both the nodegradation and heavydegradation grasslands, the relationship between ground vegetation density and seed bank density of S. chamaejasme population showed a significant positive correlation; in the light and moderatedegradation grasslands, such relationship did not exist. In the process of grassland degradation, the spatial distribution of soil seed bank was mainly affected by structural factors such as ground vegetation, while grazing and other disturbance factors to a certain extent reduced the spatial autocorrelation.
    Effects of vegetation succession on carbon stock and δ13C in Loess Plateau.
    WAN Hao1,2**, LIU Wei-guo1,3, WEI Jie1
    2015, 34(1):  100-105. 
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    In this study, we investigated the bulk density (BD), water content (WC), organic carbon (SOC), and stable carbon isotope composition (δ13C) of soils collected from different successional stages of steppe vegetation in Yunwu Mountain, Ningxia Province. The δ13C values of the dominant plants in the vegetation were also analyzed. Our aims were to compare the differences in these variables between different vegetation types and to understand the related environmental information. Results showed that the SOC in the surface layer (0-10 cm layer) was 71.7 g·kg-1 in shrub land, being significantly higher than that in grassland (54.6 g·kg-1). The SOC for the 0-20 cm layer in shrub land accounted for 58.1% of the SOC above 1 m, whereas in grassland the SOC in the 0-40 cm layer only accounted for 34.1% of the SOC above 1 m. The shortterm vegetation succession had a significant influence on the δ13C values of SOC in the 0-20 cm layer, particularly in the 0-10 cm layer. The vegetation succession from grassland to shrub land led to a negative shift of 1.4‰ in δ13C values, but there was no significant change below soil depth of 30 cm. Therefore, δ13C might be a useful tool for studying the changes of soil carbon stock and carbon cycle during shortterm vegetation succession of C3 plants. The δ13C values became less negative from leaves to roots, with the deviation being 2.2‰ for shrubs and 1.9‰ for grass. Moreover, for plant roots from 0 to 40 cm depth, the δ13C values changed from -28.15‰ to -26.11‰ in shrub land and from -27.08‰ to -27.57‰ in grassland.
    Effects of Chinese prickly ash plantation on soil water-stable aggregate distribution and organic carbon turnover in karst mountain area.
    LIAO Hong-kai1, LONG Jian1**, LI Juan1,2, ZHANG Wen-juan1,2
    2015, 34(1):  106-113. 
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    Taking two Chinese prickly ash orchards (35 and 14-year-old, respectively) in the karst mountain region of southwest Guizhou as test objects, and using the dryland as the control, we studied the changes of waterstable aggregates and organic carbon in surface soil (0-20 cm), and discussed the response of soil organic carbon turnover to ages of Chinese prickly ash plantation. The contents of soil waterstable aggregates presented in an inverted ‘V’shaped distribution with the decreasing soil aggregate size in two plantation ages, and the distribution of soil waterstable aggregates was dominant in 5-2, 2-1, and 1-0.5 mm sizes. Compared with the dryland, organic carbon and readily oxidizable organic carbon in whole soil and different aggregates increased significantly in Chinese prickly ash plantations, being highest in the 14 years old Chinese prickly ash plantation, while an obvious decrease trend was found in the 35 years old plantation. With the increasing age of the plantations, cumulative mineralization of soil organic carbon decreased, but the soil organic carbon turnover was significantly longer in the 14 years old Chinese prickly ash plantation as compared with the dryland and the 35 years old Chinese prickly ash plantation, indicating that soil organic carbon was easier to accumulate in the 14 years old Chinese prickly ash plantation. After planting Chinese prickly ash in the karst region, the soil played a role of carbon sink at early stage, but transformed into carbon source thereafter. Therefore, we suggested that attention should be paid to long-term maintenance and management of Chinese prickly ash plantations to prevent soil quality decline in karst regions.
    Effect of litter nitrogen storage and nitrogen release of litter decomposition on soil nitrogen in Pinus koraiensis mixed forests.
    CHEN Li-xin1, JIANG Yi1, DUAN Wen-biao1**, BU Fan1, SUN Shuang-hong1, LI Fan-fan1, XU Yi-fan3
    2015, 34(1):  114-121. 
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    The influences of nitrogen storage and nitrogen release from decomposition in undecomposed litter layer (L), fermentation layer (F), humus layer (H) and surface soil layer (S) on soil nitrogen in sprucefirKorean pine mixed forest and birchKorean pine mixed forest in Xiaoxing’anling Mountains were studied by the direct harvesting method from May to October of 2012. The results showed that during the period of study, the litter standing crops of two forest types were 19.43-27.25 and 21.25-24.28 t·hm-2, and nitrogen storage was 287.21-418.22 and 274.81-351.21 kg·hm-2, respectively. The nitrogen concentrations of different layers in two forest types were ranked in the decreasing order of L, F, H, and S. In sprucefirKorean pine mixed forest, both litter standing crop and its nitrogen storage in each layer peaked in May and September; monthly nitrogen storage increased from L to F layer; the nitrogen released from litter decomposition was easily enriched in F and H layers, but less leached into the soil. However, in birchKorean pine mixed forest, both litter standing crop and its nitrogen storage in each layer peaked in May and October; nitrogen storage decreased from L to F layer; nitrogen was easily transported among all litter layers, and nitrogen transported from litter layer into the soil was more than that in sprucefirKorean pine mixed forest. In the two forest types, significant positive correlations were found between the litter standing crops (in L, F and H layers) and nitrogen in S layer, between nitrogen in H layer and nitrogen in S layer, and between the litter standing crops (in L and F layers) and nitrogen in H layer.
    Degradation characteristics of lignin and cellulose of foliar litter at different rainy stages in subtropical evergreen broadleaved forest.
    MA Zhi-liang, GAO Shun, YANG Wan-qin**, WU Fu-zhong
    2015, 34(1):  122-129. 
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    In order to understand the decomposition characteristics of foliar litter as affected by rainy pattern in the subtropical evergreen broadleaved forest region, the degradation of lignin and cellulose in different seasons were investigated in foliar litter of Pinus massoniana, Cunninghamia lanceolata, Cryptomeria fortunei, Cinnamomum camphora, Toona ciliate, and Quercus acutissima, six representative tree species in the subtropical evergreen broadleaved forest at the upper reaches of Yangtze River, by litterbag method. After oneyear decomposition, mass loss rates in foliar litter were in order of T. ciliate (72.09%) > C. fortunei (58.61%) > C. lanceolata (51.07%) > Q. acutissima (50.38%) > P. massoniana (31.08%) > C. camphora (27.75%). During the firstyear decomposition, the dynamics of lignin concentration in foliar litter varied with tree species, while cellulose concentration in foliar litter declined continually. Lignin loss rates in foliar litter were in order of T. ciliate (88.36%), Q. acutissima (61.21%), C. fortunei (56.41%), C. lanceolata (55.44%), C. camphora (37.10%), and P. massoniana (24.10%). Meanwhile, cellulose loss rates in foliar litter were ranked in order of T. ciliate (99.22%), C. lanceolata (90.58%), C. fortunei (86.84%), Q. acutissima (84.70%), C. camphora (76.40%), and P. massoniana (71.30%). Seasonal rainfall had a significant (P<0.01) effect on the degradation rates of lignin and cellulose in foliar litter of the tree species studied. The degradation of lignin and cellulose in foliar litter in the rainy season was higher than in the dry season. Furthermore, both lignin and cellulose degradation of foliar litter in the subtropical region occurred mainly in the rainy season. In the dry season of the first decomposing year, lignin in foliar litter showed a similar degradation trend with cellulose. In the seasonal rainy period, however, the degradation rate of cellulose in foliar litter was faster than that of lignin.
    Effect of pH on cadmium speciation in rhizosphere soil solutions of different cadmium accumulating plants.
    LIAN Mei-hua1,2, SUN Li-na2**, HU Xiao-min1, ZENG Xiang-feng3, GUAN Xue2
    2015, 34(1):  130-137. 
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    In order to understand the effects of pH on Cd speciation in soil solution,  anodic stripping voltammetry (ASV) and Visual MINTEQ 3.0 model were developed to characterize the speciation of Cd in rhizosphere soil solutions of Cdhyperaccumulating ecotype (HE, Sedum alfredii Hance) and non-hyperaccumulating ecotype (NHE, Sedum sarmentosum Bunge) under different soil pH conditions (4.0, 5.5, 7.0 and 8.5). The model simulation results demonstrated that the proportions of Cd2+ in soil solutions of both two plants decreased with the increase of pH. Cd2+ fraction in rhizosphere solution varied between 46.1%-3.2% for HE, and 60.9%-9.9% for NHE. Cd-DOM complexes were the dominant Cd speciation when pH≥5.5. Cd2+ fractions in rhizosphere solution were always greater for NHE than for HE, but Cd-DOM complexes were the reverse. In addition, results measured by ASV showed that labile Cd complexes varied from 5.5% (pH 8.5) to 79.6% (pH 4.0) for HE, and between 11.8%-86.7% for NHE. Moreover, the fractions of labile Cd measured by ASV were significantly correlated with those calculated by the model (R2=0.80); the results verified the suitability of both the experimental and model methods.
    CO2 efflux from two biologically-crusted soils in response to drying-rewetting cycles with different frequencies in the Tengger Desert.
    ZHAO Rong, LI Xiao-jun**, ZHAO Yang, YANG Hao-tian, CHEN Dong
    2015, 34(1):  138-144. 
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    Drying-rewetting (D/R) cycle of soil induced by discontinuous rainfall events is an important factor influencing soil respiration in desert ecosystems. In this study, two patterns of precipitation (same in total precipitation but different in frequency) were applied to two typical biologically-crusted soils in the revegetation area in the southeast fringe of the Tengger Desert, and laboratory incubation experiment with a constant-temperature of 25 ℃ was adopted, to investigate the response of soil CO2 efflux to repeated D/R cycles with different frequencies. The results showed that as drying and rewetting proceeded, the peak and average respiration rates, accumulated carbon release and average soil water content showed a tendency of decrease, and the decreasing rate declined with the extension of drought. The cumulative carbon emissions of both two biologicallycrusted soils under 10 days interval D/R cycling were less than under 20 days interval D/R cycling. Under the same precipitation pattern, the cumulative carbon emission of moss overlying soil was significantly higher than that of the algae overlying soil. These findings indicate that land cover types and precipitation frequency are both the important factors influencing the response of soil CO2 efflux to dryingrewetting cycle in desert ecosystems.
    Relationship between rhizosphere soil fertility and ectomycorrhizal colonization of Tilia amurensis.
    CUI Lei, MU Li-qiang**
    2015, 34(1):  145-149. 
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    The relationship between ectomycorrhizal colonization and rhizosphere soil fertility of Tilia amurensis Rupr. in Laoshan Ecological Station of Maoer Mountain was analyzed by means of Pearson correlation analysis and stepwise regression analysis. The results showed that there were highly significant negative correlations between the ectomycorrhizal colonization rate and four variables including alkaline phosphatase activity, alkaline hydrolysis nitrogen, available phosphorus and soil water contents. Ectomycorrhizal colonization rate showed a highly significant positive correlation with urease activity, and a significant positive correlation with available K content. Alkaline phosphatase activity and soil water content were the main soil fertility factors influencing the ectomycorrhizal colonization rate of T. amurensis.
    Bacterial diversity cultured from shiros of Tricholoma matsutake
    JIANG Hua1,2, HE Cheng-gang2, YU Fu-qiang1**, LIU Pei-gui1, ZHAO Wen-qing1
    2015, 34(1):  150-156. 
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    Matsutake (Tricholoma matsutake) is an important wild edible mushroom with important economic and medicinal value. The sporocarps often occur and develop in shiros. In this study, soils from six shiros of T. matsutake were collected in Yunnan Province and soil dilution plating was used to determine the diversity of bacterial species represented in these samples. Sequencing of 16S rDNA generated 178 sequences from cultured bacteria, representing 38 operational taxonomic units (OTUs) of 4 phyla and 18 genera. The majority of sequences belonged to Proteobacteria (58.43%), Firmicutes (26.40%), Bacteroidetes (10.67%), and Actinobacteria (4.49%). Further analysis revealed that bacteria cultured from these soils were species of Bacillus (Firmicutes, 24.72%), Burkholderia (Proteobacteria, 21.34%), Pseudomonas (Proteobacteria, 11.24%), and Chryseobacterium (Bacteroidetes, 10.67%). Therefore, the diversity of cultured bacteria from the shiros of matsutake in Yunnan Province was abundant.
     
    Vertical distribution pattern of soil organic carbon of Lanlingxi watershed in Three Gorges Reservoir area.
    TIAN Yao-wu1,2, HUANG Zhi-lin2, XIAO Wen-fa2, ZENG Li-xiong2**, XIANG Yong3
    2015, 34(1):  157-161. 
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    Soil is one of the largest pools of terrestrial organic carbon. Land-use types affect not only the storage but also the depth distribution pattern of soil organic carbon (SOC), which plays important roles in global climatic change. The SOC depth distribution and its influencing factors in Lanlingxi watershed in Three Gorges Reservoir were analyzed. The results showed that landuse types significantly affected the SOC depth distribution. The SOC densities in 0-20 cm layer averaged at 8.47, 5.90, 4.65, and 2.64
    kg·m-2 for forests, grasslands, shrub lands, and croplands, respectively, and the SOC densities in the 0-100 cm were 8.47, 5.90, 4.65, and 2.64 kg·m-2 respectively. The percentage of SOC in 0-20 cm layer (relative to the 0-100 cm) averaged at 69.8%, 57.6%, 50.8% and 36.2% for forests, grasslands, shrub lands and croplands, respectively. With increasing soil depth, SOC density decreased rapidly, and the decline rate was fastest in forests. The forests had a shallower SOC depth distribution. On the contrary, grasslands and shrub lands decreased slowly with soil depth, and they had a deeper SOC depth distribution. Land-use type and altitude significantly affected SOC densities in top layer (0-20 cm), but had no obvious effect on that in the deeper layers (>40 cm). Soil mechanical composition had a little effect on SOC density of surface layer (0-20 cm), but had a significant effect in deeper layers (>40 cm). The soil carbon storage was underestimated when SOC in 0-100 cm layer was considered. SOC density would  be increased by 6.2%-16.5% in the study watershed with SOC storage in 0-150 cm layer being considered.
     
    The impacts of dam impoundment on the distribution patterns of soil organic matter and total nitrogen in Lashihai plateau wetland.
    YANG Hong-sheng1, TIAN Kun2**, YAO Xi1, CAO Pin-lin1
    2015, 34(1):  162-167. 
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    Dam enlargement can lead to the increasing distribution area of flooded wetlands. In the present study, soil samples along a flooding gradient in Lashihai Plateau wetland were collected and analyzed in order to understand the distribution patterns of soil organic matter and total nitrogen, and the differences in their contents before and after the dam impoundment were compared. The results showed that compared to the unflooded area, the contents of soil organic matter and total nitrogen in the flooded area were higher. Also, as the flood intensity increased, the contents of organic matter and total nitrogen first increased and then decreased. As far as the same flood intensity was concerned, the soils severely affected by human activities (e.g., abandoned cropland soils) had lower levels of organic matter and total nitrogen than the undisturbed soils
    (e.g., meadow soil). The analysis of variance (ANOVA) showed that the contents of organic matter and total nitrogen were significantly changed in the perennial waterlogging area (P<0.05) but not in the seasonal flooding area with the alternation of wet and dry seasons (P>0.05). Correlation analysis showed that flood intensity was positively correlated with soil organic matter and total nitrogen contents (P<0.01). Also, soil organic matter was positively correlated with total N (P<0.01). The results could provide basic data to evaluate the impacts of dam impoundment on wetland ecosystems and also a theoretical basis for protection and management of plateau wetland ecosystems.
    Spatial-temporal pattern and source of soil available phosphorus in Minjiang River estuarine wetland.
    ZHANG Wen-long1,2,3, ZENG Cong-sheng1,2,3**, CHEN Xiao-yan1, LIN Wei1
    2015, 34(1):  168-174. 
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    To reveal the spatialtemporal pattern and source of soil available phosphorus (AP) across a subtropical tidal marsh, soil AP, total phosphorus (TP), organic phosphorus (Org-P) and inorganic phosphorus (IP) speciation along a hydrological gradient were examined in Minjiang River estuary of eastern China in May, August and November, 2013. The mean concentrations of soil AP in May (3.53±1.15 mg·kg-1) and August (3.23±1.15 mg·kg-1) were significantly higher than that in November (1.96±1.07 mg·kg-1) (P<0.05). The distribution patterns of soil AP in the three periods were similar, which showed a fluctuant decrease from high tidal marsh to middle tidal marsh and the higher value was observed in Phragmites australis soil compared with that in Cyperus malaccensis soil (P<0.05). Additionally, we observed that soil AP decreased with soil depth in high tidal marsh, while it slightly changed with soil depth in middle tidal marsh. Org-P, iron bound phosphorus (Fe-P) and occluded phosphorus (O-P) were considered as main sources of soil AP in the different vegetation growth periods.
    Community analysis of soil nematodes associated with four grassland species in the Inner Mongolian steppe.
    XU Bing, REN Hui-qin, ZHAO Nian-xi**, RUAN Wei-bin, GAO Yu-bao
    2015, 34(1):  175-181. 
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    There is growing interest in whether plant species composition can affect the composition of soil microbial communities or soil fauna communities. In the present study, the effects of three dominant grass species (Stipa grandis, Stipa krylovii and Leymus chinensis) and one companion species (Agropyron cristatum) on the composition and function of nematode communities were studied in a 10month potexperiment with soil from the Inner Mongolian steppe. Our results revealed the following patterns: (1) Nematode compositions, including abundance, diversity and trophic group structure, and nematode maturity index were significantly different among different plant species. Nematode maturity index was lowest in soil conditioned by A. cristatum. (2) Nematode communities could be separated by plant species identity by discriminant analysis. (3) Based on the similarity coefficients, the result of cluster analysis indicated that these samples were clustered into three subgroups: samples conditioned by S. grandis and S. krylovii as the first subgroup, then samples conditioned by A. cristatum as the second subgroup, samples conditioned by L. chinensis as the third subgroup. This ordination closely resembles the phylogenetic structure of the grasses that were used in this study.
    Bird diversity and ecological distribution in Honghaizi Wetland Park of Yijinhuoluo, Inner Mongolia.
    LI Shi-wei, YANG Gui-sheng**, WANG Wei, LI Bo, LIANG Chen-xia, ZHANG Yu-wei
    2015, 34(1):  182-188. 
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    Species diversity and composition of bird community at Honghaizi Wetland Park in Yijinhuoluo were investigated from October 2012 to September 2013. In total, 116 bird species belonging to 36 families and 16 orders were recorded. Fortynine are summer visitors and 9 are winter visitors which represented 42.24% and 7.76% of whole species; 34 are passage migrants and 24 are residents which represented 29.31% and 20.69% of total species, respectively. The habitats of birds were grouped into four types (wetland, woodland, grassland and residential area) according to topography and vegetation types in the survey area. The diversity index and evenness index of bird community were highest in the wetland (3.042 and 0.687, respectively) and lowest in the residential area (0.599 and 0.227, respectively). The dominance index of bird community was lowest in the wetland (0.079) and highest in the residential area (0.766). The similarity index was highest between woodland and grassland (0.667) and lowest between wetland and residential area (0.204). This paper analyzed the relationship between the main dominant bird species and their habitat characteristics, explained the main reasons for the increased bird species number and individual quantity compared to previous years, and inferred that disease occurrence and mortality of birds could be caused by the worsening of water quality.
    Effects of alizarin complexone (ALC) on otolith marking, growth, and survival of Tanichthys albonubes.
    2015, 34(1):  189-194. 
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    The effects of alizarin complexone (ALC) on otolith marking, growth and survival of Tanichthys albonubes were studied, aiming to improve the applicability of this marking technique in tracing T. albonubes for a better understanding of its movement and migration pattern. Larvae, juveniles with the body length of 5.8-10.5 mm (J5s), juveniles with the body length of 10.8-13.2 mm (J10s), and adults of T. albonubes were selected in the experiment, the water temperature was 28-30 ℃, and immerse time was 24 h. Larvae and J5s of T. albonubes exposed to 50 mg·L-1 and 80 mg·L-1 of ALC had a 100% survival rate, but those exposed to 100 mg·L-1 had a mortality rate of 80%. Under the condition of 80 mg·L-1 ALC, growth rings in otoliths detected by fluorescence microscope (GRFM) were found in all the larvae and J5s. Under 80 mg·L-1 and 100 mg·L-1 of ALC, all J10s survived, but the mortality rate of J10s exposed to 150 mg·L-1 of ALC was 44%. GRFM was found in all J10s under 100 mg·L-1 of ALC. Under 100 mg·L-1 and 150 mg·L-1 of ALC, all adult fish survived with GRFM, but under 200 mg·L-1 of ALC all adult fish died. Cultured for 90 days after exposure to ALC, J5s had the similar body weight, SGR, DWG, and 100% survival rate as compared to the controls (P>0.05). It was suggested that the suitable concentrations of ALC treating larvae, J5s, J10s, and adults of T. albonubes for GRFM were 80 mg·L-1, 100 mg·L-1 and 150 mg·L-1, respectively.
     
    Changes of land cover pattern in the source region of Yangtze River based on Markov process.
    DU Ji-zeng1, WANG Gen-xu1**, LI Yuan-shou2
    2015, 34(1):  195-203. 
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    Based on the spatial dataset obtained from the TM remote sensing data in 1986, 2000 and 2014, combined with the field investigation data, the maps of the land cover pattern in the source of Yangtze River were made, and the transition probability between all kinds of land cover was determined by using the amplitude of the land cover pattern changes during the three different periods. In addition, Markov model of land cover change in this region was established and validated by the transition probability. The characteristics and tendency of land cover changes were analyzed and forecasted with the Markov model. The results indicated that the characteristics of land cover change were coincided to Markov process, thus, the Markov model could be used to validly simulate the process of land cover change in the source region of Yangtze River. The degradation trend of land cover in the source region of Yangtze River was extremely evident. The areas of wetland and alpine grassland with highmid coverage continued to decline, while the area of low cover alpine grassland and bare land had consistently been increasing. However, with the establishment of the Three Rivers Headwaters Nature Reserve and the increase of precipitation in this region since 2000, the degradation trend of vegetation has been relieved obviously.
     
    Multi-source remote sensing of ecologica land in BeijingTianjinTangshan Metropolitan based on threshold segmentation method.
    PENG Jian1,2**, LU Hui-ling2, MA Jing1, LIU Yan-xu1
    2015, 34(1):  204-211. 
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    Eco-environmental problems have become increasingly prominent during the process of rapid urbanization in China. Quick remote sensing identification of regional ecological land can provide the basic data for solving the problems in urban agglomeration. In view of the merits and defects of different types of remote sensing data on characterization of a particular type of feature, remote sensing retrieval of regional ecological land with integrated multisource information has become a hot direction of current research. Therefore, this paper took BeijingTianjinTangshan Metropolitan as an example, considering the advantages of DMSP/OLS nighttime lights data and SPOT/VGT data in identifying construction land and vegetation coverage. The study first used SPOT/VGT data to quantitatively identify the forest land, grassland and cropland, then based on DMSP/OLS nighttime lights data to distinguish water body from construction land. The results showed that the overall accuracy of remotely sensed land cover patterns in BeijingTianjinTangshan Metropolitan reached 85.64%, and the Kappa coefficient was 0.771. The identification accuracy of forest land was the highest (90.87%), water body took the second place (78.33%), and grassland was the lowest (70.97%). This method was proved to be effective to solve the shortage of monosource remote sensing data to quickly distinguish various types of ecologica land.
     
    Spatiotemporal variation in the microclimate effects of typical urban wetland in Beijing.
    CUI Li-juan**, KANG Xiao-ming, ZHAO Xin-sheng, LI Wei, MA Mu-yuan, ZHANG Man-yin, WEI Yuan-yun
    2015, 34(1):  212-218. 
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    To study the effect of wetlands on urban air quality, air temperature, humidity, and negative oxygen ion concentration were monitored along transects from wetland to surrounding landscapes in eight typical wetlands of Beijing. The observed results showed that air temperature increased (R2≥0.81, P<0.01) while humidity and negative oxygen ion concentration decreased (R2≥0.83, P<0.01) with increasing the distance from wetlands. At locations 5 km away from the wetlands, air temperature was 4.4 SymbolpB@C higher, while humidity and negative oxygen ion concentration were 12.8% and 27.2% lower than the values above the wetlands. Moreover, lakes depressed air temperature and elevated air humidity of local environment more pronounced than rivers, by about 1 SymbolpB@C and 5%, respectively. These results demonstrated that urban wetlands had the potential to improve local air quality. Therefore, it was suggested to construct more wetlands with appropriate types to make best use of the integrated functions of urban wetlands.
    Urban heat island variation of each district in Shenyang based on TM/ETM+ data.
    WANG Hong-bo1, LI Li-guang1**, ZHAO Zi-qi1, CAI Fu1, WU Jin-wen1, XU Shen-lai2, JIANG Peng3
    2015, 34(1):  219-226. 
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    Base on the Landsat TM/ETM+ data of August in 2001 and 2010, the surface urban heat island (SUHI) intensity in each district within the thirdcircle freeway of Shenyang was calculated. The data of land use types were used to obtain the information of SUHI source and sink, and the relationship between SUHI and land use types, sourcesink area and boundary length, normal differential vegetation index (NDVI), normal differential built-up index (NDBI), modified normal differential water index (MNDWI) were analyzed. Results show that, in 2001, the areas of medium or high SUHI intensity were mainly within the third circle freeway of Shenyang and Sujiatun district, and the areas of strong SUHI intensity were in Tiexi and Huanggu districts. In 2010, medium or high SUHI areas mainly extended towards the south and southwest, and light, medium and high SUHI areas increased significantly as compared with that in 2001. There was a significant negative correlation between SUHI and NDVI, and a significant correlation between SUHI and NDBI. The proportion of heat island source area to total urban area was significantly correlated with the medium or high SUHI, and the sourcesink boundary length was significantly correlated with the light and medium SUHI.
     
    Spatiotemporal patterns of typical landscapes in the Yuqiao watershed, Tianjin.
    CHEN Ping1, LI Chong-wei1**, WANG Zhong-liang1,2, MENG Wei-qing1, BAO Shan-shan1, XIE Hui-jun1
    2015, 34(1):  227-236. 
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    Changes in typical landscapes have an important influence on the ecohydrological processes and thus water environment security at the watershed scale. In this study, we adopted land use/cover change trajectories in combination with landscape ecology approaches, to investigate the spatiotemporal variability in typical landscapes of Yuqiao watershed, Tianjin during the past 30 years based on Landsat MSS images from 1984 to 2013. The land use/covers exhibit remarkable changes, with 50% and 33% decreases in woodland and farmland, respectively. The changes in land use/cover occur largely in north hilly and northern plain regions, representing about 63.11% of the whole watershed, of which about 96.65% of changes is caused by human activity. The variation of typical landscapes has a similar pattern to that of the whole watershed, exhibiting an overall upward trend in landscape fragmentation for each landscape type with lowered connectivity. The landscape diversity reveals a trend of gradual increase and the heterogeneity of landscape pattern is enhanced.
    Effect of salts on methane emission from wetlands: A review.
    FENG Xiao-ping1,2, WANG Yi-dong1**, WANG Bo-qi3, WANG Zhong-liang1,2
    2015, 34(1):  237-246. 
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    In the context of global climate change and human activities, wetlands are facing significant salinization or desalinization, especially in saline wetlands in inland and coastal regions. Wetlands are major natural sources of atmospheric methane. Methane emission of wetlands is the result of production, oxidation and transport of methane. Salts are recognized as one of the key factors controlling methane emission from wetlands. Salinity change may greatly influence watersoil chemistry, plant community composition and productivity, organic matter accumulation rate and microbialmediated biogeochemical processes, thus altering the emissions of methane. This paper reviews the progresses of studies on methane production, oxidation, transport and emission in wetlands. This paper also analyzes the mechanisms of how salts affect the processes of substrate supply, microbial (methanogen and methanotrophs) abundance, activities and community composition, plants, enzyme activities, electron acceptors, soil pH and Eh. Accordingly, five directions are proposed to be considered in the future: 1) integrated studies on the effects of salts on biogeochemical processes of methane emission; 2) coupling influences of salinity background, changing magnitude and speed of salinization on methane emission; 3) how different ions and their diverse associations affect the processes of methane emission; 4) evaluating how salinity affects methanogens and methanotrophs by combing traditional methods based on laboratory culture techniques with molecular biological tools and stable isotope probing techniques; and 5) spatiotemporal variations of the responses of methane emission to salts.
    Research methods of soil animal food web: A review.
    DOU Yong-jing1,2,3, CHANG Liang1,3, WU Dong-hui1,3**
    2015, 34(1):  247-255. 
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    For a long time, most of studies have focused on the aboveground terrestrial ecosystems, and underground ecosystems are poorly known until now. Nowadays, a growing number of ecologists are turning to study the underground ecosystems. Soil animals as an integral part of the ecosystem have attracted more attention of scientists. The underground trophic link is the most important connection of biological members in ecosystem. It is a key carrier of the material cycle and the energy flow. Therefore, the study of soil animal food web becomes a hotspot and frontier in the field of modern underground/soil ecology. Due to the particular properties of soil animals (such as small size, complex feeding habit and hidden habitat), it is difficult to study soil animal food webs in the field, thus choosing a suitable research method is quite important. This paper summarized the study methods in recent years and divided them into three categories: Traditional methods (direct observation, observation in culture experiments, and intestinal contents analysis), common methods (digestive enzyme analysis, fatty acid analysis, stable isotope techniques and compoundspecific isotope analysis), and modern molecular methods (DNAbased approach tracking trophic links and monoclonal antibody technology). With consideration of the properties of different soil fauna and advantages and disadvantages of each method, the methods of fatty acids and stable isotopes were applied more frequently; but with the improvement of molecular species identification technology and data accumulation, the DNAbased tracking approach will become mainstream in soil food web research.
     
    Three levels of defense and anti-defense responses between host plants and herbivorous insects. 
    YU Hai-xin1,2, YE Wen-feng1, SUN Min-qin2, XU Ning2, LOU Shao-zhi2, RAN Jun-xiang2, LOU Yong-gen1**
    2015, 34(1):  256-262. 
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    In the long-term process of evolution, both host plants and herbivorous insects form a series of complex defense and anti-defense strategies. In this paper, these strategies are classified into three levels. First, host plants perceive herbivoreassociated molecular patterns (HAMPs) and then lead to HAMPtriggered immunity (HTI). This defense is effective against some populations of the herbivore species that cannot secrete effectors. Second, some populations of the herbivore species secrete special effectors to suppress HTI in plants, thereby resulting in herbivore colonization. Third, the effectors of certain populations of the herbivore species could be recognized by resistance genes of host plants, leading to effective effectortriggered immunity and resistance to the herbivore. The elucidation of the mechanisms underlying molecular interactions between host plants and herbivores will not only deepen the understanding of the coevolution between plants and herbivores, but also provide important technical guidance for the breeding of resistant crop cultivars.
     
    Review of the effects of ultraviolet radiation on aquatic organisms.
    WANG Jin-qi1,2**, ZHENG You-fei3, XUE Yan4, WU Fang-fang1
    2015, 34(1):  263-273. 
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    The biological effects of ultraviolet radiation on terrestrial plants have been studied extensively. Ultraviolet radiation can also produce a series of negative effects on aquatic organisms. This review summarizes the situation of ultraviolet radiation penetration into the water column and the influencing factors, elaborates the biological effects of ultraviolet radiation on bacterioplankton, picoplankton, phytoplankton, zooplankton, macroalgae, spawn and juvenile fish, and fish and amphibian, and introduces the biological mechanism of ultraviolet radiation on aquatic organisms from the direct and indirect effects. More research should be conducted on the interactive effects of ultraviolet radiation and climate change, acid deposition, water pollution and other environmental factors on aquatic organisms. Its research objects should be extended to more aquatic organisms, such as submerged macrophytes and benthic organisms. These studies will contribute to understanding the evolution, degradation and restoration of aquatic ecosystems.
     
    Brown tide: A new ecosystem disruptive algal bloom. 
    CHEN Yang-hang1, LIANG Jun-rong1,2, CHEN Chang-ping1,2, LIU Guang-fa1,2, GAO Ya-hui1,2**
    2015, 34(1):  274-281. 
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    The pelagophytes Aureococcus anophagefferens and Aureoumbra lagunensis have formed ecosystem disruptive algal blooms in shallow estuaries of the United States and South Africa for more than two decades. The largescale brown tide events in the coastal waters of Hebei Province in recent years make China the third country that is affected by brown tide in the world. These algae are able to utilize a wide variety of organic nutrients and achieve high growth rates at low light and nutrient levels. They have significant negative impacts on the shellfish mariculture industry, economy and recreational industry. We review research progress in the last two decades on morphology, physiology and molecular and genomic characteristics of these brown tide algae, especially the genes that encode many of the key proteins that facilitate bloom formation and persistence. Moreover, the research progress in China about brown tide is summarized. The prospects of the research on brown tide are also discussed.
     
    The establishment, improvement and validation of the potential pollen source strength of maize.
    HU Ning1, JIANG Xiao-dong1, HU Ji-chao1, LU Zong-zhi2, YAO Ke-min1, PEI Xin-wu3**
    2015, 34(1):  282-287. 
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    Potential pollen source is an important parameter in the maize pollen dispersal model. In order to determine the strength of maize potential pollen source, the daily pollen emission and hourly flowering rate were measured. The product was calculated to estimate the hourly pollen emission as the model input of the potential pollen source. On basis of the data obtained from Lishui experiment in 2009, the normal distribution function was used to simulate the diurnal pattern of hourly pollen emission and then air temperature and relative humidity were introduced to modify this model. Firstly, the data obtained from Gongzhuling and Lishui experiments in 2010 were used to validate the normal model. The measured hourly pollen emissions of “Zinuo18” and “Jidan35” in Gongzhuling experiment and “Zinuo18” and “Su608” in Lishui experiment were compared with the simulated values. Results showed that the root mean squared errors (RMSE) between the measured and simulated values of the four maize varieties were 2.24×105, 1.52×105, 1.24×105 and 8.92×104 grain·plant-1, and the determination coefficients (R2) were 0.330, 0.545, 0.365 and 0.606. Then the same data were used to validate the model modified by air temperature and relative humidity. Results showed that RMSE between the measured and simulated values of the four maize varieties decreased to 1.25×105, 9.03×104, 5.14×104, and 4.22×104 grain·plant-1, and R2 values increased to 0.656, 0.718, 0.779 and 0.843. Model was obviously improved, which could greatly increase the accuracy of the maize pollen dispersal model.
    Simulation of rainfall interception of Qinghai spruce (Picea crassifolia) forest in the eastern part of Qilian Mountains by Gash model.
    GAO Chan-chan1, PENG Huan-hua2, ZHAO Chuan-yan1**, LIU Xing-ming3
    2015, 34(1):  288-294. 
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    The improved Gash model (1995) was used to simulate the rainfall interception by Qinghai spruce forest in the eastern part of Qilian Mountains. Firstly, precipitation, rainfall interception and canopy coverage were measured from the end of May to September in 2009. Secondly, canopy evaporation was calculated by PenmanMonteith method. Thirdly, interception was simulated by Gash model at three time scales (i.e., the observed period, 15 days, and rainfall events during the observed period). Finally, sensitive analysis of parameters in Gash model was made. The results showed that the estimated interception value by Gash model at the whole observed period scale was 8.70% lower than the observed value. The estimated values were 18.92% and 14.23% lower than the observed values respectively, at 15 days scale and rainfall event scale. The interception amount by canopy evaporation accounted for 74.94% of total interception, and the adsorption interception amount by canopy accounted for 25.06% of total interception. The ratio of average evaporation capacity to rainfall intensity is an important parameter of Gash model. The paper suggested that canopy evaporation during a precipitation event should play a key role in improving the accuracy of Gash model, and independent rainfall events should be defined for better estimation of rainfall interception by Gash model.
     
    Index system design and evaluation of ecological civilization construction in Jiangsu Province.
    LI Ping-xing**, CHEN Wen, GAO Jin-long
    2015, 34(1):  295-302. 
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    The ecological civilization construction has become the focus of researchers, the government and the public recently. Therefore, it is of great necessity to evaluate the level of ecological civilization construction and elucidate the key restraining factors. Taking Jiangsu Province, a developed coastal province in eastern China, as the case area, the level of ecological civilization construction and key restraining factors were analyzed with the method of multiindex comprehensive evaluation. The index system was composed by five aspects, i.e., ecological economy, ecoenvironment, ecoliving, ecological culture and ecological institution. From previous index systems built by different researchers, government planning and reports, 147 factors were listed firstly. Based on the relevance, pertinence, applicability and availability of each factor on the evaluation of ecological civilization construction in Jiangsu Province, 22 factors were chosen as representing indicators in this research. The level of ecological civilization construction and the progress of 22 indicators were analyzed in 2007 and 2012. Results indicated that the level of ecological civilization construction increased rapidly after 2007, and the levels of ecological culture and ecoliving increased substantially. However, the progress of some factors from the aspects of ecological economy, ecoenvironment and ecological institution was slow. Regarding the ecological civilization construction, Jiangsu was at the uppermiddle location among the nation, but had some disadvantages in the aspects of ecological economy, ecological institution and ecoenvironment. It is concluded that a series of targeting measures should be adopted to promote the ecological civilization construction, such as promoting the transformation of development mode, strengthening eco-environment protection and perfecting ecological institutions.