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Table of Content

    08 March 2011, Volume 30 Issue 03
    Articles
    Effects of Cu on Paeonia ostii growth, physiology, and element absorption and allocation.
    2011, 30(03):  415-423. 
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    A pot experiment was conducted to study the tolerance of Paeonia ostii to different levels (low, 300 mg·kg-1; medium, 600 mg·kg-1; and high, 900 mg·kg-1) of added Cu. P. ostii had strong tolerance to Cu. The plant height and root collar growth, taproot thickening and elongation, and biomass of roots and shoots were less affected. Under the Cu treatments, the tolerance index of roots and shoots was higher than 0.5. The superoxide dismutase (SOD) activity in roots decreased significantly under the Cu treatments, but that in leaves increased significantly under low Cu treatment. With increasing concentration of added Cu, the peroxidase (POD) activity in roots and leaves decreased after an initial increase. The MDA both in roots and in leaves did not have a significant increase under low and medium Cu treatments. The soluble sugar and soluble protein contents in roots and leaves increased with increasing concentration of added Cu, while the contents of chlorophyll a, b, and a+b and the chlorophyll a/b ratio were peaked at medium Cu treatment. With increasing concentration of added Cu, the Cu content in roots and stems increased, while that in leaves decreased after an initial increase. The Cu enrichment coefficient in each part of the plant under the Cu treatments was less than 1. The Cu treatments led to a significant reduction in the P and Ca absorption, but promoted the upward translocation of absorbed P and K. Low and medium Cu treatments promoted the Mg accumulation in leaves, but high Cu treatment inhibited it. The Fe and Mn accumulation in roots was inhibited under the Cu treatments, while the Fe and B accumulation in leaves and the upward translocation of absorbed Fe, Mn, Zn, and B were promoted under low and medium Cu treatments. The accumulation of Zn in each part of P. ostii was less affected by the Cu treatments.
    Degradation dynamics of 2,4-dichlorophenol in soil and river sediment.
    2011, 30(03):  424-429. 
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    Soil and sediment samples were collected from the sites along the banks of the fourth Liuhe River and in the River in Nanjing Chemical Industrial Park, and, through soil sterilization and the controlling of temperature and 2,4-DCP initial concentration, the degradation dynamics of 2,4-dichlorophenol (2,4-DCP) in the soils and river sediment and related affecting factors were studied. Microbes played dominant roles in degrading 2,4-DCP. Within 45 d, the degradation rate of 2,4-DCP in non-sterilized soils and river sediment was 1.5-3 and 1.4-2.8 times of that in sterilized soils and river sediment, respectively. The microbe-degraded amount of 2,4-DCP in the soils and river sediment was 0.128-0.599 and 0.113-0.718 mg·kg-1, respectively, and the half-life time of 2,4-DCP was shorter in non-sterilized than in sterilized treatment. Within the range of (10±1) ℃-(30±1) ℃, the degradation of 2,4-DCP was accelerated with increasing temperature. At (30±1) ℃, the residual amount of 2,4-DCP in the soils and river sediment was 0.305 and 0.203 mg·kg-1, respectively, and the residual amount and half-life time were the smallest and shortest. The degradation speed of 2,4-DCP in the soils and river sediment was the fastest when its initial concentration was 0.5 mg·kg-1. With the increase of 2,4-DCP initial concentration, the degradation speed of 2,4-DCP had a decreasing trend, and the half-life time became longer.
    Community structure of denitrifying bacteria in a hybrid AS-biofilm process under aerobic condition.
    2011, 30(03):  430-437. 
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    By using nirK and nirS as the markers, and with the methods of PCR and denaturing gradient gel electrophoresis (DGGE), this paper studied the community structure of denitrifying bacteria in a hybrid AS-biofilm reactor under aerobic condition. In the samples collected, most of nirK-containing denitrifiers were belonged to the known Rhizobiales bacteria of α-proteobacteria, while the nirS-containing denitrifiers were similar to β-proteobacterium (Rhodocyclales and Burkholderiales) and γ-proteobacterium (Pseudomonadales). Some specific sequences were found in nirS- and nirK-denitrifying communities. The data also demonstrated that the growth patterns of bacteria in biofilm or activated sludge could make a significant shift on the denitrifying community structure. These results would provide fundamental information for the understanding of the denitrificaiton in the hybrid AS-biofilm process under aerobic condition.
    Ecological risk assessment and source analysis of heavy metals in river waters, groundwater along river banks, and river sediments in Shenyang.
    2011, 30(03):  438-447. 
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    An investigation was made on the heavy metals in the river waters, groundwater along river banks, and surface sediments in the rivers in Shenyang. In the meantime, the pollution index and potential ecological risk index were used to evaluate the pollution levels of the heavy metals. The As concentration in the river waters ranged in 0.49-11.9 μg·L-1, and was in the order of Shenyang-Fushun Irrigation Channel > Puhe River > Xihe River > Hunhe River; the Cd concentration ranged in 0.01-0.66 μg·L-1, being in the order of Xihe River > the Shenyang-Fushun Irrigation Channel > Hunhe River > Puhe River; and the Zn concentration ranged in 1.0-115 μg·L-1, with the highest concentration in Shenyang-Fushun Irrigation Channel. The highest concentrations of Pb, Cu, and Cr were found in Xihe River. The single pollution index and Nemero index of the 6 heavy metals in Hunhe River and Puhe River were all less than 1.0, but the two indices of Cr and Cd in Shenyang-Fushun Irrigation Channel were higher than 1.0, classified as at slightly polluted level. The Nemero index of Xihe River in summer was higher than 1.0, classified as at slightly polluted level. The Cd and Cr pollution in the shallow groundwater adjacent to Xihe River was more serious, with the Nemero index higher than 1.0 and even over 2.0, classified as at medium pollution level. All the pollution indices of the deep groundwater and rice field water adjacent to Xihe River were less than 10. The sediments in Puhe River and Hunhe River ranked at the low potential ecological risk for the 6 heavy metals. The Cd in the sediments in Xihe River ranked as very high level risk, and Cu ranked as medium-level risk. The pollution sources could be classified as three categories, i.e., (1) waste water discharge and solid wastes, (2) utilization of phosphorous fertilizers and manure, and (3) pollutants discharged from metallurgical, electro-plating, and stainless steel industries.
    Changes of soil enzyme activities and fungal diversity in rhizosphere soil of Solanum rostratum at different development stages.
    2011, 30(03):  448-452. 
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    In order to understand the effects of Solanum rostratum invasion on soil micro-ecosystem, and to approach the optimum time of controlling this invasion, this paper studied the soil enzyme activities and fungal diversity in the rhizosphere soil of S. rostratum at its different development stages. Plate method was applied to culture the fungi, and titration method and colorimetric method were adopted to determine the activities of soil catalase, peroxidase, polyphenol oxidase, urease, desaturase, invertase, and protease. A total of 22 genera fungi were identified. From the early growth stage to flowering stage of S. rostratum, the fungal diversity increased significantly, and the enzyme activities varied obviously. Pearson analysis showed that there existed significant relationships between soil fungal diversity and the six soil enzyme activities except for peroxidase from the early growth stage to flowering stage, and principal component analysis showed that the fungal diversity and enzyme activities at the flowering, budding, and four-leaf stages had significant differences with those at the early growth stage of S. rostratum and in the control soil. Therefore, with the growth of S. rostratum, the fungal diversity and enzyme activities in rhizosphere soil changed.
    Ecological state of invasive alien plant Solanum rostratum in Beijing.
    2011, 30(03):  453-458. 
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    Field investigation was made on ninety 2 m × 2 m sampling quadrats in Ligezhuang and Xiaocaocun villages of Miyun County, Beijing to analyze the population abundance, frequency, and importance value of invasive alien Solanum rostratum, as well as the species composition and flora characteristics of plant communities, aimed to assess the ecological state and ecological endangering risk of the invasive species based on its growth pattern, ecological advantage, and competitive aspects. In abundance, the S. rostratum was at the third position in Ligezhuang village (a total of 29 plant species), and at the fifth position in Xiaocaocun village (a total of 51 plant species); in frequency, the S. rostratum was at the first position both in Ligezhuang and in Xiaocaocun. The importance value indicated that the S. rostratum both in Ligezhuang and in Xiaocaocun had the greatest comprehensive adaptability. Accordingly, the invasion of S. rostratum in Beijing was still at a stage at which the damage was limited and locally, though the plant had overcome its growth bottleneck. Several species belonged to Poaceae, Compositae, and Moraceae had the potential acting as a counterweight to the progressive expansion of S. rostratum. For ecological intervention strategy,it could be established in the native species with some advantages and somehow predominated in adaptation to ecologically substitute or eradicate the invasion of S. rostratum before it became a single dominant species.
    Effects of nutrient level on phenotypic plasticity of Spartina alterniflora.
    2011, 30(03):  459-463. 
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    A greenhouse experiment was conducted to study the effects of different nutrient levels (high, medium, and low) on the phenotypic plasticity of invasive species Spartina alterniflora. With increasing nutrient level, the branching intensity of S. alterniflora increased, and there was a significant difference between low and other two nutrient levels. The spacer length also increased with increasing nutrient level, but the branching angle was not affected by nutrient level. Nutrient level had significant effects on the biomass allocation pattern. With decreasing nutrient supply, the biomass allocation to aboveground parts (stems and leaves) decreased, whereas that to belowground parts (roots and rhizomes) increased. These results suggested that nutrient level had significant effects on the clonal growth of S. alterniflora, and S. alterniflora showed a strong phenotypic plasticity in response to different nutrient levels.
    Differential response of Iris pseudacorus and Canna incida to water depth gradient.
    2011, 30(03):  464-470. 
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    Field investigation was conducted to study the adaptability of Iris pseudacorus and Canna incida to water depth gradient (10, 30, 50, and 70 cm). It was observed that with the increasing water depth, the tiller numbers of I. pesudacorus and C. incida decreased significantly (P<0.05), but the plant height, leaf length, and leaf width were less affected. Both the chlorophyll content and the root vigor of I. pseudacorus and C. incida decreased with increasing water depth, while the leaf MDA concentration was in adverse. The chlorophyll content, root vigor, and leaf MDA concentration of C. incida differed significantly among different water depths (P<0.05), but only the root vigor of I. pseudacorus had significant difference (P<0.05). Among the chlorophyll fluorescence parameters, the maximal light converting efficiency (Fv/Fm), apparent electron transfer rate (ETR), and photochemical quenching (qP) all decreased significantly with increasing water depth (P<0.05), while the non-photochemical quenching (qN) had a significant increase (P<0.05). The relative electron transfer rate (rETR), potential maximal rETR (rETRm), light use efficiency (α), and tolerance to intense light (Ik) all decreased obviously with increasing water depth. At water depth 10 cm, the values of all test indices were higher for C. incida than for I.pseudacorus; but at deeper water depths, an opposite trend was observed. It was found that deeper water depth had inhibitive effect on I. pseudacorus and C. incida, and the effect was greater on C. incida. Therefore, it should be kept in shallow water depth when using these two plants for wetland plant remediation in favor of the two plants’ growth. The appropriate water depth for I. pseudacorus and C. incida would be 30-70 cm and 10 cm, respectively.
    Impact of wind on seedling’s photosynthesis and transpiration of several common crops in Horqin Sandy Land.
    2011, 30(03):  471-476. 
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    A portable wind tunnel and a Li-6400 system were used to study the effects of different wind velocity (4 and 8 m·s-1) and blowing duration (0, 30, 60
    , 90, and 120 min) on the net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Ts) of Zea mays, Triticum aestivum
    , Glycine m
    ax, and Vigna radiata in Horqin Sandy Land where strong wind in spring often hurts plants. With increasing wind velocity and blowing duration, the Pn and Gs of test crops all had a significant decrease. The Pn and Gs at wind velocity 4 m·s-1 decreased by 3.3%-74.4% and 10.9%
    -51.3%, and at wind velocity 8 m·s-1 decreased by 30.8%-81.0% and 48.3%-88.0%, respectively, compared with the control. Except for Triticum aestivun, the Ts of the other three crops under wind blowing increased, and the increment was larger at wind velocity 4 m·s-1 than at 8 m·s-1. Wind-blowing decreased the water use efficiency of the four crops, and thus, increased the dryness of the crops.
    Winter and spring food habits of Macaca mulatta tcheliensis in Taihangshan National Nature Reserve in Henan Province of China.
    2011, 30(03):  483-488. 
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    Macaca mulatta tcheliensis, a peculiar subspecies of rhesus macaque in China, is occurred the temperate Mt. Taihangshan area. In November 2008-April 2009, 40 piles of the macaque feces and 91 categories of plant specimen were collected from the Taihangshan National Nature Reserve in Jiyuan  of Henan Province, and the micro-histological analysis of feces was used to study the food habits of the rhesus macaque in winter and spring. A total of 21 plant species belonging to 13 families were identified as the food items of the macaque in winter, among these plants, Quercus variabilis, Q. aliena, Eriophorum russeolum, Circaea cordata, and Zelkova schneideriana were the major food items, occupying 55.4% of the eaten plants, while 11 plant species, including Z. sinica, Carpinus cordata, Celastrus orbiculatus, and Diospyros lotus, etc., were the common food items, accounting for 39.1% of the eaten plants. The other 5 plant species were occasionally eaten by the macaque, accounting for 5.2% of the winter diets. In spring, 37 plant species belonging to 24 families were fed by the macaque, among these plants, Q. variabilis, Q. aliena, Poa annua, Z. schneideriana, Z. sinica, Carpinus cordata, C. turczaninowii, Semen hoveniae, Celastrus orbiculatus, and Diospyros lotus were the preferred items, occupying 70.8% of the eaten plants, 14 species including Thalictrum aquilegifolium, Syringa oblata, and S. oblata were the common food items, contributing 23.4% to the eaten plants, and the other 12 species were occasionally consumed by the macaque, accounting for 5.7% of the eaten items. Some of the plant species collected was predated by the macaque both in winter and in spring, but the predated  parts of plants differed between two seasons. In winter, the macaque usually ate burgeon and seed; while in spring, the macaque usually ate leaf and flower. Furthermore, the diet of the macaque was more extensive in spring than that in winter.
    Effects of low temperature at booting stage on the percentage of unfilled grains of major rice varieties in Heilongjiang Province.
    2011, 30(03):  489-493. 
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    Sterile-type cool injury has serious impact on the rice production in Heilongjiang Province. To understand the cold tolerance of major rice varieties in the province and to define the quantitative index of sterile-type cool injury can provide scientific guidance for the local rice safety production. In this paper, four major rice varieties Kendao 12, Longdao 3, Longdao 7, and Kongyu 131 in the province were treated with different low temperatures at booting stage, and the effects of these treatments on the percentages of unfilled grains were analyzed. The results showed that when the average temperature was 15℃, the low temperature duration at booting stage was the key factor for the sterile-type cool injury of rice. Under the same durations of low temperature, the constant temperature 15℃ at day and night affected the test rice varieties most seriously. Under different low temperatures, the threshold value of slight sterile-type cool injury to the test rice varieties was 1-4 days or 5-8 days, and that of severe sterile-type cool injury was 5-8 days or more than 8 days, being varied with different varieties. Among the test four varieties, Kendao 12 and Longdao 3 had the weakest cold tolerance, Longdao 7 was medium, and Kongyu 131 was relatively stronger.
    Effects of storage condition on seed germination of four xeromorphic shrub species from Hexi Corridor.
    2011, 30(03):  477-482. 
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    The seed germination responses of Lycium chinense, L. ruthenicum, He
    dysarum scorpaium, and Nitraria sphaerocarpa from Hexi Corridor were exami
    ned after stored in a cold room (-5 ℃, moist) during winter, in a refrigerator
     
    (4 ℃, moist) for 65 days, and at room temperature (20 ℃, dry) for 6 months. The seeds of L. barbarum, L. ruthenicum, and H. scorpaium after stored moist at -5 ℃ and 4 ℃ had higher germination percentage and rate, and an earlier time to the onset of germination, compared with those stored dry at room temperature. The germination percentage of L. ruthenicum seeds was 80% after stored moist in the cold room and in the refrigerator, and only 5% after stored dry at room temperature. 88% and 65% of H. scorpaium seeds germinated after stored moist in the cold room and in the refrigerator, respectively, and 40% of them germinated after stored dry at room temperature. L. barbarum seeds had a germination percentage of 68% after stored moist in the cold room, but of less than 20% after stored humid in the refrigerator and at room temperature. Storage condition had no significant effects on the seed germination of N. sphaerocarpa, and the maximum germination percentage in all storage treatments ranged from 18% to 27%. Therefore, cold stratification (-5 ℃ and 4 ℃) could be an efficient method to break the seed dormancy for L. barbarum, L. ruthenicum, and H. scorpaium, but not for N. sphaerocarpa.
    Dynamic characteristics of land surface parameters of rainfed maize fields in Northeast China.
    2011, 30(03):  494-501. 
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    By using the continuous flux data, meteorological data, and biological data from Jinzhou agricultural ecosystem research station in 2006, the dynamic characteristics of maize plant height (H), leaf area index (LAI), surface soil water content (SWC), roughness (Z0), and albedo as well as their relationships were investigated, aimed to provide references for setting up dynamic parameterization schemes on maize field surface. The H and LAI were estimated with relative accumulated temperature method, and the Z0 was calculated with wind profile equation under neutral condition. The H increased linearly and slowly before jointing stage, and came quickly to the peak value until milk stage. The change of LAI, which emerged the maximum equal to about 4 at jointing-heading stage and reduced to 1.5 in harvesting time, showed an asymmetrical n-type pattern in the growth period. The SWC was the minimum (12%-20%) in winter and the maximum (30%-36%) in summer, while the surface albedo was greater (0.2-0.3) in cold season (from November to next March), the greatest (0.5-0.7) when ground surface was covered by snow, and the smallest (0.1-0.2) in warm season (from June to September). Albedo was significantly positively correlated to LAI during the growth season. Its monthly mean diurnal pattern showed a U-shape distribution, and was affected by the LAI, canopy color and roughness. At the daytime, albedo was in the order of June < September < August < July. In no-freezing period, the albedo had a significant negative correlation with SWC, but this correlation became weaker with increasing LAI. The Z0 had positive exponential correlations with H and LAI.
    Effects of recent 60 years climate change on net primary productivity of evergreen broad-leaved forest in Tiantong region of Zhejiang Province.
    2011, 30(03):  502-507. 
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    Based on the 1954-2009 daily meteorological data of Tiantong region,and using Zhou Guangsheng’s net primary productivity (NPP) model, this paper studied the recent 60 years climate change pattern and its effects on the NPP of evergreen broad-leaved forest in the region. In recent 60 years, the monthly average values of air temperature, precipitation, and reference evaportranspiration (ET0) in the region had an obvious increase in July and August, and the annual mean air temperature, precipitation, and ET0 increased significantly (P<0.0001). The NPP of the evergreen broad-leaved forest in 56 years was averagely 12.196 t·hm-2·a-1, and increased significantly in recent 60 years (P<0.0001). In the future, if the air temperature increased by 2 ℃ and the precipitation increased by 20%, the NPP of the evergreen broad-leaved forest in the region would be increased by 15.9%; if the air temperature increased by 2 ℃2.1 and the precipitation decreased by 20%, the NPP would be decreased by 4.9%; and, if the air temperature increased by 2 ℃ and the precipitation was unchanged, the NPP would be increased by 5.5%. Annual average precipitation, ET0, and air temperature were the main meteorological factors affecting the NPP of evergreen broad-leaved forest in the region.
    Niches and interspecific associations of dominant tree populations at different restoration stages of monsoonal broad-leaved evergreen forest.
    2011, 30(03):  508-515. 
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    Niche and interspecific association are one of the important contents in community dynamics research. Interspecific interaction affects the restoration and succession of community. In this paper, field investigation was made on the community structure of monsoonal broad-leaved evergreen forest at its different restoration stages (primary forest, PF; 15 years restoration, 15R; and 30 years restoration, 30R) in Pu’er City of Yunnan Province, and the niche breadth, niche overlap, and variance ratio (VR) as well as the chi-square test and association coefficient (AC) based on 2×2 contingency table were used to analyze the change trend of the dominant tree populations at different restoration stages. In the three communities at different restoration stages, there was a significant positive correlation (P<0.001) between the importance value and corresponding niche breadth. 78.22% of species pairs at 15R had a niche overlap 0.4-0.8, suggesting a strong resource utilization competition in dominant species; while the community structure at PF and 30R was rather stable, and the species with higher niche breadth had a higher niche overlap than those with lower niche breadth, reflecting that the dominant species had stronger competitiveness. At 15R and 30R, there was a positive interspecific association; while at PF, there was a negative association. In the three communities, most species pairs had no significant association, showed stronger independency, and the proportions of positive and negative associations decreased with the process of restoration. There was a significant positive correlation between the association coefficient and niches overlap at different restoration stages, i.e., the high the niche overlap, the greater the association coefficient.
    Structural control of coastal backbone protection forest belt and its effects on stand canopy and soil.
    2011, 30(03):  516-520. 
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    Taking the coastal Metasequoia glyptostroboides backbone protection forest belt in Pudong District of Shanghai as test object, this paper assessed the changes of stand canopy and soil property after thinning and after planting other trees under thinned stands. The results showed that both the thinned and the two-storied stands promoted the growth of upperstory tree height and diameter at breast height significantly, and also, accelerated the understory vegetation development. The leaf area index (LAI) was the highest in un-thinned M. glyptostroboides stands, followed by in two-storied stands, and in thinned stands. The gap fraction distribution of the three kinds of M. glyptostroboides stand was contrast to LAI. The total N, total P, and available K contents of the soils in the three kinds of M. glyptostroboides stand had no significant differences, while the soil available P, hydrolyzable N, and organic matter contents were higher in thinned and two-storied stands than in un-thinned stands. There was no significant difference in the soil nutrient contents between the thinned and two-storied stands. Forest structure control increased the soil organic carbon (SOC) storage in 0-50 cm layer, and the SOC density was the highest in 0-15 cm layer. The soil respiration in two-storied and thinned stands increased by 42% and 33%, respectively, compared with that in un-thinned stands.
    Decomposition rate of four dominant tree species leaf litters in Qingling Huoditang forests.
    2011, 30(03):  521-526. 
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    By using field litter bag technique, this paper studied the decomposition rate and nutrient release of the leaf litters from Pinus tabulaeformis, P. armandii, Larix principis-rupprechtii, and Quercus aliena var. acuteserrata in Qingling Huoditang forest area. After two years decomposition, the remaining percentage of the leaf litters was 35.6%-58.6%, and ranked as P. tabulaeformis > P. armandii > L. principis-rupprechtii > Q. aliena var. acuteserrata. Except that P. tabulaeformis and P. armandii had no significant difference in their leaf litters remaining percentage, there existed significant differences in the remaining percentage among the test tree species. During the two-year experiment, the leaf litters decomposed fastest during the period from April to September, and kept a stable decomposition rate in other months. Compared the second year, the mass loss rate of the leaf litters in the first year was higher, and showed obvious seasonal and stage-wise difference. The estimation of the required time to decompose 50% and 95% of the leaf litters by the Olson model showed that there was a prominent discrepancy among the tree species. The required time to decompose 95% of leaf litter was the shortest (5.43 years) for Q. aliena var. acuteserrata, and the longest (9.87 years) for P. tabulaeformis. The elements N and P in leaf litters appeared enrichment in  first year of decomposition, and started to release after the  enrichment peak reached; while the C presented a decreasing trend. The differences in  decomposition rate and nutrient release rate among the tree species were mainly related to the initial quality and the nature of the leaf litters.
    Toxicity effect of tributyltin (TBT) on Macrobrachium rosenbergii.
    2011, 30(03):  527-532. 
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    Macrobrachium rosenbergii juveniles and adults were exposed to different concentration tributyltin (TBT) to study the acute and chronic effects of TBT
     on the growth of M. rosenbergii and on the histological structure of the shrimp’s gill and hepatopancrese. For the juvenile and adult of M. rosenbergii, the 96 h median lethal concentration (96 h LC50) was 51 and 376 μg·L-1, and the safe concentration (SC) was 5.1 and 37.6 μg·L-1, respectively. Compared with the control group, the body weight and length of juvenile M. rosenbergii after exposed to 1.25, 2.5, and 5 μg·L-1 of TBT for 30 d decreased significantly (P<0.05), while the growth of adult M. rosenbergii after exposed to 2, 8, and 32 μg·L-1 of TBT for 30 d was less affected, but the histological structure of gill and hepatopancrese appeared abnormal. When the TBT concentration reached 8 μg·L-1, the gill epithelial cells of treated M. rosenbergii swelled, nucleus in support cells contracted, and blood cells in blood cavity aggregated. Correspondingly, liver cells swelled and vacuolated. When the TBT concentration reached 32 μg·L-1, liver cells vacuolated more seriously, nucleus disappeared, and parts of the cells disaggregated and appeared necrosis, showed that definite concentration TBT had strong toxicity to the gill and hepatopancrese of M. rosenbergii.
    Population growth performance and productivity of ciliated protozoa community under two different industrialized grouper larval rearing systems.
    2011, 30(03):  533-538. 
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    An in situ experiment was conducted to study the population growth performance and productivity of protozoa community under two different industrialized grouper larval rearing systems. In the bacteria-added group, 13 new species of protozoa were recorded, and the total population density and the total biomass of protozoa community were 87.3×103 ind·L-1 and 502.9 mg·m-3; in the microalgae-added group, there were 9 new species derived, and the total population density and the total biomass of protozoa community were 60.8×103 ind·L-1 and 70.3 mg·m-3, respectively. For these two groups, Phyllomitus sp., Strobilidium gyrans, and Oxytricha sp. were the dominant species. The species with relatively high population growth rate in the bacteria-added group were Strombidinopsis sp. (0.0578),Halteria sp. (0.0493), Cyclidium litomesum (0.0405), Actinophrys sp. (0.0383), and Mesodimium sp. (0.0373), and those in the microalgae-added group were Lacrymaria sp. (0.0153), Strobilidium gyrans (0.0144), Strombidium sp. (0.0117) and Phyllomitus sp. (0.0100). The total protozoa community productivity varied with rearing-system, being 10.232 mg·m-3·h-1 in bacteria-added group and 1.22 mg·m-3·h-1 in microalgae-added group. In bacteria-added group, Euplotes eurystoma, Strobilidium gyrans, Amoeba sp., and Oxytricha sp. had higher productivity than other species; and in microalgae-added group, the species with relatively higher productivity were Actinophrys sp., Strobilidium gyrans, Phyllomitus sp., and Mesodimium sp. Overall, the ponds with bacteria added had more abundant diversity, higher biomass, and population growth rate of protozoa community than the ponds with microalgae added, and hence, the water culturing regime with bacteria added could attain more live preys during the mouth-opening period of grouper fry. In the cultured water, some species had high population growth rate, but their biomass was not large. The density of these species in breeding ponds was controlled by large zooplanktons, indicating that these plankton species probably played an important role in the balance of artificial ecosystem in the cultured water.
    Age structure, growth and life-history pattern of Schizothorax lissolabiatus in Beipan River.
    2011, 30(03):  539-546. 
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    Schizothorax lissolabiatus is distributed in the Nanpan River, the Beipan River, the Yuanjiang River, the Lancang River, and the Nujiang River. A total of 261 specimens (body length between 105 and 326 mm) were collected from the Beipan River during 2008 and 2009. The characteristics of annuli on scale, opercular, dorsal fin spine and cleithrum of the species were described and compared and the ages of inviduals were diagnosed according to the annuli of scales. The results showed that the relationship between body length (L) and scale radius (R) of S. lissolabiatus was estimated as L=20.202R+65.726, and the body length (L)-weight (W) relationship might be calculated by the power relationship: W=4×10-5L2.8208. The Von Bertalanffy equations of the species were concluated as Lt=475.51[1-e-0.138 (t+1.1397], Wt=1424.93[1-e-0.138(t+1.1397)2.8208; the growth inflection age was year 6.37. The Von Bertalanffy growth function, Logistic growth function and Gompertz growth function of S. lissolabiatus all might describe exactly the growth of its length and weight, and the Von Bertalanffy growth function was the most suitable model to fit  its growth of length and weight. The growth of length of individuals of S. lissolabiatus at the age of 1year to 4 years was much faster than those of more than 5 years. According to the 8ecological parameters of the species, it was concluded that S. lissolabiatus tends to be a r-selected species.
    Gene identification and sequence analysis of Wolbachia in several common mites in citrus orchards.
    2011, 30(03):  547-551. 
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    Polymerase chain reaction (PCR) was adopted to examine the wsp, ftsZ, and 16S rDNA of Wolbachia in Amblyseius orientalis, A. eharai, Euseius nicholsi, Neoseiulus barkeri, Panonychus citri, and Tetranychus harti from the citrus orchards in Nanchang, Jiujiang, and Ganzhou of Jiangxi Province. It was found that only T. harti from the citrus orchards in Nanchang was infected by Wolbachia, with the fragments length of Wolbachia wsp(Accession No. GQ162482), ftsZ (GQ162483), and 16S rDNA (GQ162484) being 593, 758, and 896 bp, respectively, while the other five species were uninfected. The Wolbachia in T. harti was very similar to the group Epo in subgroup B, based on the phylogenetic analysis of wsp gene.
    Pollination biology of Camellia changii.
    2011, 30(03):  552-557. 
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    Camellia changii is an endangered plant species endemic to China, and only confined to the E’huangzhang Nature Reserve in Yangchun City of Guangdong Province. In this paper, an experimental study was conducted on the out-crossing index (OCI), pollen-ovule ratio (P/O), and controlled pollination treatment of natural C. changii  population in the reserve, with the causes of its endangering approached. It was found that C. changii was a typical insect-pollinated plant species, with its breeding system out-crossing and self-incompatible. Its ovary had a high abortion rate (64%) under natural conditions. The style height of the C. changii population was apparently differentiated in length, i.e., 20% were long-morph (type L), and 76% were short-morph (type S). Butterflies and bees were the main effective pollinators of C. changii, but their visiting frequency was low. Based on the experimental results, the major causes of the endangering of C. changii could be (1) the high abortion rate of its ovary due to the self-incompatibility and heterostyly, and (2) the low fruiting rate due to the low visiting frequency of pollinators.
    Effects of host plants and temperature on Frankliniella occidentalis growth and development.
    2011, 30(03):  558-563. 
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    This paper studied the effects of four leguminous host plants and five temperature regimes on the growth and development of Frankliniella occidentalis, and analyzed the start temperature for F. occidentalis development and the effective accumulative temperature for this thrips at its different development stages. At the same temperature regimes, there was a significant difference in the development of F. occidentalis feeding on different host plants, and at different temperature regimes, this difference was not the same. The development of immature F. occidentalis was the fastest when feeding on Phaseolus vulgaris, while the slowest when feeding on Vicia faba, with a significant difference between them. The development of the thrips at each development stage decreased with increasing temperature, and there was a linear relationship between the developmental rate and temperature. The start temperature for F. occidentalis development and the effective accumulative temperature for this thrips at its different development stages differed with host plants, being the lowest (6.35 ℃) and the highest (331.36 degree-days) for the immature F. occidentalis on V. faba, respectively.
    Effects of treating tea plants with exogenous methyl salicylate (MeSA) on the main pests and their natural enemies in tea garden.
    2011, 30(03):  564-568. 
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    In this study, different concentrations of methyl salicylate (MeSA) were sprayed on the tea plants in a nuisance-free tea garden, and yellow sticky trap was used to investigate the population densities of pests and their natural enemies. No significant effect was observed after 24 h MeSA treatment. However, after 48 h, there was a significant decline in the number of tea green leafhopper and a significant increase in the number of ladybug, spider, syrphid, and parasitoids, as compared with the control, which indicated that exogenous MeSA could induce the natural defense of tea plants to the pests, and further, affect the population densities of pest species and their natural enemies in tea garden.
    Estimation of indirect values of forest ecosystem services in a typical forest area of Qilian Mountains.
    2011, 30(03):  569-575. 
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    By using conventional and surrogate market approaches, estimation was made on the indirect monetary values of forest ecosystem services, including organic matter production, water and soil conservation, CO2 fixation, and air purification, in Haxi forest area at the east part of northern slope Qilian Mountains. In the meantime, the ecosystem services values of three vegetation types were compared. The total value of the five ecosystem services in the forest area was 5.2061×108 yuan, among which, woodland occupied 1.951034×108 yuan, shrub land occupied 2.520001×108 yuan, and grassland occupied 0.735065×108 yuan. Calculated by forest land area, the monetary value of indirect ecological services provided by the forest ecosystem in Qilian Mountains was as 3-5 times as that of the Gross National Product in Tianzhu County. Among the three vegetation types, shrub land had the largest monetary values of water and soil conservation services.
    Land use change and its driving forces in Zhanggutai district of south Horqin Sandy Land based on remote sensing and GIS.
    2011, 30(03):  576-583. 
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    By using remote sensing images, GIS technology, and mathematical statistic methods, this paper analyzed the past 20 years’ land use change and its driving forces in Zhanggutai district of Liaoning Province, China. In the period of 1988-2009, cultivated land and forestland were the main land use types in the district. Across this period, the areas of forestland, cultivated land, and construction land increased, while those of grassland, water body, and unused land decreased. The annual change rates of grassland, construction land, and unused land were higher than those of other land use types. The comprehensive index of land use degree increased from 235.34 in 1988 to 251.65 in 2009. The conversion between land use types was mainly characterized by the conversion from grassland and unused land to forestland and cultivated land and that between cultivated land and forestland, accounting for 95.58% of the total conversed area. These land use changes were driven by the combined effects of population, policy, and environmental conditions, among which, population growth and policy guidance played critical roles in intensifying these changes at 20-year time scale.
    Conservation of biodiversity under impact of human activities: Vegetation evolution in Central Europe and its implication.
    2011, 30(03):  584-588. 
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    The studies on the vegetation history in Central Europe showed that human activities not only disturbed the nature, inducing the simplification of vegetation, but also gave impact on the abundance (diversity) of vegetation and landscape. The disturbances of human beings and live stocks could make some semi-natural and high diversity habitat types able to be maintained and developed, but excessive disturbances would certainly induce the simplification and sparseness of vegetation, giving threats to the conservation of biodiversity. Today’s vegetation patterns in Central Europe are in general mostly the products of centuries or, in places, even millennia human disturbances in addition to climate change impact. In most areas of Central Europe, the potential vegetation was mostly forest. Without the strong impact of human activities, most areas of today’s Central Europe, except water biotopes, raised bogs (small remnants of which still exist in certain areas), coastal regions, and alpine biotopes above treelines, would still be covered by more or less closed hardwoods, instead of a diversified landscape. Therefore, the objective of nature conservation should not be limited to “purely natural” vegetation, and the method of approach should not be limited to “remove human impact”. All the vegetation types with typical features are worthy to be conserved. Besides natural ecosystems with high naturalness, cultivated vegetations are ought to be conserved, otherwise, they will be disappeared due to specified cultivation and management. In the meantime, we should not only conserve the actual vegetation, but also restore the potential vegetation, in order to sustain and develop the current diversified landscape, vegetation, and habitat. The conclusions from this review provide insights into the debates on nature conservation in Europe, and also, give reference to the biodiversity conservation strategy in China.
    Nutrient metabolism of marine macroalgae and its relationship with coastal eutrophication: A review.
    2011, 30(03):  589-595. 
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    Marine macroalgae are the important components of coastal primary productivity, playing a key role in the coastal carbon cycle. In recent years, there is a growing recognition that marine macroalgae could be a good solution of bioremediation to the problem of coastal eutrophication. Coastal eutrophication may lead to the excessive growth in some species of opportunistic macroalgae. Therefore, it is essential to further understand the relationships between marine macroalgae and changes of nutrient supply. This paper reviewed the related ecophysiological issues on the relationships between macroalgae and coastal eutrophication (increasing nutrient supply in seawater), with the focus on the factors affecting the characteristics of nutrient uptake by marine macroalgae, their intercellular storage of nutrients, ecological requirements of nutrients, and ecological responses to the coastal eutrophication. The prospects for future research werealso discussed.
    Soil washing/flushing of contaminated soil: A review.
    2011, 30(03):  596-602. 
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    Soil washing/flushing is an efficient technology for rapid remediation of soils or sediments highly contaminated by heavy metals and/or organic pollutants. In this paper, the characteristics, technical processes, and cleaning agents for soil washing/flushing remediation were reviewed. The ex-site soil washing has broad application prospects, due to its stable remediation efficiency, easy control, and waste reduction. Environment-friendly cleaning agents, such as natural chelating agents and bio-surfactants, have gradually replaced artificial chelating agents and chemical surfactants, and become the main cleaning agents for soil washing/flushing. Modern supramolecular chemistry will have significance in the washing/flushing remediation of soils contaminated by combined pollutants.
    Behavior ecology of great gerbil Rhombomys opimus: A review.
    2011, 30(03):  603-610. 
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    Rhombomys opimus belongs to the subfamily Gerbellinae of family Muridae, order Rodentia. It has a vast distribution range from Iran and Afghanistan via Kazakhstan to China and Mongolia, being a species with the biggest body size in Gerbellinae and a key rodent in the Middle and Central Asian arid zone. R. opimus digs complex burrow system for establishing settlement where a family group of 2-3 generations lives together. R. opimus feeds Haloxylon ammodendron, Tamarix chinensis, and various others plants, giving strong impact on vegetation development and productivity, and consequently, on the structure and processes of desert ecosystem. This paper summarized the research findings on the habitat, feeding behavior, food storage, alertness behavior, territorial behavior, social behavior, and spatial dispersion of R. opimus as well as its activity rhythm during daytime, and discussed the further research contents about this species to understand its biological characteristics better and to supply theoretical and practical bases for the effective control of R. opimus, maintaining the stable and healthy development of desert ecosystem, and the management of desertification.
    Characteristics of forest damage induced by frozen rain and snow in South China:A review. 
    2011, 30(03):  611-620. 
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    In recent years, extreme weather disaster happens frequently, and much attention has been paid to the disasters caused by frozen rain and snow. Behaving as intense disturbances, extreme frozen rain and snow disasters could exert strong effects on forest ecosystems, especially in the subtropical area of China where frozen rain and snow weather rarely happens. In South China, a long-lasting extreme meteorological disaster occurred from January to February 2008. This meteorological disaster was characterized by frozen rain and snow with extreme low temperature, which caused huge damage to the forests across a large region. Many studies have been made in this country on the general situation of this disaster, damaged conditions of forests, and related affecting factors, but the measurement criteria of damaged trees were rather subjective, rough, or incomplete, and the relevant concepts were confusing, which made it difficult to compare the results among different studies. In order to standardize the classification criteria of damaged trees and to clarify relevant concepts, we proposed the physiological and mechanical classification criteria of damaged trees based on previous studies and the climatic and biological characteristics of extreme meteorological disasters, which could be a reference to relevant studies. Meanwhile, the factors that could affect the damage status of forest trees during this disaster were analyzed, and they were summarized into two categories, i.e., one, affecting the weights of frozen snow and ice, and the other, affecting the resistance of trees. In the last, some comments for the forest restoration and reconstruction after the disaster were proposed, based on the affecting factors of the disaster and the secondary damages easily to be happened after the disaster.
    Urban ecologization level: Evaluation method and its application.
    2011, 30(03):  621-628. 
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    A basic variable index system for urban ecologization evaluation was built from the aspects of five competent supporting sub-systems of urban ecosystem, and the technical method for dynamic sieving of the key evaluation index was established. In addition, the concept of urban ecologization index (UEI) was defined, and the corresponding calculation method was proposed. Furthermore, the status box plot of urban ecologization was designed, and the theoretical structure of quantitative analysis on urban ecologization level was constructed. The quantitative evaluation of current ecologicalization status of Shanghai showed that this city is at the initial stage of urban ecologicalization, and its further development is facing double challenge both from integrity and from constitutes.