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生态学杂志 ›› 2025, Vol. 44 ›› Issue (9): 3143-3151.doi: 10.13292/j.1000-4890.202509.032

• 技术与方法 • 上一篇    下一篇

一种耐低温固定化产碱杆菌对恩诺沙星的降解

张锶1,2,王英刚1*,薛晨阳2,李晓军2*
  

  1. 1沈阳大学环境学院, 沈阳 110044; 2中国科学院沈阳应用生态研究所, 沈阳 110016)
  • 出版日期:2025-09-10 发布日期:2025-09-10

Degradation of enrofloxacin by a low-temperature resistant immobilized Alcaligenes.

ZHANG Si1,2, WANG Yinggang1*, XUE Chenyang2, LI Xiaojun2*   

  1. (1College of Environment, Shenyang University, Shenyang 110044, China; 2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China).

  • Online:2025-09-10 Published:2025-09-10

摘要: 针对北方寒冷地区低温条件下抗生素难降解问题,为进一步提升低温降解菌对环境的适应能力,增强其回收利用率和对污染物的去除效果,本研究以环境中常见的抗生素恩诺沙星(ENR)为目标污染物,利用已研发工艺制备一种耐低温固定化菌剂,评估了该固定化菌剂对环境条件的适应能力和对ENR的降解能力。结果表明:(1)耐低温材料的添加提高了固定化颗粒的机械强度,可达到113.7 N,是常规海藻酸钠颗粒的256.7倍;(2)固定化菌剂削弱了低温的影响,即使在4和8 ℃的低温条件下依然显著提高ENR的去除,去除率比游离菌分别提高11.6%和11.9%;(3)固定化菌剂对pH有较强的适应能力,可在偏碱和偏酸性环境下取得较好的降解效果。在pH=8.0时,固定化菌对恩诺沙星的去除率较游离菌提高22.9%。固定化菌剂进一步提升了低温菌对北方寒冷地区环境条件的适应能力和功能,可为我国北方地区低温条件下抗生素的高效降解提供参考。


关键词: 固定化, 恩诺沙星, 低温菌, 生物降解

Abstract: Antibiotics are difficult to degrade under low temperature in cold regions of northern China. To improve the adaptability of low-temperature degrading bacteria to the environment with low temperature and enhance recycling rate and removal effect on pollutants, enrofloxacin (ENR), a common antibiotic in the environment, was taken as the target pollutant. A low-temperature resistant immobilized bactericide was prepared using the developed process. We examined the adaptability of this immobilized bactericide to environmental conditions and its degradation ability on ENR. The results showed that: (1) The mechanical strength of the immobilized particles was increased by the addition of low-temperature resistant materials. The highest value of the mechanical strength was 113.7 N, being 256.7 times that of the conventional sodium alginate particles. (2) The immobilized bactericide weakened the influence of low temperature. The removal rate of ENR was significantly improved even at 4 and 8 ℃, with the removal rate being 11.6% and 11.9% higher than that of free-living bacteria. (3) The immobilized bactericide has a stronger adaptability to pH, and can achieve better degradation effect in slightly alkaline and acidic environments. At pH=8.0, the removal rate of ENR by immobilized bacteria was 22.9% higher than that by free-living bacteria. The immobilized bactericides further improved the adaptability and function of cryogenic bacteria to the low temperature. Our results could provide a reference for the efficient degradation of antibiotics under low temperature conditions in the northern region of China.


Key words: immobilization, enrofloxacin, psychrophile, biodegradation