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生态学杂志 ›› 2022, Vol. 41 ›› Issue (10): 1969-1976.doi: 10.13292/j.1000-4890.202210.013

• 研究报告 • 上一篇    下一篇

微塑料对尖吻鲈幼鱼生长和肝脏抗氧化系统的影响

谢木娇1,3,徐鹏1,3,周卫国1,2,丁德文1,2,索安宁1,2*


  

  1. (1中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广州 510301; 2南方海洋科学与工程广东省实验室(广州), 广州 511458; 3中国科学院大学, 北京 100049)

  • 出版日期:2022-10-10 发布日期:2022-10-12

Effects of microplastics on growth and liver antioxidant system of Lates calcarifer.

XIE Mu-jiao1,3, XU Peng1,3, ZHOU Wei-guo1,2, DING De-wen1,2, SUO An-ning1,2*   

  1. (1 CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 2Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; 3University of Chinese Academy of Sciences, Beijing 100049, China).

  • Online:2022-10-10 Published:2022-10-12

摘要: 为评估微塑料对海洋鱼类的影响,选取长3 mm × 宽3 mm的聚乙烯和生物降解微塑料对尖吻鲈(Lates calcarifer)幼鱼(初始体重8~12 g,体长7~10 cm)在环境相关浓度(0.375 n·L-1)下进行为期45天的摄食试验,在第30天和第45天取样测定肝脏抗氧化酶活力以及关键通路基因的表达情况。结果表明:两种微塑料均被幼鱼摄食,但对鱼体体重体长的增长无明显影响;微塑料暴露对抗氧化酶活性影响不大,仅在第45天时PE组过氧化氢酶(CAT)活性较对照组显著下降;两个实验组的Kelch样环氧氯丙烷相关蛋白-1(Keap1)、核因子E2相关因子2(Nrf2)、谷胱甘肽硫转移酶1(GST1)和血红素氧合酶-1(HO-1)基因相对表达量在第30天时较对照组升高,但差异不显著;两种微塑料的生物效应无明显区别。环境相关暴露浓度下微塑料对尖吻鲈幼鱼的生长无明显影响,未引起明显的氧化应激。生物降解塑料可被鱼类摄食并在短期内不会被降解,如果在海洋环境积累,对鱼类构成潜在影响。今后需要评估长期摄食微塑料包括生物降解微塑料对海洋鱼类的效应。


关键词: 尖吻鲈, 生物降解微塑料, Keap1/Nrf2

Abstract: To evaluate the effects of microplastics on marine fish, we exposed juvenile Lates calcarifer (initial weight 8-12 g, body length 7-10 cm) in polyethylene  and biodegradable  microplastics (3 mm long × 3 mm wide) at an environmental relevant concentration (0.375 n·L-1) to diet for 45 days. Liver antioxidant enzyme activities and the expression of genes involving in key pathway were measured after microplastics exposure for 30 and 45 days, respectively. The results showed that both types of microplastics could be ingested by fish but did not influence body weight and body length. Antioxidant enzyme activity showed limited responses to microplastics, and only catalase (CAT) decreased significantly in PE group compared after 45 days exposure. After 30 days exposure, all the relative expressions of kelch-like epichlorohydrinassociated protein 1 (Keap1), nuclear factor-E2-related factor 2 (Nrf2), gluthione S-transferase1 (GST1) and heme oxygenase 1 (HO1) increased in both exposure groups but the differences were not statistically significant. There were no significant differences in biological effects between two types of microplastics. Those results indicated that the exposure to microplastics at environmental relevant concentration did not affect the growth and did not cause obvious antioxidant response of juvenile L. calcarifer. Biodegradable microplastics can be ingested by fish and will not be degraded in the short term. If biodegradable microplastics accumulate in the marine environment, they may negatively affect fish in the long term. The current results underlined the need to assess the long-term impacts of biodegradable and conventional microplastics on marine fish.


Key words: Lates calcarifer, biodegradable microplastics, Keap1/Nrf2.