Abstract: Soil microorganisms play an important role in the ecosystem functioning of wetlands. The study of wetland soil microbial diversity is crucial for understanding the structure and function of wetland ecosystems. In this study, we employed 16S rRNA amplicon high-throughput sequencing technology to analyze the diversity and structure of soil bacterial communities in rhizospher of 12 species across three life forms (trees, shrubs, and herbs) in Zhangze Lake National Urban Wetland Park. We aimed to explore the relationships between soil physicochemical properties and bacterial communities in rhizosphere soils of different life-form plants. The results showed that: (1) both Chao1 index and Shannon index of rhizosphere soil bacterial communities of trees were significantly higher than those of shrubs and herbs. Among the three plant life forms, the rhizosphere soil bacterial communities of tree species showed the highest variations. (2) Proteobacteria was the dominant phylum across all the three life forms (tree, shrub, and herb), with average relative abundance of 30.4%, 32.3%, and 30.5%, respectively. Proteobacteria, Acidobacteria, Gemmatimonadetes, and Actinobacteria together accounted for over 70% of total relative abundance. (3) The predominant genera associated with trees, shrubs, and herbaceous plants were Vicinamibacter, Sphingomonas, and MND1, exhibiting average relative abundance of 6.1%, 4.9%, and 4.7%, respectively. (4) The α diversity of soil bacterial community was positively correlated with soil total nitrogen and organic matter contents, and negatively correlated with soil pH and total potassium content. In summary, plant life forms and soil physicochemical properties were the key factors driving the difference of soil bacterial community structure in the wetland park.

Key words: plant, soil bacteria, community characteristics, Zhangze Lake National Urban Wetland Park