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Responses of Juniperus sabina and Pinus sylvestris var. mongolica seedlings to shading in low nutrient conditions.

MENG Peng*   

  1. (Liaoning Province SandFixation and Afforestation Research Institute, Fuxin 123000, Liaoning, China).
  • Online:2017-11-10 Published:2017-11-10

Abstract: To determine the effects of shade on survival rate, relative growth rate (RGR), biomass allocation and photosynthesis characteristics, potted seedlings of shadetolerant Juniperus sabina and shadeintolerant Pinus sylvestris var. mongolica in lownutrient substrate were grown without shade or under a 35%, 65% or 95% reduction of full sunlight over two growing seasons. The results showed that, with decreased light availability, J. sabina growth and survival did not change significantly. Total biomass and RGR increased and then decreased; RGR indicators were positive; and the shoot/root ratio increased significantly. In contrast, the survival rate and growth of P. sylvestris var. mongolica decreased significantly. Total biomass and RGR decreased; the RGR was negative under 95% shade; and the shoot/root ratio decreased significantly. The maximum photochemical efficiency (Fv/Fm) of J. sabina was more sensitive to shade and increased significantly under 35% shade, while the Fv/Fm of P. sylvestris var. mongolica increased significantly until 65% shade. A JIPtest showed that the standardized fluorescence values (Vt) of J. sabina under shade were lower than those under the nonshading treatment (CK) from initial O phase to final P phase. Furthermore, the proportion of electron energy across the QA reaction center to the whole energy captured (ψo), quantum yield of electron transfer (ψEo), size of plastoquinone pool (Sm), plastoquinone pool reduction rate (Sm/Tfm), number of reduced primary quinone acceptors (N), and energy for electron transfer per excited crosssection (ETo/CSo) all increased. The density of reaction centers (RC/CSo) increased, while dissipation per excited crosssection (DIo/CSo) and per reaction center (DIo/RC) decreased, leading to significantly higher performance indexes (PIABS, PICSo, PICSm) under shade than under CK. The Vt of P. sylvestris var. mongolica under shade was higher than under CK from O phase to P phase; however, the above fluorescence parameters were lower or were not significantly different under shade except for the heat dissipation indexes. DIo/RC remained unchanged in P. sylvestris var. mongolica under shade, and the performance indexes (PIABS, PICSo and PICSm) also remained unchanged. The above results indicated that J. sabina was able to maintain high growth and survival under low light intensity by maintaining a positive carbon balance and increasing the proportion of aboveground biomass and electron transfer energy levels during the lightreaction stage. In contrast, P. sylvestris var. mongolica had a low survival rate due to a carbon budget imbalance, and the decreases in proportion of aboveground biomass and electron transfer energy level under shading conditions.