Abstract: Soil microorganisms provide nutrients for plants by decomposing soil organic residues, which plays an important role in ecosystems. To clarify the composition and temporal and spatial variations of soil fungi in tropical rain forest of Hainan Island, we investigated soil fungi in dry season and rainy season of Diaoluo Mountain, Wuzhishan Mountain, Yingeling, Bawangling and Jianfengling rainforest in Hainan Province. The temporal and spatial variations of soil fungal community composition and diversity were analyzed based on high-throughput sequencing technique. The results showed that there were significant differences in fungal composition among tropical rain forests from different sites, and great differences in fungal diversity between the two seasons. Total nitrogen and total phosphorus concentrations were important environmental factors affecting the diversity of fungi in dry and rainy seasons, with an explanation rate of 13.07% and 17.86%, respectively. Collectively, soil fungal community in tropical rain forests has obvious temporal and spatial variations, which are mainly driven by soil nutrients.

Key words: tropical rain forest, soil, fungi, diversity, season.